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zeiss_cell_observer [2025/12/12 12:08] bioimaging [Objectives] |
zeiss_cell_observer [2026/02/13 22:56] (current) bioimaging [Filtersets] |
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| <b>Model</b>: Axio Observer<br> | <b>Model</b>: Axio Observer<br> | ||
| <b>Nickname</b>: "Cell Observer"<br> | <b>Nickname</b>: "Cell Observer"<br> | ||
| - | <b>Software</b>: ZEN 3.0 (blue edition)<br> | + | <b>Software</b>: ZEN 3.2 (blue edition)<br> |
| <b>Year</b>: 2015 <br> | <b>Year</b>: 2015 <br> | ||
| <b>SN</b>: 3851002396<br> | <b>SN</b>: 3851002396<br> | ||
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| ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| - | | 1 | Green | 450-490 nm | 495 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | | + | | 1 | Green | 450-490 nm | 495 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489038-9901-400|FS38HE]] | |
| - | | 2 | Orange | 538-562 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=489043-9901-000&o=|FS43HE]] | | + | | 2 | Orange | 538-562 nm | 570 nm | <fc #ffad00>570-640 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489043-9901-400|FS43HE]] | |
| - | | 3 | Red | 625-655 nm | 660 nm | <fc #ff0000>665-715 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=488050-9901-000&o=|FS50]] | | + | | 3 | Red | 625-655 nm | 660 nm | <fc #ff0000>665-715 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488050-9901-000|FS50]] | |
| | 4 | Analyzer DIC ||||| | | 4 | Analyzer DIC ||||| | ||
| - | | 5 | QUAD | 370-400 nm | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000| FS90HE]] | | + | | 5 | QUAD | 370-400 nm | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489090-9100-400| FS90HE]] | |
| | ::: | ::: | 450-488 nm | 493 nm | <fc #1fff00>499-529 nm</fc> | ::: | | | ::: | ::: | 450-488 nm | 493 nm | <fc #1fff00>499-529 nm</fc> | ::: | | ||
| | ::: | ::: | 540-570 nm | 575 nm | <fc #ffd900>580-605 nm</fc> | ::: | | | ::: | ::: | 540-570 nm | 575 nm | <fc #ffd900>580-605 nm</fc> | ::: | | ||
| | ::: | ::: | 615-648 nm | 653 nm | <fc #ef0000>659-759 nm</fc> | ::: | | | ::: | ::: | 615-648 nm | 653 nm | <fc #ef0000>659-759 nm</fc> | ::: | | ||
| - | | 6 | DAPI | 335-383 nm | 395 nm | <fc #0028ff>420-470 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=23108437692794b&l=en&p=us&f=f&a=v&b=f&id=488049-9901-000&o=|FS49]] | | + | | 6 | DAPI | 335-383 nm | 395 nm | <fc #0028ff>420-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488049-9901-400|FS49]] | |
| ==== Objectives ==== | ==== Objectives ==== | ||
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| | <fc #df2b37>5x</fc> | EC Plan-NeoFluar | Air | 0.16 | 18.50 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | | | <fc #df2b37>5x</fc> | EC Plan-NeoFluar | Air | 0.16 | 18.50 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | | ||
| | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9901-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9901-000]] | | ||
| - | | 25x | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | | + | | <fc #0aa05c>25x</fc> | LCI Plan-Neofluar Corr DIC | Oil/Glyc/W | 0.80 | 0.21 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420852-9973-000/Objective-LCI-Plan-Neofluar-25x-0.8-Imm-Corr-DIC-M27|420852-9972-000]] | |
| | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | | <fc #01b3f5>40x</fc> | Plan-Apochromat Corr | Air | 0.95 | 0.25 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420660-9970-000/Objective-Plan-Apochromat-40x-0.95-Corr-M27|420660-9970-000]] | | ||
| | <fc #01b3f5>40x</fc> | Plan-Neofluar Ph3 | Oil | 1.30 | 0.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440451-9903-000/Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] | | | <fc #01b3f5>40x</fc> | Plan-Neofluar Ph3 | Oil | 1.30 | 0.20 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440451-9903-000/Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] | | ||
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| ===== System Turn Off procedures ===== | ===== System Turn Off procedures ===== | ||
| If there is another user for this microscope is the __next hour__: | If there is another user for this microscope is the __next hour__: | ||
| + | * Exit the ZEN software | ||
| + | * Log off the computer | ||
| * Leave the fluorescent light source on | * Leave the fluorescent light source on | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| Else: | Else: | ||
| * Exit the ZEN software | * Exit the ZEN software | ||
| - | * Log off the computer | + | * Turn off the computer |
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| * Turn off the microscope power supply (**2** on remote control) | * Turn off the microscope power supply (**2** on remote control) | ||
zeiss_cell_observer.1765537722.txt.gz · Last modified: 2025/12/12 12:08 by bioimaging
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