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zeiss_lightsheet_z1 [2024/07/24 00:37] bioimaging |
zeiss_lightsheet_z1 [2026/02/17 17:28] (current) bioimaging [Filtersets (Water)] |
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| <html> | <html> | ||
| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_zeiss_lightsheet_z.1_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_map_new_p2a24.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_map_new_p2a24.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lightsheet Z.1 Usage Statistics</a> | + | → <a href="/facility/bioimaging/doku.php?id=zeiss_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lightsheet Z.1 Usage Statistics</a> |
| </p> | </p> | ||
| </td> | </td> | ||
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| {{:info.png?25|}} Click on the image on the right to see the system beam path in higher detail | {{:info.png?25|}} Click on the image on the right to see the system beam path in higher detail | ||
| - | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== Additional information for sample preparation ===== | ===== Additional information for sample preparation ===== | ||
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| ==== LASERs ==== | ==== LASERs ==== | ||
| + | |||
| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | Solid State 488 | 488 nm | 30 mW | | + | | Blue | <fc #00f7ff>488</fc> | 30 mW | |
| - | | Solid State 561 | 561 nm | 20 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 20 mW | |
| - | | Solid State 638 | 638 nm | 75 mW | | + | | Red | <fc #ff4200>638 nm</fc> | 75 mW | |
| ==== Objectives (Illumination) ==== | ==== Objectives (Illumination) ==== | ||
| ^ Magnification ^ Model ^ NA ^ | ^ Magnification ^ Model ^ NA ^ | ||
| - | | 10x | Lightsheet Z.1 10x | 0.20 | | + | | <fc #fef042>10x</fc> | Lightsheet Z.1 10x | 0.20 | |
| ==== Objectives (Detection) ==== | ==== Objectives (Detection) ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 20x | W Plan-Apochromat DIC 75mm | Water | 1.00 | 2.4 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421452-9700-000/Objective-W-Plan-Apochromat-20x-1.0-DIC-M27-75mm#|421452-9700-000]] | | + | | <fc #0aa05c>20x</fc> | W Plan-Apochromat DIC 75mm | Water | 1.00 | 2.4 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421452-9700-000/Objective-W-Plan-Apochromat-20x-1.0-DIC-M27-75mm#|421452-9700-000]] | |
| - | | 20x | Clr Plan-Neofluar Corr nd=1.45 85mm | Clearing | 1.00 | 5.6 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421459-9970-000/Objective-Clr-Plan-Neofluar-20x-1.0-Corr-nd-1.45-M32-85mm|421459-9970-000]] | | + | | <fc #0aa05c>20x</fc> | Clr Plan-Neofluar Corr nd=1.45 85mm | Clearing | 1.00 | 5.6 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421459-9970-000/Objective-Clr-Plan-Neofluar-20x-1.0-Corr-nd-1.45-M32-85mm|421459-9970-000]] | |
| ==== Filtersets (Water) ==== | ==== Filtersets (Water) ==== | ||
| ^ Filterset ^ Emission ^ | ^ Filterset ^ Emission ^ | ||
| - | | GFP | 505-545 nm | | + | | GFP | <fc #4aff00>505-545 nm</fc> | |
| - | | Cy3 | 575-615 nm | | + | | Cy3 | <fc #ffcf00>575-615 nm</fc> | |
| - | | DRAQ5 | LP 660 nm | | + | | DRAQ5 | <fc #ff0000>> 660 nm</fc> | |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_lightsheet_z1.1721774224.txt.gz · Last modified: 2024/07/24 00:37 by bioimaging
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