zeiss_axiovert_200m
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zeiss_axiovert_200m [2022/09/01 15:03] bioimaging |
zeiss_axiovert_200m [2025/01/14 16:34] (current) bioimaging [Microscope overview] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_zeiss_axiovert_200m_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_2022.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_2024.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_map_new_p2a24.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_map_new_p2a24.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: Axiovert 200M<br> | <b>Model</b>: Axiovert 200M<br> | ||
| <b>Nickname</b>: "Axiovert"<br> | <b>Nickname</b>: "Axiovert"<br> | ||
| - | <b>Software</b>: Metamorph 7.7.9.0<br> | + | <b>Software</b>: Metamorph 7.8.0.0<br> |
| <b>Year</b>: 2003 <br> | <b>Year</b>: 2003 <br> | ||
| <b>SN</b>: ZCA522000494<br> | <b>SN</b>: ZCA522000494<br> | ||
|   <br> |   <br> | ||
| - |   <br> | + | Data will be deleted after: <b>1 month</b> |
|   <br> |   <br> | ||
|   <br> |   <br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :widefield_lightpath.png?0x300}}The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics CoolSNAP HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus or the [[nikon_eclipse_ti|Nikon Eclipse Ti]]. If don't want to use deconvolution but need optical sectioning, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | + | {{ :widefield_lightpath.png?0x300}}The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera ([[https://labs.pbrc.edu/cellbiology/documents/CoolSNAPHQ.pdf|Photometrics CoolSNAP HQ CCD]]) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. It is also equipped with a color camera ([[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]]) for acquisition of histological stains with tile and stitching imaging. |
| * **Microscope**: Zeiss Axiovert 200 | * **Microscope**: Zeiss Axiovert 200 | ||
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| * **Color Camera**: [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | * **Color Camera**: [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== System components ===== | ===== System components ===== | ||
zeiss_axiovert_200m.1662037415.txt.gz ยท Last modified: 2022/09/01 15:03 by bioimaging
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