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zeiss_lsm_510 [2016/04/17 01:24] bioimaging [Zeiss LSM 510 (no longer available)] |
zeiss_lsm_510 [2023/08/06 13:34] (current) bioimaging |
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| <html> | <html> | ||
| <UL> | <UL> | ||
| - | <a href="http://www.imm.fm.ul.pt/booking/month.php?user=ZEISSLSM51&rid=2"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss LSM 510 Booking</a> | + | <a href="http://www.imm.fm.ul.pt/booking/old/month.php?user=ZEISSLSM51&rid=2"><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss LSM 510 Booking</a> |
| </UL> | </UL> | ||
| <UL> | <UL> | ||
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| </html> | </html> | ||
| - | **Location**: Room P3-A-10 <html>(<img src="http://www.imm.fm.ul.pt/wiki/data/media/bioimaging/telephone_icon.gif" width=15> 47311)<BR></html> | + | **Location**: no longer available <html><BR></html> |
| **Manufacturer**: Zeiss <html><BR></html> | **Manufacturer**: Zeiss <html><BR></html> | ||
| **Model**: LSM 510 <html><BR></html> | **Model**: LSM 510 <html><BR></html> | ||
| Line 33: | Line 33: | ||
| ==== Filter and Dichroic sets ==== | ==== Filter and Dichroic sets ==== | ||
| - | | {{:bioimaging:510_main.jpg?120|}} | {{:bioimaging:510_emmission1.jpg?120|}} | {{:bioimaging:510_emmission2.jpg?120|}} | {{:bioimaging:510_2dichroic.jpg?120|}} | {{:bioimaging:510_1dichroic.jpg?120|}} | | + | | {{510_main.jpg?120|}} | {{510_emmission1.jpg?120|}} | {{510_emmission2.jpg?120|}} | {{510_2dichroic.jpg?120|}} | {{510_1dichroic.jpg?120|}} | |
| | Main configuration window | Emission filters for channel 1 | Emission filters for channel 2 | Channel separation dichroic | Laser separation dichroic | | | Main configuration window | Emission filters for channel 1 | Emission filters for channel 2 | Channel separation dichroic | Laser separation dichroic | | ||
| Line 39: | Line 39: | ||
| ===== Microscope Turn On Procedures ===== | ===== Microscope Turn On Procedures ===== | ||
| * Turn on the two UPS near the microscope. | * Turn on the two UPS near the microscope. | ||
| - | {{the_microscopy_wiki:zeiss_lsm510_ups.jpg?100}} | + | {{zeiss_lsm510_ups.jpg?100}} |
| * Turn the remote control on. | * Turn the remote control on. | ||
| - | {{the_microscopy_wiki:zeiss_lsm510_remote_control.jpg?100}} | + | {{zeiss_lsm510_remote_control.jpg?100}} |
| * Turn the HBO lamp on. | * Turn the HBO lamp on. | ||
| - | {{the_microscopy_wiki:zeiss_lsm510_fluorescence.jpg?100}} | + | {{zeiss_lsm510_fluorescence.jpg?100}} |
| * Turn the halogen transmitted light power source on. | * Turn the halogen transmitted light power source on. | ||
| - | {{the_microscopy_wiki:zeiss_lsm510_transmited.jpg?100}} | + | {{zeiss_lsm510_transmited.jpg?100}} |
| * Turn the computer on. | * Turn the computer on. | ||
| * Login to the computer, start the LSM software. | * Login to the computer, start the LSM software. | ||
| Line 52: | Line 52: | ||
| If there's another user for this microscope in the next two hours: | If there's another user for this microscope in the next two hours: | ||
| * Close the software, leave the lasers on an log off the computer. | * Close the software, leave the lasers on an log off the computer. | ||
| - | * Clean up any obejctive were you used immersion oil. | + | * Clean up any objective were you used immersion oil. |
| - | * Make sure that there realy is another user going to use the microscope. | + | * Make sure that there really is another user going to use the microscope. |
| Else: | Else: | ||
| * Clean up any objective were you used immersion oil. | * Clean up any objective were you used immersion oil. | ||
| Line 67: | Line 67: | ||
| ===== External links ===== | ===== External links ===== | ||
| - | * This {{bioimaging:lsm510.pdf|PDF}} shows how to work with the system, it's not exactly the same system configuration but teaches a lot about the software. | + | * This {{lsm510.pdf|PDF}} shows how to work with the system, it's not exactly the same system configuration but teaches a lot about the software. |
| - | + | ||
| - | + | ||
| - | ===== TOP 5 Users ===== | + | |
| - | ^ 2008 ^^ 2007 ^^ | + | |
| - | | Marco Antunes (UMO) | 123.5h | Jennifer Geiger (UMO) | 339.5h | | + | |
| - | | Anna Zaidman (UMO) | 90.5h | Isabel Campos (UMO) | 193h | | + | |
| - | | Jennifer Geiger (UMO) | 65h | Margarida Silva (UBD) | 130h | | + | |
| - | | Ana Santos (UMO) | 64.5h | Marco Antunes (UMO) | 115h | | + | |
| - | | Mariana Simões (UMO) | 64h | Ana Santos (UMO) | 99h | | + | |
| ---- | ---- | ||
| - | [[groups:bioimage:resources|Back to the Resources page]] | + | [[resources|Back to the Equipment page]] |