Differences

This shows you the differences between two versions of the page.

--- zeiss_lsm_900 [2025/01/13 10:28]
bioimaging [Microscope Turn On Procedure]
+++ zeiss_lsm_900 [2025/07/31 12:41] (current)
bioimaging
@@ Line 10: / Line 10: @@
 <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/confocal-microscopes/lsm-900-with-airyscan-2.html">LSM 900 with Airyscan 2</a><br>
 <b>Nickname</b>: "900"<br>
-<b>Software</b>: Zen 3.5<br>
+<b>Software</b>: ZEN 3.12<br>
 <b>Year</b>:  2022<br>
-<b>SN</b>:  <br>
+<b>SN</b>: 2901000180<br>
 &nbsp <br>
 Data will be deleted after: <b>1 month</b>
@@ Line 33: / Line 33: @@
   * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]]
   * **Features**
-    * |  [[https://www.imdik.pan.pl/images/Us%C5%82ugi_dokumenty/Przydatne_informacje/2d_Definite_Focus_information.pdf|Definite Focus.3]]
+    * | [[https://www.imdik.pan.pl/images/Us%C5%82ugi_dokumenty/Przydatne_informacje/2d_Definite_Focus_information.pdf|Definite Focus.3]]
     * | [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Airyscan 2 Multiplex]]
     * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus]]
@@ Line 39: / Line 39: @@
 <html>
-<p align="center"><iframe width="420" height="236" src="https://www.youtube.com/watch?v=odXtaRLHhIQ" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p>
+<p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/odXtaRLHhIQ" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p>
 </html>
-{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
+{{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.
@@ Line 74: / Line 74: @@
-==== Filterset ====
+==== Emission Filters ====
-^  Position  ^  Filterset  ^  Reference  ^  Excitation  ^  Dichroic  ^  Emission  ^
+^  Filter name  ^  Transmission(nm)  ^  Reference  ^
-|    |    |  |  |  |  |
+|  BFP  |  450/40  | [[https://www.fpbase.org/spectra/?s=2666,2668,2667,2055&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|96 HE]] |
-===== Microscope Turn On Procedure =====
+|  GFP  |  425/50  | [[https://www.fpbase.org/spectra/?s=1484,1495,1485,2057&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|38 HE]] |
+|  Alexa 546  |  LP590  | [[https://www.fpbase.org/spectra/?s=1530,1533,1536,2059&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|15]] |
+|  Cy5  |  690/50  | [[https://www.fpbase.org/spectra/?s=1547,1550,1543,2068&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|50]] |
+|  DIC  |  <800  | - |
+|  mPlum  |  629/62  | [[https://www.fpbase.org/spectra/?s=2620,2618,2619,2069&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|64 HE]] |
+|  Quad  |  425/514/592/709  | [[https://www.fpbase.org/spectra/?s=2657,2601,2602,2658,2577&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|90 HE]] |
-  * Turn on the ''Laser Key''
+===== How to start your session =====
+The system should be always on, you only need to:
+  * If incubation is needed (turn on 30 mins before acquiring):
+    * Turn the powerbar off
+{{::Oeiras_LSM900_powerbar.png?300|}}
+    * Turn the incubation controller on
+{{::Oeiras_LSM900_incubation.png?300|}}
+    * Turn the powerbar on
+  * Log into Windows with your Agendo credentials
+  * Start **ZEN**
+===== How to end your session =====
+If there is another user for this microscope in the __next hour__:
+  * Exit ZEN, leave the lasers on
+  * Log off the computer
+  * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper)
+Else:
+  * Turn off the lasers in **ZEN**
+  * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper)
+  * Exit ZEN
+  * Log off the computer
+===== Microscope Turn On Procedure (after complete shutdown) =====
+If the system is completely off, please follow this procedure:
+  * Turn the Laser key
 {{::oeiras_900_turningon1.jpg?300|}}
-  * Turn on the ''System and Components'' switch
+  * Turn on the ''SYSTEM'' and ''COMPONENTS'' switches
 {{::oeiras_900_turningon2.jpg?300|}}
-  * Turn on the ''Power bar''
+  * Turn on the ''Power bar'' (on the back right-hand side of the microscope)
-{{::oeiras_900_turningon3.jpg?300|}}
+{{::oeiras_lsm900_powerbar.png?300|}}
   * Turn on the computer
   * Log into Windows with your Agendo credentials
   * Start the **ZEN** software
+===== Microscope Turn Off Procedure (for complete shutdown) =====
+Please only shutdown the system if instructed to do so by the Bioimaging staff.
+  * Turn off the lasers in **ZEN**
+  * Exit ZEN
+  * Shutdown the computer
+  * Turn off the ''Power Bar'' (on the back right-hand side of the microscope)
+  * Turn off the ''SYSTEM'' and ''COMPONENTS'' switches
+  * Turn off the Laser key
 ----
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