zeiss_lsm_900
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zeiss_lsm_900 [2025/01/08 17:15] bioimaging [Microscope overview] |
zeiss_lsm_900 [2025/01/13 16:21] (current) bioimaging [Microscope overview] |
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| * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]] | * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 7]] | ||
| * **Features** | * **Features** | ||
| - | * | [[https://www.imdik.pan.pl/images/Us%C5%82ugi_dokumenty/Przydatne_informacje/2d_Definite_Focus_information.pdf|Definite Focus.3]] | + | * | [[https://www.imdik.pan.pl/images/Us%C5%82ugi_dokumenty/Przydatne_informacje/2d_Definite_Focus_information.pdf|Definite Focus.3]] |
| * | [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Airyscan 2 Multiplex]] | * | [[https://www.youtube.com/watch?v=tUiEa7vcggQ|Airyscan 2 Multiplex]] | ||
| * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus]] | * | [[https://www.zeiss.com/microscopy/en/about-us/newsroom/press-releases/2021/zeiss-lsm-plus-and-zeiss-airyscan-joint-deconvolution.html|LSM Plus]] | ||
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| <html> | <html> | ||
| - | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/watch?v=odXtaRLHhIQ" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> | + | <p align="center"><iframe width="420" height="236" src="https://www.youtube.com/embed/odXtaRLHhIQ" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe></p> |
| </html> | </html> | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== System components ===== | ===== System components ===== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ==== LASERs ==== |
| - | | | | | | | | | + | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ |
| + | | 405 | 405 nm | 15 mW | | ||
| + | | 488 | 488 nm | 13 mW | | ||
| + | | 561 | 561 nm | 13 mW | | ||
| + | | 640 | 640 nm | 10 mW | | ||
| + | |||
| + | ==== LEDs (Ocular) ==== | ||
| + | ^ Line ^ Wavelength ^ | ||
| + | | UV | 385 nm | | ||
| + | | B | 475 nm | | ||
| + | | G | 555 nm | | ||
| + | | R | 630 nm | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ |
| - | | | | | | | | + | | 5x | EC Plan-Neofluar | Air | 0.16 | 18.5 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | |
| + | | 10x | Plan-Apochromat | Air | 0.45 | 2 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420640-9900-000/Objective-Plan-Apochromat-10x-0.45-M27|420640-9900-000]] | | ||
| + | | 20x | Plan-Apochromat | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420650-9903-000/Objective-Plan-Apochromat-20x-0.8-M27|420650-9903-000]] | | ||
| + | | 40x | LD LCI Plan-Apochromat | W, Sil, G | 1.2 | 0.41 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420862-9970-799/Objective-LD-LCI-Plan-Apochromat-40x-1.2-Imm-Corr-DIC-M27|420862-9970-799]] | | ||
| + | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/420782-9900-799/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-799]] | | ||
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| + | ==== Emission Filters ==== | ||
| + | |||
| + | ^ Filter name ^ Transmission(nm) ^ Reference ^ | ||
| + | | BFP | 450/40 | [[https://www.fpbase.org/spectra/?s=2666,2668,2667,2055&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|96 HE]] | | ||
| + | | GFP | 425/50 | [[https://www.fpbase.org/spectra/?s=1484,1495,1485,2057&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|38 HE]] | | ||
| + | | Alexa 546 | LP590 | [[https://www.fpbase.org/spectra/?s=1530,1533,1536,2059&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|15]] | | ||
| + | | Cy5 | 690/50 | [[https://www.fpbase.org/spectra/?s=1547,1550,1543,2068&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|50]] | | ||
| + | | DIC | <800 | - | | ||
| + | | mPlum | 629/62 | [[https://www.fpbase.org/spectra/?s=2620,2618,2619,2069&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|64 HE]] | | ||
| + | | Quad | 425/514/592/709 | [[https://www.fpbase.org/spectra/?s=2657,2601,2602,2658,2577&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|90 HE]] | | ||
| + | ===== Microscope Turn On Procedure ===== | ||
| + | |||
| + | * Turn on the ''Laser Key'' | ||
| + | {{::oeiras_900_turningon1.jpg?300|}} | ||
| + | * Turn on the ''System and Components'' switch | ||
| + | {{::oeiras_900_turningon2.jpg?300|}} | ||
| + | * Turn on the ''Power bar'' | ||
| + | {{::oeiras_900_turningon3.jpg?300|}} | ||
| + | * Turn on the computer | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start the **ZEN** software | ||
| - | ==== Camera ==== | + | ===== Microscope Turn Off Procedure (for complete shutdown) ===== |
| + | Only shutdown te system if there is no one else booked for the next two hours | ||
| + | * Exit the ZEN software | ||
| + | * Shutdown the computer | ||
| + | * Turn off the ''Power Bar'' | ||
| + | * Turn off the ''System and Components'' switch | ||
| + | * Turn off the ''Laser Key'' | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lsm_900.1736352948.txt.gz · Last modified: 2025/01/08 17:15 by bioimaging
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