zeiss_celldiscoverer_7
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zeiss_celldiscoverer_7 [2025/12/09 16:59] bioimaging [Filtersets (widefield)] |
zeiss_celldiscoverer_7 [2025/12/11 23:47] (current) bioimaging [LASERs (confocal)] |
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| - | * [[https://www.zeiss.com/microscopy/en/resources/insights-hub/life-sciences/the-smart-microscope-for-automated-workflows-celldiscoverer-7-with-lsm-900.html|ZEISS Webinar: LSM 980 Airyscan 2 Multiplex mode]] | + | * [[https://www.zeiss.com/microscopy/en/resources/insights-hub/life-sciences/the-smart-microscope-for-automated-workflows-celldiscoverer-7-with-lsm-900.html|ZEISS Webinar: The Smart Microscope for Automated Workflows - Celldiscoverer 7 with LSM 900]] |
| {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | ||
| + | ===== Robotic Loader Integration ===== | ||
| + | |||
| + | <html> | ||
| + | <table style="border:0px solid white;"> | ||
| + | <tr style="border:0px solid white;"> | ||
| + | <td width="225" height="400" style="border:0px solid white;"> | ||
| + | <iframe width="225" height="400" src="https://www.youtube.com/embed/72Hi3Ug6_0k?autoplay=0&mute=1&modestbranding=1&showinfo=0&controls=1;rel=0;playsinline=1" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe> | ||
| + | </td> | ||
| + | <td style="border:0px solid white;"> | ||
| + | The ZEISS Celldiscoverer 7 integrates with an <b>advanced robotic loader</b>, <b>automated liquid handler</b>, and <b>incubator</b> to enable high-throughput, fully automated workflows for live-cell imaging. This integration allows for automated plate loading, liquid handling, and imaging of multiple plates stored under controlled conditions in the incubator, reducing manual intervention and simplifying complex experimental protocols. The loader supports <a href="https://theautomatedlab.com/article.html?content=microplates#:~:text=Standardization:%20The%20SBS%20Footprint,on%20a%20predictable%20plate%20footprint">all SBS multi-well plate formats</a>. The liquid handler includes a custom HEPA downflow to prevent contamination during sample preparation for cell-based screening assays.<br> | ||
| + | <br> | ||
| + | • Robotic loader: <a href="https://paa-automation.com/product/kx2-500/">Peak Analysis & Automation KX2-500</a><br> | ||
| + | • Automated incubator: <a href="https://www.thermofisher.com/order/catalog/product/51032950">Thermo Fisher Cytomat 2 C</a><br> | ||
| + | • Automated Liquid Handler: <a href="https://www.agilent.com/en/product/automated-liquid-handling/automated-liquid-handling-platforms/bravo-automated-liquid-handling-platform">Agilent Bravo</a><br> | ||
| + | <br> | ||
| + | <a href="https://paa-automation.com/product/kx2-500/"><img src="/facility/bioimaging/lib/exe/fetch.php?media=kx_2-500.png" width=118></a><a href="https://www.thermofisher.com/order/catalog/product/51032950"><img src="/facility/bioimaging/lib/exe/fetch.php?media=cytomat.png" width=137></a><a href="https://www.agilent.com/en/product/automated-liquid-handling/automated-liquid-handling-platforms/bravo-automated-liquid-handling-platform"><img src="/facility/bioimaging/lib/exe/fetch.php?media=bravo.png" width=137></a> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </html> | ||
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| ^ LASER ^ Wavelength ^ Maximum Power ^ | ^ LASER ^ Wavelength ^ Maximum Power ^ | ||
| - | | violet | <fc #a600ff>405 nm</fc> | 5 mW | | + | | Violet | <fc #a600ff>405 nm</fc> | 5 mW | |
| - | | blue | <fc #00f7ff>488 nm</fc> | 10 mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 10 mW | |
| - | | yellow-green | <fc #c6ff00>561 nm</fc> | 10 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 10 mW | |
| - | | red | <fc #ff2100>640 nm</fc> | 5 mW | | + | | Red | <fc #ff2100>640 nm</fc> | 5 mW | |
| ==== LEDs (widefield) ==== | ==== LEDs (widefield) ==== | ||
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| ==== Filtersets (widefield) ==== | ==== Filtersets (widefield) ==== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Dichroic ^ Emission ^ | + | ^ Position ^ Filterset ^ Dichroic ^ Emission ^ Reference ^ |
| - | | 1 | RQBS | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000|FS90HE]] | <fc #a600ff>405 nm</fc> | <fc #5400ff>410-440 nm</fc> | | + | | 1 | RQBS | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000|FS90HE]] | |
| - | | ::: | ::: | ::: | <fc #00ffe0>493 nm</fc> | <fc #1fff00>499-529 nm</fc> | | + | | ::: | ::: | 493 nm | <fc #1fff00>499-529 nm</fc> | ::: | |
| - | | ::: | ::: | ::: | <fc #f0ff00>575 nm</fc> | <fc #ffd900>580-605 nm</fc> | | + | | ::: | ::: | 575 nm | <fc #ffd900>580-605 nm</fc> | ::: | |
| - | | ::: | ::: | ::: | <fc #ff0000>653 nm</fc> | <fc #ef0000>659-759 nm</fc> | | + | | ::: | ::: | 653 nm | <fc #ef0000>659-759 nm</fc> | ::: | |
| - | | 2 | RTBS | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489091-9110-000|FS91HE]] | <fc #0046ff>450 nm</fc> | <fc #009cff>455-479 nm</fc> | | + | | 2 | RTBS | 450 nm | <fc #009cff>455-479 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489091-9110-000|FS91HE]] | |
| - | | ::: | ::: | ::: | <fc #7bff00>538 nm</fc> | <fc #b3ff00>543-568 nm</fc> | | + | | ::: | ::: | 538 nm | <fc #b3ff00>543-568 nm</fc> | ::: | |
| - | | ::: | ::: | ::: | <fc #ff9b00>610 nm</fc> | <fc #ff0000>615-760 nm</fc> | | + | | ::: | ::: | 610 nm | <fc #ff0000>615-760 nm</fc> | ::: | |
| - | | 3 | RTBS | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489092-9110-000|FS92HE]] | <fc #a600ff>405 nm</fc> | <fc #5400ff>410-440 nm</fc> | | + | | 3 | RTBS | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489092-9110-000|FS92HE]] | |
| - | | ::: | ::: | ::: | <fc #00ffe0>493 nm</fc> | <fc #46ff00>499-549 nm</fc> | | + | | ::: | ::: | 493 nm | <fc #46ff00>499-549 nm</fc> | ::: | |
| - | | ::: | ::: | ::: | <fc #ff9b00>610 nm</fc> | <fc #ff0000>616-761 nm</fc> | | + | | ::: | ::: | 610 nm | <fc #ff0000>616-761 nm</fc> | ::: | |
| - | | 4 | RDBS | FS93HE | <fc #00ffe0>493 nm</fc> | <fc #1fff00>498-530 nm</fc> | | + | | 4 | RDBS | 493 nm | <fc #1fff00>498-530 nm</fc> | FS93HE | |
| - | | ::: | ::: | ::: | <fc #f0ff00>575 nm</fc> | <fc #ffad00>580-630 nm</fc> | | + | | ::: | ::: | 575 nm | <fc #ffad00>580-630 nm</fc> | ::: | |
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Model ^ Immersion ^ WD (mm) ^ NA ^ Magnification changer ^ Magnification ^ | ^ Model ^ Immersion ^ WD (mm) ^ NA ^ Magnification changer ^ Magnification ^ | ||
| - | | Plan-Apochromat 5x / 0.35 | Air | 5.10 | 0.12 | 0.5x | 2.5x | | + | | <fc #ed0707>Plan-Apochromat 5x / 0.35</fc> | Air | 5.10 | 0.12 | 0.5x | 2.5x | |
| | ::: | ::: | ::: | 0.25 | 1x | 5x | | | ::: | ::: | ::: | 0.25 | 1x | 5x | | ||
| | ::: | ::: | ::: | 0.35 | 2x | 10x | | | ::: | ::: | ::: | 0.35 | 2x | 10x | | ||
| - | | Plan-Apochromat 20x / 0.7 | Air | 2.20 | 0.35 | 0.5x | 10x | | + | | <fc #f9fb1a>Plan-Apochromat 20x / 0.7</fc> | Air | 2.20 | 0.35 | 0.5x | 10x | |
| | ::: | ::: | ::: | 0.70 | 1x | 20x | | | ::: | ::: | ::: | 0.70 | 1x | 20x | | ||
| | ::: | ::: | ::: | ::: | 2x | 40x | | | ::: | ::: | ::: | ::: | 2x | 40x | | ||
| - | | Plan-Apochromat 20x / 0.95 | Air | 0.76 | 0.50 | 0.5x | 10x | | + | | <fc #32cb35>Plan-Apochromat 20x / 0.95</fc> | Air | 0.76 | 0.50 | 0.5x | 10x | |
| | ::: | ::: | ::: | 0.80 | 1x | 20x | | | ::: | ::: | ::: | 0.80 | 1x | 20x | | ||
| | ::: | ::: | ::: | 0.95 | 2x | 40x | | | ::: | ::: | ::: | 0.95 | 2x | 40x | | ||
| - | | Plan-Apochromat 50x / 1.2 W | Water | 0.84 | 1.20 | 0.5x | 25x | | + | | <fc #2190fd>Plan-Apochromat 50x / 1.2 W</fc> | Water | 0.84 | 1.20 | 0.5x | 25x | |
| | ::: | ::: | ::: | ::: | 1x | 50x | | | ::: | ::: | ::: | ::: | 1x | 50x | | ||
| | ::: | ::: | ::: | ::: | 2x | 100x | | | ::: | ::: | ::: | ::: | 2x | 100x | | ||
zeiss_celldiscoverer_7.1765295985.txt.gz · Last modified: 2025/12/09 16:59 by bioimaging
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