zeiss_lsm_900
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zeiss_lsm_900 [2025/01/13 10:36] bioimaging [Microscope Turn On Procedure] |
zeiss_lsm_900 [2025/07/31 12:41] (current) bioimaging |
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| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html">LSM 900 with Airyscan 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html">LSM 900 with Airyscan 2</a><br> | ||
| <b>Nickname</b>: "900"<br> | <b>Nickname</b>: "900"<br> | ||
| - | <b>Software</b>: Zen 3.5<br> | + | <b>Software</b>: ZEN 3.12<br> |
| <b>Year</b>: 2022<br> | <b>Year</b>: 2022<br> | ||
| - | <b>SN</b>: <br> | + | <b>SN</b>: 2901000180<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
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| * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]] | * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]] | ||
| * **Features** | * **Features** | ||
| - | * | [[https://www.imdik.pan.pl/images/Us%C5%82ugi_dokumenty/Przydatne_informacje/2d_Definite_Focus_information.pdf|Definite Focus.3]] | + | * | [[https://www.imdik.pan.pl/images/Us%C5%82ugi_dokumenty/Przydatne_informacje/2d_Definite_Focus_information.pdf|Definite Focus.3]] |
| * | [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Airyscan 2 Multiplex]] | * | [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Airyscan 2 Multiplex]] | ||
| * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus]] | * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus]] | ||
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| <html> | <html> | ||
| - | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/watch?v=odXtaRLHhIQ" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> | + | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/odXtaRLHhIQ" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> |
| </html> | </html> | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
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| - | ==== Filterset ==== | + | ==== Emission Filters ==== |
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ^ Filter name ^ Transmission(nm) ^ Reference ^ |
| - | | | | | | | | | + | | BFP | 450/40 | [[https://www.fpbase.org/spectra/?s=2666,2668,2667,2055&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|96 HE]] | |
| - | ===== Microscope Turn On Procedure ===== | + | | GFP | 425/50 | [[https://www.fpbase.org/spectra/?s=1484,1495,1485,2057&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|38 HE]] | |
| + | | Alexa 546 | LP590 | [[https://www.fpbase.org/spectra/?s=1530,1533,1536,2059&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|15]] | | ||
| + | | Cy5 | 690/50 | [[https://www.fpbase.org/spectra/?s=1547,1550,1543,2068&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|50]] | | ||
| + | | DIC | <800 | - | | ||
| + | | mPlum | 629/62 | [[https://www.fpbase.org/spectra/?s=2620,2618,2619,2069&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|64 HE]] | | ||
| + | | Quad | 425/514/592/709 | [[https://www.fpbase.org/spectra/?s=2657,2601,2602,2658,2577&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|90 HE]] | | ||
| - | * Turn on the ''Laser Key'' | + | ===== How to start your session ===== |
| + | |||
| + | The system should be always on, you only need to: | ||
| + | * If incubation is needed (turn on 30 mins before acquiring): | ||
| + | * Turn the powerbar off | ||
| + | {{::Oeiras_LSM900_powerbar.png?300|}} | ||
| + | * Turn the incubation controller on | ||
| + | {{::Oeiras_LSM900_incubation.png?300|}} | ||
| + | * Turn the powerbar on | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start **ZEN** | ||
| + | |||
| + | ===== How to end your session ===== | ||
| + | |||
| + | If there is another user for this microscope in the __next hour__: | ||
| + | * Exit ZEN, leave the lasers on | ||
| + | * Log off the computer | ||
| + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| + | Else: | ||
| + | * Turn off the lasers in **ZEN** | ||
| + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| + | * Exit ZEN | ||
| + | * Log off the computer | ||
| + | |||
| + | ===== Microscope Turn On Procedure (after complete shutdown) ===== | ||
| + | |||
| + | If the system is completely off, please follow this procedure: | ||
| + | |||
| + | * Turn the Laser key | ||
| {{::oeiras_900_turningon1.jpg?300|}} | {{::oeiras_900_turningon1.jpg?300|}} | ||
| - | * Turn on the ''System and Components'' switch | + | * Turn on the ''SYSTEM'' and ''COMPONENTS'' switches |
| {{::oeiras_900_turningon2.jpg?300|}} | {{::oeiras_900_turningon2.jpg?300|}} | ||
| - | * Turn on the ''Power bar'' | + | * Turn on the ''Power bar'' (on the back right-hand side of the microscope) |
| - | {{::oeiras_900_turningon3.jpg?300|}} | + | {{::oeiras_lsm900_powerbar.png?300|}} |
| * Turn on the computer | * Turn on the computer | ||
| * Log into Windows with your Agendo credentials | * Log into Windows with your Agendo credentials | ||
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| ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ||
| - | Only shutdown te system if there is no one else booked for the next two hours | + | Please only shutdown the system if instructed to do so by the Bioimaging staff. |
| - | * Exit the ZEN software | + | * Turn off the lasers in **ZEN** |
| + | * Exit ZEN | ||
| * Shutdown the computer | * Shutdown the computer | ||
| - | * Turn off the ''Power Bar'' | + | * Turn off the ''Power Bar'' (on the back right-hand side of the microscope) |
| - | * Turn off the ''System and Components'' switch | + | * Turn off the ''SYSTEM'' and ''COMPONENTS'' switches |
| - | * Turn off the ''Laser Key'' | + | * Turn off the Laser key |
| ---- | ---- | ||
| + | |||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_900.1736760973.txt.gz · Last modified: 2025/01/13 10:36 by bioimaging
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