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zeiss_lsm_900 [2025/07/19 22:22] bioimaging |
zeiss_lsm_900 [2025/07/31 12:41] (current) bioimaging |
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The system should be always on, you only need to: | The system should be always on, you only need to: | ||
- | * Turn on the computer (if needed) | + | * If incubation is needed (turn on 30 mins before acquiring): |
+ | * Turn the powerbar off | ||
+ | {{::Oeiras_LSM900_powerbar.png?300|}} | ||
+ | * Turn the incubation controller on | ||
+ | {{::Oeiras_LSM900_incubation.png?300|}} | ||
+ | * Turn the powerbar on | ||
* Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
* Start **ZEN** | * Start **ZEN** | ||
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If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
- | * Exit the ZEN software, leave the lasers on | + | * Exit ZEN, leave the lasers on |
* Log off the computer | * Log off the computer | ||
* Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
Else: | Else: | ||
- | * Turn off the lasers on **ZEN** | + | * Turn off the lasers in **ZEN** |
* Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
- | * Exit the ZEN software | + | * Exit ZEN |
* Log off the computer | * Log off the computer | ||
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If the system is completely off, please follow this procedure: | If the system is completely off, please follow this procedure: | ||
- | * Turn on the ''MAIN SWITCH'' | + | * Turn the Laser key |
- | * Turn on the Laser key | + | |
{{::oeiras_900_turningon1.jpg?300|}} | {{::oeiras_900_turningon1.jpg?300|}} | ||
* Turn on the ''SYSTEM'' and ''COMPONENTS'' switches | * Turn on the ''SYSTEM'' and ''COMPONENTS'' switches | ||
{{::oeiras_900_turningon2.jpg?300|}} | {{::oeiras_900_turningon2.jpg?300|}} | ||
- | * Turn on the ''Power bar'' | + | * Turn on the ''Power bar'' (on the back right-hand side of the microscope) |
- | {{::oeiras_900_turningon3.jpg?300|}} | + | {{::oeiras_lsm900_powerbar.png?300|}} |
* Turn on the computer | * Turn on the computer | ||
* Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
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Please only shutdown the system if instructed to do so by the Bioimaging staff. | Please only shutdown the system if instructed to do so by the Bioimaging staff. | ||
- | * Turn off the lasers on **ZEN** | + | * Turn off the lasers in **ZEN** |
- | * Exit the ZEN software | + | * Exit ZEN |
* Shutdown the computer | * Shutdown the computer | ||
- | * Turn off the ''Power Bar'' | + | * Turn off the ''Power Bar'' (on the back right-hand side of the microscope) |
* Turn off the ''SYSTEM'' and ''COMPONENTS'' switches | * Turn off the ''SYSTEM'' and ''COMPONENTS'' switches | ||
* Turn off the Laser key | * Turn off the Laser key | ||
- | * Turn off the ''MAIN SWITCH'' | ||
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[[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] |