zeiss_celldiscoverer_7
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zeiss_celldiscoverer_7 [2024/07/22 01:49] bioimaging |
zeiss_celldiscoverer_7 [2025/01/14 16:37] (current) bioimaging [Microscope overview] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_discoverer_7_name.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_zeiss_cd7_with_lsm_900_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cd7_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cd7_wiki_2024.png" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cd7_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cd7_wiki_2024.png" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cd7_map_2024.png">Room P0-A-30</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cd7_map_2024.png">[Lisbon] Room P0-A-30</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/imaging-systems/celldiscoverer-7.html">Celldiscoverer 7 with LSM 900</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/imaging-systems/celldiscoverer-7.html">Celldiscoverer 7 with LSM 900</a><br> | ||
| Line 40: | Line 40: | ||
| * [[https://www.zeiss.com/microscopy/en/resources/insights-hub/life-sciences/the-smart-microscope-for-automated-workflows-celldiscoverer-7-with-lsm-900.html|ZEISS Webinar: LSM 980 Airyscan 2 Multiplex mode]] | * [[https://www.zeiss.com/microscopy/en/resources/insights-hub/life-sciences/the-smart-microscope-for-automated-workflows-celldiscoverer-7-with-lsm-900.html|ZEISS Webinar: LSM 980 Airyscan 2 Multiplex mode]] | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| + | |||
| + | ===== Booking Rules ===== | ||
| + | - **Users** can book at maximum **8 hours per week** | ||
| + | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00** | ||
| + | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
| ===== System components ===== | ===== System components ===== | ||
| - | ==== LEDs ==== | + | ==== LASERs (confocal) ==== |
| + | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| + | | 405 | 405 nm | 5 mW | | ||
| + | | 488 | 488 nm | 10 mW | | ||
| + | | 561 | 561 nm | 10 mW | | ||
| + | | 640 | 640 nm | 5 mW | | ||
| + | |||
| + | ==== LEDs (widefield) ==== | ||
| ^ Line ^ Wavelength ^ | ^ Line ^ Wavelength ^ | ||
| | UV | 385 nm | | | UV | 385 nm | | ||
| Line 54: | Line 66: | ||
| | R | 625 nm | | | R | 625 nm | | ||
| - | ==== Filtersets ==== | + | ==== Filtersets (widefield) ==== |
| ^ Position ^ Filterset ^ Reference ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Dichroic ^ Emission ^ | ||
| Line 84: | Line 96: | ||
| | ::: | ::: | ::: | ::: | 1x | 50x | | | ::: | ::: | ::: | ::: | 1x | 50x | | ||
| | ::: | ::: | ::: | ::: | 2x | 100x | | | ::: | ::: | ::: | ::: | 2x | 100x | | ||
| - | ==== Cameras ==== | + | |
| + | ==== Camera (widefield) ==== | ||
| ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
| - | | Axiocam 702 mono | CMOS | 1920 x 1216 | 5.86 x 5.86 | | ||
| | [[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-712-mono.html|Axiocam 712 mono CMOS]] | CMOS | 4096 x 3008 | 3.45 x 3.45 | | | [[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-712-mono.html|Axiocam 712 mono CMOS]] | CMOS | 4096 x 3008 | 3.45 x 3.45 | | ||
| + | |||
| + | ===== How to start your session ===== | ||
| + | |||
| + | The system should be always on, you only need to: | ||
| + | * Activate the CD7 by pressing the "Standby" button (to the left of the touch panel) | ||
| + | {{::cd7_standby.png?250|}} | ||
| + | * Turn on the computer | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start **ZEN** | ||
| + | * Open the **Main valve** of the CO2 tank (if needed) - do not change the pressure settings! | ||
| + | {{::cd7_co2.png?130|}} | ||
| + | |||
| + | ===== How to end your session ===== | ||
| + | |||
| + | If there is another user for this microscope in the __next hour__: | ||
| + | * Exit the ZEN software, leave the lasers on (if used) | ||
| + | * Log off the computer | ||
| + | Else: | ||
| + | * Close the main valve of the CO2 tank (if used) | ||
| + | * Turn off the lasers on **ZEN** | ||
| + | * Exit the ZEN software | ||
| + | * Shutdown the computer | ||
| + | * Press the "Standby" button (to the left of the touch panel). | ||
| + | |||
| + | ===== Microscope Turn On Procedure (after complete shutdown) ===== | ||
| + | |||
| + | If the system is completely off, please follow this procedure: | ||
| + | |||
| + | * Turn on the ''MAIN SWITCH'' (bottom right of the CD7) | ||
| + | {{::cd7_main_switch.png?250|}} | ||
| + | * Activate the CD7 by pressing the "Standby" button (to the left of the touch panel) | ||
| + | * Turn on the computer | ||
| + | * Log into Windows with your Agendo credentials | ||
| + | * Start the **ZEN** software | ||
| + | * Open the **Main valve** of the CO2 tank (if needed) - do not change the pressure settings! | ||
| + | |||
| + | ===== Microscope Turn Off Procedure (for complete shutdown) ===== | ||
| + | |||
| + | Please only shutdown the system if instructed to do so by the Bioimaging Unit. | ||
| + | |||
| + | * Close the main valve of the CO2 tank (if used) | ||
| + | * Turn off the lasers on **ZEN** | ||
| + | * Exit the ZEN software | ||
| + | * Shutdown the computer | ||
| + | * Press the "Standby" button (to the left of the touch panel) | ||
| + | * Turn off the ''MAIN SWITCH'' (bottom right of the CD7) | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_celldiscoverer_7.1721605781.txt.gz · Last modified: 2024/07/22 01:49 by bioimaging
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