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zeiss_cell_observer_sd [2024/07/24 00:16] bioimaging |
zeiss_cell_observer_sd [2026/02/14 13:43] (current) bioimaging [Filtersets (Widefield)] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_zeiss_cell_observer_sd_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2024.png" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2024.png" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_map_new.png">[Lisbon] Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: Cell Observer SD<br> | <b>Model</b>: Cell Observer SD<br> | ||
| Line 42: | Line 42: | ||
| * | [[https://www.youtube.com/watch?v=DqqV6cs3mZg|Multi-Position Time Series with Definite Focus.2]] | * | [[https://www.youtube.com/watch?v=DqqV6cs3mZg|Multi-Position Time Series with Definite Focus.2]] | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| - | ===== Booking Rules ===== | ||
| - | - **Users** can book at maximum **8 hours per week** | ||
| - | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00** | ||
| - | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
| ===== System components ===== | ===== System components ===== | ||
| - | ==== Objectives ==== | + | ==== LASERs (confocal) ==== |
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | ^ LASER ^ Wavelength ^ Maximum Power ^ |
| - | | 10x | EC Plan-Neofluar Ph1 | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] | | + | | Violet | <fc #a600ff>405 nm</fc> | 50 mW | |
| - | | 20x | Plan-Apochromat Ph2 | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420651-9911-000/Objective-Plan-Apochromat-20x-0.8-Ph2-M27|420651-9911-000]] | | + | | Blue | <fc #00f7ff>488 nm</fc> | 100 mW | |
| - | | 40x | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 75 mW | |
| - | | 40x | Plan-Apochromat DIC | Oil | 1.40 | 0.13 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420762-9900-000/Objective-Plan-Apochromat-40x-1.4-Oil-DIC-M27|420762-9900-000]] | | + | | Red | <fc #ff2b00>638 nm</fc> | 75 mW | |
| - | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | + | |
| - | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | + | |
| - | **Upon request:** | + | ==== LEDs (widefield) ==== |
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | |
| - | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | + | |
| - | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | + | |
| - | + | ||
| - | ==== LASERs (Confocal) ==== | + | |
| - | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | + | |
| - | | Solid State Laser 405 | 405 nm | 50 mW | | + | |
| - | | Solid State Laser 488 | 488 nm | 100 mW | | + | |
| - | | Solid State Laser 561 | 561 nm | 75 mW | | + | |
| - | | Solid State Laser 638 | 638 nm | 75 mW | | + | |
| - | ==== Emission Filters (Confocal) ==== | ||
| - | |||
| - | ^ Position ^ Transmission ^ | ||
| - | | 1: BP 450/50 | 425-475 nm | | ||
| - | | 2: TBP 526 601 688 | 510-542 nm | | ||
| - | | ::: | 586-616 nm | | ||
| - | | ::: | 663-713 nm | | ||
| - | | 3: none | | | ||
| - | | 4: FE01-520/35 | 502-537 nm | | ||
| - | | 5: BP 600 50 | 575-625 nm | | ||
| - | | 6: BP 690/50 | 665-715 nm | | ||
| - | |||
| - | ==== LEDs (Widefield) ==== | ||
| ^ Line ^ Wavelength ^ | ^ Line ^ Wavelength ^ | ||
| - | | UV | 365 nm | | + | | UV | <fc #be00cc>365 nm</fc> | |
| - | | B | 470 nm | | + | | B | <fc #00a9ff>470 nm</fc> | |
| - | | G | 565 nm | | + | | G | <fc #d2ff00>565 nm</fc> | |
| - | | R | 625 nm | | + | | R | <fc #ff6300>625 nm</fc> | |
| ==== Filtersets (Widefield) ==== | ==== Filtersets (Widefield) ==== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ |
| - | | 1 | Green | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | 450-490 nm | 495 nm | 500-550 nm | | + | | 1 | Green | 450-490 nm | 495 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489038-9901-400|FS38HE]] | |
| - | | 2 | Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | 538-562 nm | 570 nm | 570-640 nm | | + | | 2 | Red | 538-562 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489043-9901-400|FS43HE]] | |
| - | | 3 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | 335-383 nm | 395 nm | 420-470 nm | | + | | 3 | Blue | 335-383 nm | 395 nm | <fc #0028ff>420-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488049-9901-400|FS49]] | |
| - | | 4 | Far Red | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | 625-655 nm | 660 nm | 665-715 nm | | + | | 4 | Far Red | 625-655 nm | 660 nm | <fc #ff0000>665-715 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488050-9901-400|FS50]] | |
| - | | 5 | QUAD | [[https://www.micro-shop.zeiss.com/?s=4237481102cf7b&l=en&p=us&f=f&a=v&b=f&id=489081-9010-000&o=| FS81HE]] | 357-393 nm | 400 nm | 407-412 nm | | + | | 5 | QUAD | 357-393 nm | 400 nm | <fc #7e00db>407-412 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489081-9010-000| FS81HE]] | |
| - | | ::: | ::: | ::: | 473-496 nm | 503 nm | 511-528 nm | | + | | ::: | ::: | 473-496 nm | 503 nm | <fc #36ff00>511-528 nm</fc> | ::: | |
| - | | ::: | ::: | ::: | 545-562 nm | 570 nm | 577-602 nm | | + | | ::: | ::: | 545-562 nm | 570 nm | <fc #ffdf00>577-602 nm</fc> | ::: | |
| - | | ::: | ::: | ::: | 620-642 nm | 650 nm | 658-704 nm | | + | | ::: | ::: | 620-642 nm | 650 nm | <fc #ff0000>658-704 nm</fc> | ::: | |
| | 6 | Empty ||||| | | 6 | Empty ||||| | ||
| + | ==== Objectives ==== | ||
| + | |||
| + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| + | | <fc #fef042>10x</fc> | EC Plan-Neofluar Ph1 | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420341-9911-000/Objective-EC-Plan-Neofluar-10x-0.30-Ph1-M27|420341-9911-000]] | | ||
| + | | <fc #0aa05c>20x</fc> | Plan-Apochromat Ph2 | Air | 0.80 | 0.55 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420651-9911-000/Objective-Plan-Apochromat-20x-0.8-Ph2-M27|420651-9911-000]] | | ||
| + | | <fc #01b3f5>40x</fc> | LD C-Apochromat Corr | Water | 1.10 | 0.62 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421867-9970-000/Objective-LD-C-Apochromat-40x-1.1-W-Corr-M27|421867-9970-000]] | | ||
| + | | <fc #01b3f5>40x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.13 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420762-9900-000/Objective-Plan-Apochromat-40x-1.4-Oil-DIC-M27|420762-9900-000]] | | ||
| + | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | ||
| + | |||
| + | |||
| + | **Upon request:** | ||
| + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| + | | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
| + | | <fc #0aa05c>25x</fc> | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9972-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | ||
| + | |||
| + | ==== Emission Filters CSU X1 ==== | ||
| + | |||
| + | ^ Position ^ Setting ^ Transmission ^ | ||
| + | | 1 | 450 | <fc #0046ff>425-475 nm</fc> | | ||
| + | | 2 | TBP 526 601 688 | <fc #4eff00>510-542 nm</fc> | | ||
| + | | ::: | ::: | <fc #ffbb00>586-616 nm</fc> | | ||
| + | | ::: | ::: | <fc #ff0000>663-713 nm</fc> | | ||
| + | | 3 | none | | | ||
| + | | 4 | 520 | <fc #36ff00>502-537 nm</fc> | | ||
| + | | 5 | 600 | <fc #ffbe00>575-625 nm</fc> | | ||
| + | | 6 | 690 | <fc #ff0000>665-715 nm</fc> | | ||
| ==== Cameras ==== | ==== Cameras ==== | ||
| + | |||
| ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Mode ^ | ||
| | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | | | [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Photometrics Evolve 512]] | EMCCD | 512 x 512 | 16 x 16 | > 90% | Confocal | | ||
| Line 113: | Line 112: | ||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the HXP 200 fluorescent light source (if used) | * Turn on the HXP 200 fluorescent light source (if used) | ||
| - | * Turn on the computer (underneath the microscope table) | + | * Turn on the computer (if needed) |
| * Login in to Windows with your Agendo credentials | * Login in to Windows with your Agendo credentials | ||
| * Turn on the black socket outlet **number 1** (back left corner of microscope table) | * Turn on the black socket outlet **number 1** (back left corner of microscope table) | ||
| {{::sd_power.png?200|}} | {{::sd_power.png?200|}} | ||
| - | * Wait for the Definite Focus controller to show the App-Ver. and OS-Image info | + | * Wait for the Definite Focus controller to show App-Ver. + OS-Image info |
| {{::df_on.png?500|}} | {{::df_on.png?500|}} | ||
| * Turn on the black socket outlet **number 2** | * Turn on the black socket outlet **number 2** | ||
| Line 126: | Line 125: | ||
| * Exit the **ZEN** software | * Exit the **ZEN** software | ||
| * Log off the computer | * Log off the computer | ||
| - | * Clean up immersion objectives | + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) |
| Else: | Else: | ||
| * Exit the **ZEN** software | * Exit the **ZEN** software | ||
| - | * Shut down the computer (not the UPS!) | + | * Turn off the computer |
| - | * Clean up immersion objectives | + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) |
| * Turn off the black socket outlets by reverse order (3→2→1) | * Turn off the black socket outlets by reverse order (3→2→1) | ||
| * Turn off the HXP 200 fluorescent light source | * Turn off the HXP 200 fluorescent light source | ||
zeiss_cell_observer_sd.1721773006.txt.gz · Last modified: 2024/07/24 00:16 by bioimaging
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