zeiss_apotome
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zeiss_apotome [2025/12/12 11:33] bioimaging |
zeiss_apotome [2025/12/12 11:36] (current) bioimaging |
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The ZEISS Axio Imager 2 with Apotome 2 is a fully automated upright microscope capable of magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS color camera ([[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|Axiocam 105 RGB]], for brightfield/DIC) and on the top port a high sensitivity 4 Mpx monochrome sCMOS camera ([[https://www.biomedical-instruments-trading.com/products/video-devices/568-hamamatsu-orca-flash-4-0-v2|Hamamatsu Flash 4.0v2]], for fluorescence). The system has a Colibri 7 excitation light source with 5 LEDs (see table below). The system’s stage, which can accommodate 8 slides simultaneously, is motorized in XYZ, capable of z-stacks, multiple positions or tile and stitching acquisition (thereby allowing acquisition of large fields of view with several hundred megapixels). | + | The ZEISS Axio Imager with Apotome 2 is a fully automated upright microscope capable of magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS color camera ([[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|Axiocam 105 RGB]], for brightfield/DIC) and on the top port a high sensitivity 4 Mpx monochrome sCMOS camera ([[https://www.biomedical-instruments-trading.com/products/video-devices/568-hamamatsu-orca-flash-4-0-v2|Hamamatsu Flash 4.0v2]], for fluorescence). The system has a Colibri 7 excitation light source with 5 LEDs (see table below). The system’s stage, which can accommodate 8 slides simultaneously, is motorized in XYZ, capable of z-stacks, multiple positions or tile and stitching acquisition (thereby allowing acquisition of large fields of view with several hundred megapixels). |
| The Imager is also equipped with ApoTome.2, which allows optical sectioning of fluorescent samples using structured illumination. A grid pattern is placed on the illumination path and is projected and shifted sideways; 3 (or more) images are acquired and between each image the grid is moved 1/3 of its cycle. This movement generates images where only the in-focus light is modulated by the grid pattern while out-of-focus light is largely unaffected by the grid. By subtracting pairs of these images, the system removes out-of-focus light and the grid pattern, thus creating an optical section of the sample. | The Imager is also equipped with ApoTome.2, which allows optical sectioning of fluorescent samples using structured illumination. A grid pattern is placed on the illumination path and is projected and shifted sideways; 3 (or more) images are acquired and between each image the grid is moved 1/3 of its cycle. This movement generates images where only the in-focus light is modulated by the grid pattern while out-of-focus light is largely unaffected by the grid. By subtracting pairs of these images, the system removes out-of-focus light and the grid pattern, thus creating an optical section of the sample. | ||
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| * Turn on the computer (if needed) | * Turn on the computer (if needed) | ||
| * Log In into Windows with your Agendo credentials | * Log In into Windows with your Agendo credentials | ||
| - | * Start the **Zen** software | + | * Start the **ZEN** software |
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| - | * Exit the **Zen** software | + | * Exit the **ZEN** software |
| * **Log off** the computer | * **Log off** the computer | ||
| * Turn off the Microscope Body | * Turn off the Microscope Body | ||
zeiss_apotome.1765535594.txt.gz · Last modified: 2025/12/12 11:33 by bioimaging
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