zeiss_cell_observer_sd
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zeiss_cell_observer_sd [2024/01/28 11:44] bioimaging [Cameras] |
zeiss_cell_observer_sd [2025/07/29 16:54] (current) bioimaging [Booking Rules] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_zeiss_cell_observer_sd_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2022.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_wiki_2024.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_map_new.png">Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_cell_observer_sd_map_new.png">[Lisbon] Room P2-A-22</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47217) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: Cell Observer SD<br> | <b>Model</b>: Cell Observer SD<br> | ||
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| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
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|   <br> |   <br> | ||
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| {{ :yokogawa_sd.png?350|}} The Zeiss Cell Observer SD //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a stage top incubator for CO2 supply and with Definite Focus.2 for hardware focus control during large tile or multi-position/time-lapse acquisitions (check tutorial videos below). You can also use the system as a widefield microscope, using fast-swicthing LEDs for illumination (Zeiss Colibri.2) and a sCMOS camera for acquisition. | {{ :yokogawa_sd.png?350|}} The Zeiss Cell Observer SD //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed. It is an inverted microscope ideal for live cell applications which require fast acquisition speeds rather than high resolution images. The scanning unit achieves confocality by directing light through a spinning disk with many small pinholes. Images are then acquired with a sensitive EMCCD which allows for very small exposure times but is limited in resolution to 512x512 pixels. The stage is motorized and furthermore equipped with a piezo for Z displacement so fast 4D imaging is possible in multiple stage positions. The system is also equipped with a large size incubator for temperature control, a stage top incubator for CO2 supply and with Definite Focus.2 for hardware focus control during large tile or multi-position/time-lapse acquisitions (check tutorial videos below). You can also use the system as a widefield microscope, using fast-swicthing LEDs for illumination (Zeiss Colibri.2) and a sCMOS camera for acquisition. | ||
| - | * **Microscope**: [[http://www.zeiss.com/microscopy/en_de/products/light-microscopes/axio-observer-for-biology.html|Zeiss Axio Observer]] | + | * **Microscope**: [[https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-observer-for-life-science-research.html|Zeiss Axio Observer]] |
| + | * **LED Illumination**: [[https://www.zeiss.com/microscopy/en/products/accessories/colibri.html|Zeiss Colibri 5]] | ||
| * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit//|Yokogawa CSU-X1]] | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-x1-confocal-scanner-unit//|Yokogawa CSU-X1]] | ||
| * **EMCCD Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | * **EMCCD Camera**: [[https://www.photometrics.com/wp-content/uploads/2019/10/Evolve512-Datasheet.pdf|Evolve 512 EMCCD]] | ||
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| * | [[https://www.youtube.com/watch?v=DqqV6cs3mZg|Multi-Position Time Series with Definite Focus.2]] | * | [[https://www.youtube.com/watch?v=DqqV6cs3mZg|Multi-Position Time Series with Definite Focus.2]] | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| - | ===== Booking Rules ===== | ||
| - | - **Users** can book at maximum **8 hours per week** | ||
| - | - This usage restriction does not apply for **weekends** and for working days **before 9:00** and **after 21:00** | ||
| - | - Exceptions to these rules require **approval from [[joserino@medicina.ulisboa.pt|José Rino]]**. | ||
| ===== System components ===== | ===== System components ===== | ||
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| | 40x | Plan-Apochromat DIC | Oil | 1.40 | 0.13 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420762-9900-000/Objective-Plan-Apochromat-40x-1.4-Oil-DIC-M27|420762-9900-000]] | | | 40x | Plan-Apochromat DIC | Oil | 1.40 | 0.13 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420762-9900-000/Objective-Plan-Apochromat-40x-1.4-Oil-DIC-M27|420762-9900-000]] | | ||
| | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | ||
| - | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | + | |
| **Upon request:** | **Upon request:** | ||
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| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the HXP 200 fluorescent light source (if used) | * Turn on the HXP 200 fluorescent light source (if used) | ||
| - | * Turn on the computer (underneath the microscope table) | + | * Turn on the computer (if needed) |
| * Login in to Windows with your Agendo credentials | * Login in to Windows with your Agendo credentials | ||
| * Turn on the black socket outlet **number 1** (back left corner of microscope table) | * Turn on the black socket outlet **number 1** (back left corner of microscope table) | ||
| {{::sd_power.png?200|}} | {{::sd_power.png?200|}} | ||
| - | * Wait for the Definite Focus controller to show the App-Ver. and OS-Image info | + | * Wait for the Definite Focus controller to show App-Ver. + OS-Image info |
| {{::df_on.png?500|}} | {{::df_on.png?500|}} | ||
| * Turn on the black socket outlet **number 2** | * Turn on the black socket outlet **number 2** | ||
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| * Exit the **ZEN** software | * Exit the **ZEN** software | ||
| * Log off the computer | * Log off the computer | ||
| - | * Clean up immersion objectives | + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) |
| Else: | Else: | ||
| * Exit the **ZEN** software | * Exit the **ZEN** software | ||
| - | * Shut down the computer (not the UPS!) | + | * Log off the computer |
| - | * Clean up immersion objectives | + | * Clean up immersion objectives (gently wipe with optic cleaning paper and EtOH embedded paper) |
| * Turn off the black socket outlets by reverse order (3→2→1) | * Turn off the black socket outlets by reverse order (3→2→1) | ||
| * Turn off the HXP 200 fluorescent light source | * Turn off the HXP 200 fluorescent light source | ||
zeiss_cell_observer_sd.1706438675.txt.gz · Last modified: 2024/01/28 11:44 by bioimaging
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