zeiss_lsm_900
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zeiss_lsm_900 [2025/12/09 12:40] bioimaging [How to start your session] |
zeiss_lsm_900 [2025/12/12 14:56] (current) bioimaging [Filtersets (for eyepieces)] |
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| ^ LASER ^ Wavelength ^ Maximum Power ^ | ^ LASER ^ Wavelength ^ Maximum Power ^ | ||
| - | | violet | <fc #a600ff>405 nm</fc> | 15 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 15 mW | |
| - | | blue | <fc #00f7ff>488 nm</fc> | 13 mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 13 mW | |
| - | | yellow-green | <fc #c6ff00>561 nm</fc> | 13 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 13 mW | |
| - | | red | <fc #ff2600>640 nm</fc> | 10 mW | | + | | Red | <fc #ff2600>640 nm</fc> | 10 mW | |
| ==== LEDs (for eyepieces) ==== | ==== LEDs (for eyepieces) ==== | ||
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| | G | <fc #b3ff00>555 nm</fc> | | | G | <fc #b3ff00>555 nm</fc> | | ||
| | R | <fc #ff4f00>630 nm</fc> | | | R | <fc #ff4f00>630 nm</fc> | | ||
| + | |||
| + | ==== Filtersets (for eyepieces) ==== | ||
| + | |||
| + | ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | Green | 450-490 nm | 493 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/000000-1031-346|FS38]] | | ||
| + | | Orange-Red | 533-558 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | | ||
| + | | Blue | 370-410 nm | 420 nm | <fc #0046ff>430-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489096-9100-000|FS96HE]] | | ||
| + | |||
| ==== Objectives ==== | ==== Objectives ==== | ||
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| | <fc #01b3f5>40x</fc> | LD LCI Plan-Apochromat | W, Sil, G | 1.2 | 0.41 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | | <fc #01b3f5>40x</fc> | LD LCI Plan-Apochromat | W, Sil, G | 1.2 | 0.41 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | ||
| | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
| - | |||
| - | |||
| - | ==== Emission Filters ==== | ||
| - | |||
| - | ^ Filter name ^ Transmission ^ Reference ^ | ||
| - | | BFP | <fc #0046ff>450/40 nm</fc> | [[https://www.fpbase.org/spectra/?s=2666,2668,2667,2055&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|96 HE]] | | ||
| - | | GFP | <fc #4aff00>525/50 nm</fc> | [[https://www.fpbase.org/spectra/?s=1484,1495,1485,2057&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|38 HE]] | | ||
| - | | Alexa 546 | <fc #ffdf00>LP590 nm</fc> | [[https://www.fpbase.org/spectra/?s=1530,1533,1536,2059&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|15]] | | ||
| - | | Cy5 | <fc #ff0000>690/50 nm</fc> | [[https://www.fpbase.org/spectra/?s=1547,1550,1543,2068&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|50]] | | ||
| - | | DIC | < 800 nm | - | | ||
| - | | mPlum | <fc #ff5300>629/62 nm</fc> | [[https://www.fpbase.org/spectra/?s=2620,2618,2619,2069&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|64 HE]] | | ||
| - | | Quad | <fc #5400ff>425</fc>/<fc #1aff00>514</fc>/<fc #ffd900>592</fc>/<fc #ef0000>709</fc> nm | [[https://www.fpbase.org/spectra/?s=2657,2601,2602,2658,2577&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|90 HE]] | | ||
| ===== How to start your session ===== | ===== How to start your session ===== | ||
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| * Turn on Systems | * Turn on Systems | ||
| * Turn on Components | * Turn on Components | ||
| - | * Turn on the power bar | ||
| * Turn on the incubation module (if needed - turn on 30 mins before acquiring) | * Turn on the incubation module (if needed - turn on 30 mins before acquiring) | ||
| {{::Oeiras_LSM900_incubation.png?200|}} | {{::Oeiras_LSM900_incubation.png?200|}} | ||
| + | * Turn on the power bar | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| * Start **ZEN** | * Start **ZEN** | ||
zeiss_lsm_900.1765280418.txt.gz · Last modified: 2025/12/09 12:40 by bioimaging
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