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zeiss_lsm_900 [2025/07/19 22:22] bioimaging |
zeiss_lsm_900 [2025/07/31 12:41] (current) bioimaging |
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| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html">LSM 900 with Airyscan 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html">LSM 900 with Airyscan 2</a><br> | ||
| <b>Nickname</b>: "900"<br> | <b>Nickname</b>: "900"<br> | ||
| - | <b>Software</b>: Zen 3.12<br> | + | <b>Software</b>: ZEN 3.12<br> |
| <b>Year</b>: 2022<br> | <b>Year</b>: 2022<br> | ||
| <b>SN</b>: 2901000180<br> | <b>SN</b>: 2901000180<br> | ||
| Line 88: | Line 88: | ||
| The system should be always on, you only need to: | The system should be always on, you only need to: | ||
| - | * Turn on the computer (if needed) | + | * If incubation is needed (turn on 30 mins before acquiring): |
| + | * Turn the powerbar off | ||
| + | {{::Oeiras_LSM900_powerbar.png?300|}} | ||
| + | * Turn the incubation controller on | ||
| + | {{::Oeiras_LSM900_incubation.png?300|}} | ||
| + | * Turn the powerbar on | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| * Start **ZEN** | * Start **ZEN** | ||
| Line 95: | Line 100: | ||
| If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
| - | * Exit the ZEN software, leave the lasers on | + | * Exit ZEN, leave the lasers on |
| * Log off the computer | * Log off the computer | ||
| * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| Else: | Else: | ||
| - | * Turn off the lasers on **ZEN** | + | * Turn off the lasers in **ZEN** |
| * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| - | * Exit the ZEN software | + | * Exit ZEN |
| * Log off the computer | * Log off the computer | ||
| Line 108: | Line 113: | ||
| If the system is completely off, please follow this procedure: | If the system is completely off, please follow this procedure: | ||
| - | * Turn on the ''MAIN SWITCH'' | + | * Turn the Laser key |
| - | * Turn on the Laser key | + | |
| {{::oeiras_900_turningon1.jpg?300|}} | {{::oeiras_900_turningon1.jpg?300|}} | ||
| * Turn on the ''SYSTEM'' and ''COMPONENTS'' switches | * Turn on the ''SYSTEM'' and ''COMPONENTS'' switches | ||
| {{::oeiras_900_turningon2.jpg?300|}} | {{::oeiras_900_turningon2.jpg?300|}} | ||
| - | * Turn on the ''Power bar'' | + | * Turn on the ''Power bar'' (on the back right-hand side of the microscope) |
| - | {{::oeiras_900_turningon3.jpg?300|}} | + | {{::oeiras_lsm900_powerbar.png?300|}} |
| * Turn on the computer | * Turn on the computer | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
| Line 123: | Line 127: | ||
| Please only shutdown the system if instructed to do so by the Bioimaging staff. | Please only shutdown the system if instructed to do so by the Bioimaging staff. | ||
| - | * Turn off the lasers on **ZEN** | + | * Turn off the lasers in **ZEN** |
| - | * Exit the ZEN software | + | * Exit ZEN |
| * Shutdown the computer | * Shutdown the computer | ||
| - | * Turn off the ''Power Bar'' | + | * Turn off the ''Power Bar'' (on the back right-hand side of the microscope) |
| * Turn off the ''SYSTEM'' and ''COMPONENTS'' switches | * Turn off the ''SYSTEM'' and ''COMPONENTS'' switches | ||
| * Turn off the Laser key | * Turn off the Laser key | ||
| - | * Turn off the ''MAIN SWITCH'' | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||