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zeiss_apotome [2025/12/12 11:36]
bioimaging
zeiss_apotome [2026/04/17 23:46] (current)
bioimaging
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 <​b>​Model</​b>:​ <a href="​https://​www.zeiss.com/​microscopy/​en/​products/​light-microscopes/​widefield-microscopes/​axio-imager-2-for-life-science-research.html">​Zeiss Axio Imager 2 with Apotome 2</​a><​br>​ <​b>​Model</​b>:​ <a href="​https://​www.zeiss.com/​microscopy/​en/​products/​light-microscopes/​widefield-microscopes/​axio-imager-2-for-life-science-research.html">​Zeiss Axio Imager 2 with Apotome 2</​a><​br>​
 <​b>​Nickname</​b>:​ Apotome<​br>​ <​b>​Nickname</​b>:​ Apotome<​br>​
-<​b>​Software</​b>: ​Zen 3.1<br>+<​b>​Software</​b>: ​ZEN 3.13<br>
 <​b>​Year</​b>: ​ 2019<​br>​ <​b>​Year</​b>: ​ 2019<​br>​
 <​b>​SN</​b>:​ 3523001603<​br>​ <​b>​SN</​b>:​ 3523001603<​br>​
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 ^  Filterset ​ ^  Excitation ​ ^  Dichroic ​ ^  Emission ​ ^  Reference ​ ^ ^  Filterset ​ ^  Excitation ​ ^  Dichroic ​ ^  Emission ​ ^  Reference ​ ^
-|  Blue  |  370-410 nm  |  420 nm  |  <fc #​0046ff>​430-470 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​shop/​filterAssistant/​filtersets/​489096-9100-000|FS96HE]] ​ | +|  Blue  |  370-410 nm  |  420 nm  |  <fc #​0046ff>​430-470 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​fluorescenceAssistant/​filtersets/​489096-9100-400|FS96HE]] ​ | 
-|  Green  |  450-490 nm  |  493 nm  |  <fc #​4aff00>​500-550 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​?​s=7139069fdc79c&​l=en&p=us&​f=f&​a=v&​b=f&​id=489038-9901-000&o=|FS38HE]] ​ | +|  Green  |  450-490 nm  |  493 nm  |  <fc #​4aff00>​500-550 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/us/​fluorescenceAssistant/​filtersets/​489038-9901-400|FS38HE]] ​ | 
-|  Orange-Red ​ |  540-552 nm  |  580 nm  |  <fc #​ffdf00>>​ 590 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​shop/​filterAssistant/​filtersets/​488015-0000-000|FS15]] ​ | +|  Orange-Red ​ |  540-552 nm  |  580 nm  |  <fc #​ffdf00>>​ 590 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​fluorescenceAssistant/​filtersets/​488015-0000-400|FS15]] ​ | 
-|  Red  |  625-655 nm  |  660 nm  |  <fc #​ff0000>​665-715 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​shop/​filterAssistant/​filtersets/​488050-9901-000|FS50]] ​ |+|  Red  |  625-655 nm  |  660 nm  |  <fc #​ff0000>​665-715 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​fluorescenceAssistant/​filtersets/​488050-9901-400|FS50]] ​ |
 |  DIC  |   ​- ​  ​| ​  ​- ​  ​| ​  ​- ​  ​| ​  ​- ​  | |  DIC  |   ​- ​  ​| ​  ​- ​  ​| ​  ​- ​  ​| ​  ​- ​  |
-|  Orange ​ |  575-600 nm  |  605 nm  |  <fc #​ff5300>​612-682 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​shop/​filterAssistant/​filtersets/​489064-0000-000|FS64HE]] ​ | +|  Orange ​ |  575-600 nm  |  605 nm  |  <fc #​ff5300>​612-682 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​fluorescenceAssistant/​filtersets/​489064-0000-400|FS64HE]] ​ | 
-|  QUAD  |  370-400 nm  |  405 nm  |  <fc #​5400ff>​410-440 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​shop/​filterAssistant/​filtersets/​489090-9100-000|  FS90HE]] ​ |+|  QUAD  |  370-400 nm  |  405 nm  |  <fc #​5400ff>​410-440 nm</​fc> ​ |  [[https://​www.micro-shop.zeiss.com/​en/​us/​fluorescenceAssistant/​filtersets/​489090-9110-400|  FS90HE]] ​ |
  
 ==== Objectives ==== ==== Objectives ====
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   * Turn on the Microscope Body   * Turn on the Microscope Body
 {{::​oeiras_apotome_turningon2.jpg?​200|}} {{::​oeiras_apotome_turningon2.jpg?​200|}}
-  * Turn on the computer ​(if needed)+  * Turn on the computer ​
   * Log In into Windows with your Agendo credentials   * Log In into Windows with your Agendo credentials
   * Start the **ZEN** software   * Start the **ZEN** software
  
 ===== Microscope Turn Off Procedure ===== ===== Microscope Turn Off Procedure =====
 +
 +If there is another user for this microscope in the next hour:
 +
 +  * Exit the **ZEN** software
 +  * Log off the computer
 +  * Clean up immersion objectives
 +
 +Else:
  
   * Exit the **ZEN** software   * Exit the **ZEN** software
-  * **Log off** the computer+  * Clean up immersion objectives
   * Turn off the Microscope Body   * Turn off the Microscope Body
   * Turn off the Power Supply   * Turn off the Power Supply
 +  * Turn off the computer
  
 ---- ----
 [[resources|Back to the Equipment page]] [[resources|Back to the Equipment page]]
zeiss_apotome.1765535782.txt.gz · Last modified: 2025/12/12 11:36 by bioimaging