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zeiss_lightsheet_z1

Location: Room P3-A-3
Manufacturer: Carl Zeiss MicroImaging
Model: Lightsheet Z.1
Nickname: "Lightsheet"
Software: ZEN 2014 SP1
Year: 2016
SN: 2583000193
 
 
 
 
Zeiss Lightsheet Z1 Booking
Zeiss Lightsheet Z1 Usage Statistics

Microscope overview

The Zeiss Lightsheet Z1 is a light sheet fluorescence microscope able to image optical sections of large samples at subcellular resolution and very fast rates, with almost no phototoxicity or bleaching. It splits fluorescence excitation and detection into two separate light paths, with the axis of illumination being perpendicular to the detection axis. Since only a single thin section of the sample is illuminated by the light sheet, optical sectioning is achieved without a pinhole or image processing deconvolution. Light from the in-focus plane is collected by a sCMOS camera rather than pixel by pixel as in a point scanning laser confocal. This allows you to collect images faster and with less excitation light than you would with many other optical-sectioning microscopy techniques. You can image living samples in water or fixed samples where tissue clearing has been performed. Samples are mounted not on coverslips but inside special chambers that provide heating and cooling. The system is equipped with lasers from green to far red (488, 561 and 638 nm excitation wavelenghts) and appropriate fluorescence emission filters. With this system you can image live drosophila and zebrafish samples or use tissue clearing to perform optical sectioning deep into large fluorescent samples such as tissue sections, brains, embryos, organs, spheroids or biopsies. If you need higher resolution and have a smaller and thinner sample, check instead a confocal microscope such as the spinning disk 3i Marianas SDC, the line scanning Zeiss LSM 7 Live or the point scanners Zeiss LSM 880 and Zeiss LSM 710. A dedicated computer running arivis Vision4D is available for working with multi-channel 2D, 3D and 4D images you generate in the system.

Click on the image on the left to see the system beam path in higher detail

Additional information for sample preparation

System components

LASERs

Laser Unit Wavelength Maximum Power Current Status
Solid State 488 488 nm 30 mW ok
Solid State 561 561 nm 20 mW ok
Solid State 638 638 nm 75 mW ok

Objectives (Illumination)

Magnification Model NA
10x Lightsheet Z.1 10x 0.20

Objectives (Detection)

Magnification Model Type NA WD (mm)
20x W Plan-Apochromat Water 1.00 2.4
20x Clr Plan-Neofluar Corr nd=1.45 Clearing 1.00 5.6

Filtersets (Water)

Filterset Emission
GFP 505-545 nm
Cy3 575-615 nm
DRAQ5 LP 660 nm

If you want to use the system:

  • Please contact the Bioimaging Facility (Ext: 47316). Sample chamber mounting and system initialization is performed by our staff.

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zeiss_lightsheet_z1.txt ยท Last modified: 2017/07/13 16:24 by bioimaging