zeiss_lsm_900
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zeiss_lsm_900 [2025/12/08 18:16] bioimaging |
zeiss_lsm_900 [2025/12/12 14:56] (current) bioimaging [Filtersets (for eyepieces)] |
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| ^ LASER ^ Wavelength ^ Maximum Power ^ | ^ LASER ^ Wavelength ^ Maximum Power ^ | ||
| - | | <fc #8200c8>405 (violet)</fc> | 405 nm | 15 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 15 mW | |
| - | | <fc #00f7ff>488 (blue)</fc> | 488 nm | 13 mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 13 mW | |
| - | | <fc #c6ff00>561 (yellow-green)</fc> | 561 nm | 13 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 13 mW | |
| - | | <fc #ff2600>640 (red)</fc> | 640 nm | 10 mW | | + | | Red | <fc #ff2600>640 nm</fc> | 10 mW | |
| + | |||
| + | ==== LEDs (for eyepieces) ==== | ||
| + | |||
| + | ^ Line ^ Wavelength ^ | ||
| + | | UV | <fc #be00cc>385 nm</fc> | | ||
| + | | B | <fc #00c0ff>475 nm</fc> | | ||
| + | | G | <fc #b3ff00>555 nm</fc> | | ||
| + | | R | <fc #ff4f00>630 nm</fc> | | ||
| + | |||
| + | ==== Filtersets (for eyepieces) ==== | ||
| + | |||
| + | ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | Green | 450-490 nm | 493 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/000000-1031-346|FS38]] | | ||
| + | | Orange-Red | 533-558 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | | ||
| + | | Blue | 370-410 nm | 420 nm | <fc #0046ff>430-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489096-9100-000|FS96HE]] | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| + | |||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | <fc #e94543>5x</fc> | EC Plan-Neofluar | Air | 0.16 | 18.5 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | | + | | <fc #df2b37>5x</fc> | EC Plan-Neofluar | Air | 0.16 | 18.5 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | |
| | <fc #fef042>10x</fc> | Plan-Apochromat | Air | 0.45 | 2 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | | <fc #fef042>10x</fc> | Plan-Apochromat | Air | 0.45 | 2 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | ||
| | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | ||
| | <fc #01b3f5>40x</fc> | LD LCI Plan-Apochromat | W, Sil, G | 1.2 | 0.41 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | | <fc #01b3f5>40x</fc> | LD LCI Plan-Apochromat | W, Sil, G | 1.2 | 0.41 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | ||
| | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
| - | |||
| - | ==== LEDs (Ocular) ==== | ||
| - | |||
| - | ^ Line ^ Wavelength ^ | ||
| - | | <fc #6f0077>UV</fc> | 385 nm | | ||
| - | | <fc #00c0ff>B</fc> | 475 nm | | ||
| - | | <fc #b3ff00>G</fc> | 555 nm | | ||
| - | | <fc #ff4f00>R</fc> | 630 nm | | ||
| - | |||
| - | ==== Emission Filters ==== | ||
| - | |||
| - | ^ Filter name ^ Transmission(nm) ^ Reference ^ | ||
| - | | BFP | 450/40 | [[https://www.fpbase.org/spectra/?s=2666,2668,2667,2055&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|96 HE]] | | ||
| - | | GFP | 425/50 | [[https://www.fpbase.org/spectra/?s=1484,1495,1485,2057&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|38 HE]] | | ||
| - | | Alexa 546 | LP590 | [[https://www.fpbase.org/spectra/?s=1530,1533,1536,2059&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|15]] | | ||
| - | | Cy5 | 690/50 | [[https://www.fpbase.org/spectra/?s=1547,1550,1543,2068&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|50]] | | ||
| - | | DIC | <800 | - | | ||
| - | | mPlum | 629/62 | [[https://www.fpbase.org/spectra/?s=2620,2618,2619,2069&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|64 HE]] | | ||
| - | | Quad | 425/514/592/709 | [[https://www.fpbase.org/spectra/?s=2657,2601,2602,2658,2577&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|90 HE]] | | ||
| ===== How to start your session ===== | ===== How to start your session ===== | ||
| Line 88: | Line 86: | ||
| * Turn off the power bar | * Turn off the power bar | ||
| - | {{::Oeiras_LSM900_powerbar.png?300|}} | + | {{::Oeiras_LSM900_powerbar.png?200|}} |
| * Turn off Components | * Turn off Components | ||
| * Turn off Systems | * Turn off Systems | ||
| Line 95: | Line 93: | ||
| * Turn on Components | * Turn on Components | ||
| * Turn on the incubation module (if needed - turn on 30 mins before acquiring) | * Turn on the incubation module (if needed - turn on 30 mins before acquiring) | ||
| - | {{::Oeiras_LSM900_incubation.png?300|}} | + | {{::Oeiras_LSM900_incubation.png?200|}} |
| + | * Turn on the power bar | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| * Start **ZEN** | * Start **ZEN** | ||
zeiss_lsm_900.1765214187.txt.gz · Last modified: 2025/12/08 18:16 by bioimaging
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