zeiss_axiovert_200m
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zeiss_axiovert_200m [2022/02/14 22:16] bioimaging [Microscope overview] |
zeiss_axiovert_200m [2025/12/12 11:57] (current) bioimaging |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_zeiss_axiovert_200m_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_new.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_new.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_wiki_2024.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_map_new_p2a24.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_axiovert_200m_map_new_p2a24.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| - | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">Carl Zeiss MicroImaging</a><br> | + | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> |
| <b>Model</b>: Axiovert 200M<br> | <b>Model</b>: Axiovert 200M<br> | ||
| <b>Nickname</b>: "Axiovert"<br> | <b>Nickname</b>: "Axiovert"<br> | ||
| - | <b>Software</b>: Metamorph 7.7.9.0<br> | + | <b>Software</b>: Metamorph 7.8.0.0<br> |
| <b>Year</b>: 2003 <br> | <b>Year</b>: 2003 <br> | ||
| <b>SN</b>: ZCA522000494<br> | <b>SN</b>: ZCA522000494<br> | ||
|   <br> |   <br> | ||
| + | Data will be deleted after: <b>1 month</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://my.agendo.science/calendar/?id=MjhjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Zeiss Axiovert 200M Booking</a> | ||
| - | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=zeiss_axiovert200m_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Axiovert 200M Quality Control</a> | → <a href="/facility/bioimaging/doku.php?id=zeiss_axiovert200m_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> Zeiss Axiovert 200M Quality Control</a> | ||
| <br> | <br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :widefield_lightpath.png?0x300}}The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera (Photometrics CoolSNAP HQ CCD) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. You can also perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If you are performing long time-lapse experiments in live samples (aqueous media) and need hardware focus control, check the [[zeiss_cell_observer|Zeiss Cell Observer]] with Definite Focus or the [[nikon_eclipse_ti|Nikon Eclipse Ti]]. If don't want to use deconvolution but need optical sectioning, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or the spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. If you have FISH or other fixed fluorescence slides try the [[leica_dm5000b|Leica DM5000B]] instead. | + | The Zeiss Axiovert 200M is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. Using the Metamorph software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. Its sensitive cooled CCD camera ([[https://labs.pbrc.edu/cellbiology/documents/CoolSNAPHQ.pdf|Photometrics CoolSNAP HQ CCD]]) allows you to capture weak fluorescent signals and minimize photobleaching/photodamage in light sensitive samples. It is also equipped with a color camera ([[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]]) for acquisition of histological stains with tile and stitching imaging. |
| * **Microscope**: Zeiss Axiovert 200 | * **Microscope**: Zeiss Axiovert 200 | ||
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| * **Color Camera**: [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | * **Color Camera**: [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | ||
| - | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== System components ===== | ===== System components ===== | ||
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| ==== Filtersets ==== | ==== Filtersets ==== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ |
| - | | 1 | Blue | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488001-9901-000&o=|FS01]] | 359-371 nm | 395 nm | > 397 nm | | + | | 1 | Blue | 359-371 nm | 395 nm | <fc #be00cc>> 397 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488001-9901-000&o=|FS01]] | |
| - | | 2 | Green BP | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488010-9901-000&o=|FS10]] | 450-490 nm | 510 nm | 515-565 nm | | + | | 2 | Green BP | 450-490 nm | 510 nm | <fc #81ff00>515-565 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488010-9901-000&o=|FS10]] | |
| - | | 3 | Red | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488020-9901-000&o=|FS20]] | 540-552 nm | 560 nm | 575-640 nm | | + | | 3 | Red | 540-552 nm | 560 nm | <fc #ffa600>575-640 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488020-9901-000&o=|FS20]] | |
| - | | 4 | Far Red | [[http://www.semrock.com/SetDetails.aspx?id=2983|Cy5-4040C-000]] | 608-648 nm | 660 nm | 672-712 nm | | + | | 4 | Far Red | 608-648 nm | 660 nm | <fc #ff0000>672-712 nm</fc> | [[http://www.semrock.com/SetDetails.aspx?id=2983|Cy5-4040C-000]] | |
| - | | 5 | A | none |||| | + | | 5 | none ||||| |
| **Upon request:** | **Upon request:** | ||
| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | 2 | Green LP | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488009-9901-000&o=|FS09]] | 450-490 nm | 510 nm | > 515 nm | | + | | 2 | Green LP | 450-490 nm | 510 nm | <fc #1fff00>> 515 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=163176628fcf844&l=en&p=us&f=f&a=v&b=f&id=488009-9901-000&o=|FS09]] | |
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ (color) 10 μm = ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ (color) 10 μm = ^ Reference ^ | ||
| - | | 10x | EC Plan-Neofluar | Air | 0.30 | 5.20 | 1.00 | 10 pixels | 23.3 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9900-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9900-000]] | | + | | <fc #fef042>10x</fc> | EC Plan-Neofluar | Air | 0.30 | 5.20 | 1.00 | 10 pixels | 23.3 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420340-9900-000/Objective-EC-Plan-Neofluar-10x-0.30-M27|420340-9900-000]] | |
| - | | 20x | EC Plan-Neofluar | Air | 0.50 | 2.00 | 0.48 | 21 pixels | 46.7 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440340-9904-000/Objective-EC-Plan-Neofluar-20x-0.5|440340-9904-000]] | | + | | <fc #0aa05c>20x</fc> | EC Plan-Neofluar | Air | 0.50 | 2.00 | 0.48 | 21 pixels | 46.7 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440340-9904-000/Objective-EC-Plan-Neofluar-20x-0.5|440340-9904-000]] | |
| - | | 40x | EC Plan-NeoFluar | Air | 0.75 | 0.71 | 0.24 | 41 pixels | 93.7 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440350-9903-000/Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]] | | + | | <fc #01b3f5>40x</fc> | EC Plan-NeoFluar | Air | 0.75 | 0.71 | 0.24 | 41 pixels | 93.7 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440350-9903-000/Objective-EC-Plan-Neofluar-40x-0.75|440350-9903-000]] | |
| - | | 63x | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | 0.15 | 65 pixels | - | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | | + | | <fc #2c80c0>63x</fc> | Plan-Apochromat DIC | Oil | 1.40 | 0.19 | 0.15 | 65 pixels | - | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420782-9900-000/Objective-Plan-Apochromat-63x-1.4-Oil-DIC-M27|420782-9900-000]] | |
| | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | 0.09 | 105 pixels | - | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | | 100x | α Plan-Apochromat DIC | Oil | 1.46 | 0.11 | 0.09 | 105 pixels | - | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420792-9800-000/Objective-alpha-Plan-Apochromat-100x-1.46-Oil-DIC-M27|420792-9800-000]] | | ||
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| **Upon request:** | **Upon request:** | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ pixel size* (μm) ^ 10 μm = ^ Reference ^ | ||
| - | | 1.25x | EC Epiplan-Neofluar | Air | 0.035 | 4 | 7.58 | 1.3 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/422310-9900-000/Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]] | | + | | <fc #88888a>1.25x</fc> | EC Epiplan-Neofluar | Air | 0.035 | 4 | 7.58 | 1.3 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/422310-9900-000/Objective-EC-Epiplan-Neofluar-1.25x-0.03-M27|422310-9900-000]] | |
| - | | 5x | EC Plan-NeoFluar | Air | 0.16 | 18.5 | 2.00 | 5 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | | + | | <fc #df2b37>5x</fc> | EC Plan-NeoFluar | Air | 0.16 | 18.5 | 2.00 | 5 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420330-9901-000/Objective-EC-Plan-Neofluar-5x-0.16-M27|420330-9901-000]] | |
| - | | 40x | Plan-Neofluar Ph3 | Oil | 1.30 | 0.20 | 0.24 | 41 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440451-9903-000/Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] | | + | | <fc #01b3f5>40x</fc> | Plan-Neofluar Ph3 | Oil | 1.30 | 0.20 | 0.24 | 41 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/440451-9903-000/Objective-EC-Plan-Neofluar-40x-1.30-Oil-Ph3|440451-9903-000]] | |
| - | | 63x | Plan-Apochromat Ph3 | Oil | 1.4 | 0.19 | 0.15 | 65 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420781-9910-000/Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] | | + | | <fc #2c80c0>63x</fc> | Plan-Apochromat Ph3 | Oil | 1.4 | 0.19 | 0.15 | 65 pixels | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/420781-9910-000/Objective-Plan-Apochromat-63-1.4-Oil-Ph3-M27|420781-9910-000]] | |
| *** pixel sizes for binning = 1** | *** pixel sizes for binning = 1** | ||
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| Y = -8995 μm | Y = -8995 μm | ||
| - | ===== System Turn On Procedures ===== | + | ===== Turn ON Procedure ===== |
| * Turn on the fluorescent light power source (if used) | * Turn on the fluorescent light power source (if used) | ||
| {{halide_710.jpg?80|}} | {{halide_710.jpg?80|}} | ||
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| * Turn on the CCD camera | * Turn on the CCD camera | ||
| {{ax_cam_on.jpg?100|}} | {{ax_cam_on.jpg?100|}} | ||
| - | * Turn on the computer | + | * Turn on the computer (if needed) |
| * Log in to Windows (Bioimaging User) | * Log in to Windows (Bioimaging User) | ||
| * Start the Metamorph software | * Start the Metamorph software | ||
| * If the software doesn't detect the camera, power cycle the camera and try again | * If the software doesn't detect the camera, power cycle the camera and try again | ||
| - | ===== Microscope Turn Off procedures ===== | + | * |
| + | ===== Turn OFF procedure ===== | ||
| If there is another user for this microscope in the __next hour__: | If there is another user for this microscope in the __next hour__: | ||
| Line 101: | Line 101: | ||
| Else: | Else: | ||
| * Clean up immersion objectives | * Clean up immersion objectives | ||
| - | * Shutdown the computer | + | * Log off the computer |
| * Turn off the camera | * Turn off the camera | ||
| * Turn off the Prior stage controller | * Turn off the Prior stage controller | ||
zeiss_axiovert_200m.1644873407.txt.gz · Last modified: 2022/02/14 22:16 by bioimaging
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