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zeiss_cell_observer_sd
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zeiss_cell_observer_sd [2025/07/29 16:54] bioimaging [Booking Rules] |
zeiss_cell_observer_sd [2026/02/14 13:43] (current) bioimaging [Filtersets (Widefield)] |
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| ===== System components ===== | ===== System components ===== | ||
| - | ==== Objectives ==== | + | ==== LASERs (confocal) ==== |
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | ^ LASER ^ Wavelength ^ Maximum Power ^ |
| - | | 10x | EC Plan-Neofluar Ph1 | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] | | + | | Violet | <fc #a600ff>405 nm</fc> | 50 mW | |
| - | | 20x | Plan-Apochromat Ph2 | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420651-9911-000/Objective-Plan-Apochromat-20x-0.8-Ph2-M27|420651-9911-000]] | | + | | Blue | <fc #00f7ff>488 nm</fc> | 100 mW | |
| - | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 75 mW | |
| - | | 40x | Plan-Apochromat DIC | Oil | 1.40 | 0.13 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420762-9900-000/Objective-Plan-Apochromat-40x-1.4-Oil-DIC-M27|420762-9900-000]] | | + | | Red | <fc #ff2b00>638 nm</fc> | 75 mW | |
| - | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | + | |
| + | ==== LEDs (widefield) ==== | ||
| - | **Upon request:** | + | ^ Line ^ Wavelength ^ |
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | | UV | <fc #be00cc>365 nm</fc> | |
| - | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | + | | B | <fc #00a9ff>470 nm</fc> | |
| - | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | + | | G | <fc #d2ff00>565 nm</fc> | |
| + | | R | <fc #ff6300>625 nm</fc> | | ||
| - | ==== LASERs (Confocal) ==== | + | ==== Filtersets (Widefield) ==== |
| - | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | + | |
| - | | Solid State Laser 405 | 405 nm | 50 mW | | + | |
| - | | Solid State Laser 488 | 488 nm | 100 mW | | + | |
| - | | Solid State Laser 561 | 561 nm | 75 mW | | + | |
| - | | Solid State Laser 638 | 638 nm | 75 mW | | + | |
| - | ==== Emission Filters (Confocal) ==== | + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ |
| + | | 1 | Green | 450-490 nm | 495 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489038-9901-400|FS38HE]] | | ||
| + | | 2 | Red | 538-562 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489043-9901-400|FS43HE]] | | ||
| + | | 3 | Blue | 335-383 nm | 395 nm | <fc #0028ff>420-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488049-9901-400|FS49]] | | ||
| + | | 4 | Far Red | 625-655 nm | 660 nm | <fc #ff0000>665-715 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488050-9901-400|FS50]] | | ||
| + | | 5 | QUAD | 357-393 nm | 400 nm | <fc #7e00db>407-412 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489081-9010-000| FS81HE]] | | ||
| + | | ::: | ::: | 473-496 nm | 503 nm | <fc #36ff00>511-528 nm</fc> | ::: | | ||
| + | | ::: | ::: | 545-562 nm | 570 nm | <fc #ffdf00>577-602 nm</fc> | ::: | | ||
| + | | ::: | ::: | 620-642 nm | 650 nm | <fc #ff0000>658-704 nm</fc> | ::: | | ||
| + | | 6 | Empty ||||| | ||
| - | ^ Position ^ Transmission ^ | + | ==== Objectives ==== |
| - | | 1: BP 450/50 | 425-475 nm | | + | |
| - | | 2: TBP 526 601 688 | 510-542 nm | | + | |
| - | | ::: | 586-616 nm | | + | |
| - | | ::: | 663-713 nm | | + | |
| - | | 3: none | | | + | |
| - | | 4: FE01-520/35 | 502-537 nm | | + | |
| - | | 5: BP 600 50 | 575-625 nm | | + | |
| - | | 6: BP 690/50 | 665-715 nm | | + | |
| - | ==== LEDs (Widefield) ==== | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ |
| - | ^ Line ^ Wavelength ^ | + | | <fc #fef042>10x</fc> | EC Plan-Neofluar Ph1 | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] | |
| - | | UV | 365 nm | | + | | <fc #0aa05c>20x</fc> | Plan-Apochromat Ph2 | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420651-9911-000/Objective-Plan-Apochromat-20x-0.8-Ph2-M27|420651-9911-000]] | |
| - | | B | 470 nm | | + | | <fc #01b3f5>40x</fc> | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | |
| - | | G | 565 nm | | + | | <fc #01b3f5>40x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.13 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420762-9900-000/Objective-Plan-Apochromat-40x-1.4-Oil-DIC-M27|420762-9900-000]] | |
| - | | R | 625 nm | | + | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | |
| - | ==== Filtersets (Widefield) ==== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | **Upon request:** |
| - | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ |
| - | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570 nm | 570-640 nm | | + | | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | |
| - | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 395 nm | 420-470 nm | | + | | <fc #0aa05c>25x</fc> | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| - | | 4 | Far Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 660 nm | 665-715 nm | | + | |
| - | | 5 | QUAD | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489081-9010-000&o=| FS81HE]] | 357-393 nm | 400 nm | 407-412 nm | | + | |
| - | | ::: | ::: | ::: | 473-496 nm | 503 nm | 511-528 nm | | + | |
| - | | ::: | ::: | ::: | 545-562 nm | 570 nm | 577-602 nm | | + | |
| - | | ::: | ::: | ::: | 620-642 nm | 650 nm | 658-704 nm | | + | |
| - | | 6 | Empty ||||| | + | |
| + | ==== Emission Filters CSU X1 ==== | ||
| + | |||
| + | ^ Position ^ Setting ^ Transmission ^ | ||
| + | | 1 | 450 | <fc #0046ff>425-475 nm</fc> | | ||
| + | | 2 | TBP 526 601 688 | <fc #4eff00>510-542 nm</fc> | | ||
| + | | ::: | ::: | <fc #ffbb00>586-616 nm</fc> | | ||
| + | | ::: | ::: | <fc #ff0000>663-713 nm</fc> | | ||
| + | | 3 | none | | | ||
| + | | 4 | 520 | <fc #36ff00>502-537 nm</fc> | | ||
| + | | 5 | 600 | <fc #ffbe00>575-625 nm</fc> | | ||
| + | | 6 | 690 | <fc #ff0000>665-715 nm</fc> | | ||
| ==== Cameras ==== | ==== Cameras ==== | ||
| + | |||
| ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ | ||
| | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | | ||
| Line 126: | Line 128: | ||
| Else: | Else: | ||
| * Exit the **ZEN** software | * Exit the **ZEN** software | ||
| - | * Log off the computer | + | * Turn off the computer |
| * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) | ||
| * Turn off the black socket outlets by reverse order (3→2→1) | * Turn off the black socket outlets by reverse order (3→2→1) | ||
zeiss_cell_observer_sd.1753800872.txt.gz · Last modified: 2025/07/29 16:54 by bioimaging
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