zeiss_lsm_980_oeiras
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zeiss_lsm_980_oeiras [2025/03/01 01:30] bioimaging [Objectives] |
zeiss_lsm_980_oeiras [2025/03/01 01:39] (current) bioimaging [Detector(s)] |
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| | <fc #ff0000>639 nm (red)</fc> | 639 nm | 10 mW | <fc #cd5c5c>Cy5, APC, Alexa/ATTO633-647</fc> | | | <fc #ff0000>639 nm (red)</fc> | 639 nm | 10 mW | <fc #cd5c5c>Cy5, APC, Alexa/ATTO633-647</fc> | | ||
| - | If you require more laser lines (eg, 445nm - CFP/Cerulean, 514nm - YFP, or 594nm mCherry/AF594), we suggest as an alternative the LSM9098-32 in Lisbon: | + | If you require more laser lines (eg, 445nm - CFP/Cerulean, 514nm - YFP, or 594nm mCherry/AF594), we suggest as an alternative the LSM980-32 in Lisbon: |
| https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_980 | https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_980 | ||
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| - | ==== Eyepiece filterset(s) (COLIBRI illumination) ===== | ||
| - | ^ Position ^ Transmission ^ Description (use) ^ | ||
| - | | DAPI | 445/50 nm | <fc #00ffff>DAPI, CFP or Hoechst filter</fc> | | ||
| - | | GFP | 525/50 nm | <fc #00ff00>RFP or TRITC (LongPass)</fc> | | ||
| - | | RFP | LP590 nm | <fc #ffa500>GFP or FITC filter</fc> | | ||
| - | | Mirror | - | Mirror for LSM | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
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| | 2.5x | | - | 0.085 | 8.8 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420320-9902-000/Objective-EC-Plan-Neofluar-2.5x-0.085-M27|420320-9902-000]] | | | 2.5x | | - | 0.085 | 8.8 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420320-9902-000/Objective-EC-Plan-Neofluar-2.5x-0.085-M27|420320-9902-000]] | | ||
| | 10x | EC Plan-Neofluar | Air | 0.45 | 2.0 | [[https://www.micro-shop.zeiss.com/en/ee/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | | 10x | EC Plan-Neofluar | Air | 0.45 | 2.0 | [[https://www.micro-shop.zeiss.com/en/ee/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | ||
| - | | 20x | Plan-Apochromat | Air | 0.75 | 0.55 | [[https://www.micro-shop.zeiss.com/en/ee/shop/objectives/420150-9900-000/Objective-Fluar-20x-0.75-M27]] | | + | | 20x | Plan-Apochromat | Air | 0.75 | 0.55 | [[https://www.micro-shop.zeiss.com/en/ee/shop/objectives/420150-9900-000/Objective-Fluar-20x-0.75-M27|420150-9900-000]] | |
| - | | 25x | | W/Sil/Gly/Oil* | 0.8 | 0.57 | [[https://www.micro-shop.zeiss.com/en/ee/shop/objectives/420852-9870-790/Objective-LD-LCI-Plan-Apochromat-25x-0.8-Imm-autocorr-DIC-M27]] | | + | | 25x | | W/Sil/Gly/Oil* | 0.80 | 0.57 | [[https://www.micro-shop.zeiss.com/en/ee/shop/objectives/420852-9870-790/Objective-LD-LCI-Plan-Apochromat-25x-0.8-Imm-autocorr-DIC-M27|420852-9870-790]] | |
| - | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | + | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | |
| - | | | + | |
| | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
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| 4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE) | 4x GaAsP PMT spectral detectors (tunable between 450-750 nm) (~45% QE) | ||
| - | 1x green-red multialkali PMT (10-45% QE) | + | 1x green-red multialkali PMT (10-25% QE) |
| 1x T-PMT detector (brighfield) | 1x T-PMT detector (brighfield) | ||
| - | 1x Airyscan2 detector (>>45% QE after processing Airyscan data) | + | 1x Airyscan2 detector (~45% QE after processing Airyscan data) |
| ==== Airyscan2 emission filters ===== | ==== Airyscan2 emission filters ===== | ||
| ^ Position ^ Emission ^ | ^ Position ^ Emission ^ | ||
| - | | | | + | | 1 | | |
| - | | | | + | | 2 | | |
| - | | | | + | | 3 | | |
| - | | | | + | | 4 | | |
| - | | | | + | | 5 | | |
| + | | 6 | | | ||
| + | | 7 | | | ||
| + | | 8 | | | ||
| + | |||
| + | ==== Eyepiece filterset(s) (COLIBRI LED illumination) ===== | ||
| + | ^ Position ^ Transmission ^ Description (use) ^ Excitation ^ | ||
| + | | DAPI | 445/50 nm | <fc #00ffff>DAPI, CFP or Hoechst filter</fc> | 385nm | | ||
| + | | GFP | 525/50 nm | <fc #00ff00>RFP or TRITC (LongPass)</fc> | 493nm | | ||
| + | | RFP | LP590 nm | <fc #ffa500>GFP or FITC filter</fc> | 555nm | | ||
| + | | Mirror | - | Mirror for LSM | - | | ||
zeiss_lsm_980_oeiras.1740789008.txt.gz · Last modified: 2025/03/01 01:30 by bioimaging
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