zeiss_lsm_900
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zeiss_lsm_900 [2025/12/08 18:05] bioimaging |
zeiss_lsm_900 [2025/12/12 14:56] (current) bioimaging [Filtersets (for eyepieces)] |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=lsm_900.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lsm_900.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=lsm_900.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lsm_900.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B03<br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_zeiss_lsm_900.png">[Oeiras] Room 0B03</a><br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html">LSM 900 with Airyscan 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html">LSM 900 with Airyscan 2</a><br> | ||
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| ^ LASER ^ Wavelength ^ Maximum Power ^ | ^ LASER ^ Wavelength ^ Maximum Power ^ | ||
| - | | <fc #8200c8>405 (violet)</fc> | 405 nm | 15 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 15 mW | |
| - | | <fc #00f7ff>488 (blue)</fc> | 488 nm | 13 mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 13 mW | |
| - | | <fc #c6ff00>561 (yellow-green)</fc> | 561 nm | 13 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 13 mW | |
| - | | <fc #ff2600>640 (red)</fc> | 640 nm | 10 mW | | + | | Red | <fc #ff2600>640 nm</fc> | 10 mW | |
| + | |||
| + | ==== LEDs (for eyepieces) ==== | ||
| + | |||
| + | ^ Line ^ Wavelength ^ | ||
| + | | UV | <fc #be00cc>385 nm</fc> | | ||
| + | | B | <fc #00c0ff>475 nm</fc> | | ||
| + | | G | <fc #b3ff00>555 nm</fc> | | ||
| + | | R | <fc #ff4f00>630 nm</fc> | | ||
| + | |||
| + | ==== Filtersets (for eyepieces) ==== | ||
| + | |||
| + | ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | Green | 450-490 nm | 493 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/000000-1031-346|FS38]] | | ||
| + | | Orange-Red | 533-558 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=000000-1114-101&o=|FS43]] | | ||
| + | | Blue | 370-410 nm | 420 nm | <fc #0046ff>430-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489096-9100-000|FS96HE]] | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| + | |||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | <fc #e94543>5x</fc> | EC Plan-Neofluar | Air | 0.16 | 18.5 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | | + | | <fc #df2b37>5x</fc> | EC Plan-Neofluar | Air | 0.16 | 18.5 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | |
| | <fc #fef042>10x</fc> | Plan-Apochromat | Air | 0.45 | 2 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | | <fc #fef042>10x</fc> | Plan-Apochromat | Air | 0.45 | 2 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | ||
| | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | | <fc #0aa05c>20x</fc> | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | ||
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| | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
| - | ==== LEDs (Ocular) ==== | + | ===== How to start your session ===== |
| - | ^ Line ^ Wavelength ^ | + | If you are the **first user** of the day: |
| - | | <fc #6f0077>UV</fc> | 385 nm | | + | |
| - | | <fc #00c0ff>B</fc> | 475 nm | | + | |
| - | | <fc #b3ff00>G</fc> | 555 nm | | + | |
| - | | <fc #ff4f00>R</fc> | 630 nm | | + | |
| - | ==== Emission Filters ==== | + | * Turn off the power bar |
| - | + | {{::Oeiras_LSM900_powerbar.png?200|}} | |
| - | ^ Filter name ^ Transmission(nm) ^ Reference ^ | + | * Turn off Components |
| - | | BFP | 450/40 | [[https://www.fpbase.org/spectra/?s=2666,2668,2667,2055&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|96 HE]] | | + | * Turn off Systems |
| - | | GFP | 425/50 | [[https://www.fpbase.org/spectra/?s=1484,1495,1485,2057&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|38 HE]] | | + | * Turn on the computer |
| - | | Alexa 546 | LP590 | [[https://www.fpbase.org/spectra/?s=1530,1533,1536,2059&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|15]] | | + | * Turn on Systems |
| - | | Cy5 | 690/50 | [[https://www.fpbase.org/spectra/?s=1547,1550,1543,2068&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|50]] | | + | * Turn on Components |
| - | | DIC | <800 | - | | + | * Turn on the incubation module (if needed - turn on 30 mins before acquiring) |
| - | | mPlum | 629/62 | [[https://www.fpbase.org/spectra/?s=2620,2618,2619,2069&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|64 HE]] | | + | {{::Oeiras_LSM900_incubation.png?200|}} |
| - | | Quad | 425/514/592/709 | [[https://www.fpbase.org/spectra/?s=2657,2601,2602,2658,2577&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|90 HE]] | | + | * Turn on the power bar |
| - | + | ||
| - | ===== How to start your session ===== | + | |
| - | + | ||
| - | The system should be always on, you only need to: | + | |
| - | * If incubation is needed (turn on 30 mins before acquiring): | + | |
| - | * Turn the powerbar off | + | |
| - | {{::Oeiras_LSM900_powerbar.png?300|}} | + | |
| - | * Turn the incubation controller on | + | |
| - | {{::Oeiras_LSM900_incubation.png?300|}} | + | |
| - | * Turn the powerbar on | + | |
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| * Start **ZEN** | * Start **ZEN** | ||
| - | |||
| - | ===== How to end your session ===== | ||
| - | |||
| - | If there is another user for this microscope in the __next hour__: | ||
| - | * Exit ZEN, leave the lasers on | ||
| - | * Log off the computer | ||
| - | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| Else: | Else: | ||
| - | * Turn off the lasers in **ZEN** | ||
| - | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| - | * Exit ZEN | ||
| - | * Log off the computer | ||
| - | |||
| - | ===== Microscope Turn On Procedure (after complete shutdown) ===== | ||
| - | |||
| - | If the system is completely off, please follow this procedure: | ||
| - | |||
| - | * Turn the Laser key | ||
| - | {{::oeiras_900_turningon1.jpg?300|}} | ||
| - | * Turn on the ''SYSTEM'' and ''COMPONENTS'' switches | ||
| - | {{::oeiras_900_turningon2.jpg?300|}} | ||
| - | * Turn on the ''Power bar'' (on the back right-hand side of the microscope) | ||
| - | {{::oeiras_lsm900_powerbar.png?300|}} | ||
| - | * Turn on the computer | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| - | * Start the **ZEN** software | + | * Start **ZEN** |
| - | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | + | ===== How to end your session ===== |
| - | + | ||
| - | Please only shutdown the system if instructed to do so by the Bioimaging staff. | + | |
| - | * Turn off the lasers in **ZEN** | + | If there is another user: |
| - | * Exit ZEN | + | * Clean up immersion objectives |
| - | * Shutdown the computer | + | * Exit the ZEN software |
| - | * Turn off the ''Power Bar'' (on the back right-hand side of the microscope) | + | * Log off the computer |
| - | * Turn off the ''SYSTEM'' and ''COMPONENTS'' switches | + | * Turn off incubation module (if used) |
| - | * Turn off the Laser key | + | If you are the **last user** of the day: |
| + | * Clean up immersion objectives | ||
| + | * Exit the ZEN software | ||
| + | * Turn off the computer | ||
| + | * Turn off incubation module (if used) | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_900.1765213525.txt.gz · Last modified: 2025/12/08 18:05 by bioimaging
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