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zeiss_apotome [2025/12/12 10:46] bioimaging |
zeiss_apotome [2026/04/17 23:46] (current) bioimaging |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=imager.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=imager.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=imager.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=imager.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B02<br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_apotome.png">[Oeiras] Room 0B02</a><br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html">Zeiss Axio Imager 2 with Apotome 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html">Zeiss Axio Imager 2 with Apotome 2</a><br> | ||
| <b>Nickname</b>: Apotome<br> | <b>Nickname</b>: Apotome<br> | ||
| - | <b>Software</b>: Zen 3.1<br> | + | <b>Software</b>: ZEN 3.13<br> |
| <b>Year</b>: 2019<br> | <b>Year</b>: 2019<br> | ||
| <b>SN</b>: 3523001603<br> | <b>SN</b>: 3523001603<br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The ZEISS Axio Imager 2 with Apotome 2 is a fully automated upright microscope capable of magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS color camera ([[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|Axiocam 105 RGB]], for brightfield/DIC) and on the top port a high sensitivity 4 Mpx monochrome sCMOS camera ([[https://www.biomedical-instruments-trading.com/products/video-devices/568-hamamatsu-orca-flash-4-0-v2|Hamamatsu Flash 4.0v2]], for fluorescence). The system has a Colibri 7 excitation light source with 5 LEDs (see table below). The system’s stage, which can accommodate 8 slides simultaneously, is motorized in XYZ, capable of z-stacks, multiple positions or tile and stitching acquisition (thereby allowing acquisition of large fields of view with several hundred megapixels). | + | The ZEISS Axio Imager with Apotome 2 is a fully automated upright microscope capable of magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS color camera ([[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|Axiocam 105 RGB]], for brightfield/DIC) and on the top port a high sensitivity 4 Mpx monochrome sCMOS camera ([[https://www.biomedical-instruments-trading.com/products/video-devices/568-hamamatsu-orca-flash-4-0-v2|Hamamatsu Flash 4.0v2]], for fluorescence). The system has a Colibri 7 excitation light source with 5 LEDs (see table below). The system’s stage, which can accommodate 8 slides simultaneously, is motorized in XYZ, capable of z-stacks, multiple positions or tile and stitching acquisition (thereby allowing acquisition of large fields of view with several hundred megapixels). |
| The Imager is also equipped with ApoTome.2, which allows optical sectioning of fluorescent samples using structured illumination. A grid pattern is placed on the illumination path and is projected and shifted sideways; 3 (or more) images are acquired and between each image the grid is moved 1/3 of its cycle. This movement generates images where only the in-focus light is modulated by the grid pattern while out-of-focus light is largely unaffected by the grid. By subtracting pairs of these images, the system removes out-of-focus light and the grid pattern, thus creating an optical section of the sample. | The Imager is also equipped with ApoTome.2, which allows optical sectioning of fluorescent samples using structured illumination. A grid pattern is placed on the illumination path and is projected and shifted sideways; 3 (or more) images are acquired and between each image the grid is moved 1/3 of its cycle. This movement generates images where only the in-focus light is modulated by the grid pattern while out-of-focus light is largely unaffected by the grid. By subtracting pairs of these images, the system removes out-of-focus light and the grid pattern, thus creating an optical section of the sample. | ||
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| ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| - | | Blue | 370-410 nm | 420 nm | <fc #0046ff>430-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489096-9100-000|FS96HE]] | | + | | Blue | 370-410 nm | 420 nm | <fc #0046ff>430-470 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489096-9100-400|FS96HE]] | |
| - | | Green | 450-490 nm | 493 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/?s=7139069fdc79c&l=en&p=us&f=f&a=v&b=f&id=489038-9901-000&o=|FS38HE]] | | + | | Green | 450-490 nm | 493 nm | <fc #4aff00>500-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489038-9901-400|FS38HE]] | |
| - | | Orange-Red | 540-552 nm | 580 nm | <fc #ffdf00>> 590 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/488015-0000-000|FS15]] | | + | | Orange-Red | 540-552 nm | 580 nm | <fc #ffdf00>> 590 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488015-0000-400|FS15]] | |
| - | | Red | 625-655 nm | 660 nm | <fc #ff0000>665-715 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/488050-9901-000|FS50]] | | + | | Red | 625-655 nm | 660 nm | <fc #ff0000>665-715 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488050-9901-400|FS50]] | |
| | DIC | - | - | - | - | | | DIC | - | - | - | - | | ||
| - | | Orange | 575-600 nm | 605 nm | <fc #ff5300>612-682 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489064-0000-000|FS64HE]] | | + | | Orange | 575-600 nm | 605 nm | <fc #ff5300>612-682 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489064-0000-400|FS64HE]] | |
| - | | QUAD | 370-400 nm | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/shop/filterAssistant/filtersets/489090-9100-000| FS90HE]] | | + | | QUAD | 370-400 nm | 405 nm | <fc #5400ff>410-440 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/489090-9110-400| FS90HE]] | |
| ==== Objectives ==== | ==== Objectives ==== | ||
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| * Turn on the Microscope Body | * Turn on the Microscope Body | ||
| {{::oeiras_apotome_turningon2.jpg?200|}} | {{::oeiras_apotome_turningon2.jpg?200|}} | ||
| - | * Turn on the computer (if needed) | + | * Turn on the computer |
| * Log In into Windows with your Agendo credentials | * Log In into Windows with your Agendo credentials | ||
| - | * Start the **Zen** software | + | * Start the **ZEN** software |
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| - | * Exit the **Zen** software | + | If there is another user for this microscope in the next hour: |
| - | * **Log off** the computer | + | |
| + | * Exit the **ZEN** software | ||
| + | * Log off the computer | ||
| + | * Clean up immersion objectives | ||
| + | |||
| + | Else: | ||
| + | |||
| + | * Exit the **ZEN** software | ||
| + | * Clean up immersion objectives | ||
| * Turn off the Microscope Body | * Turn off the Microscope Body | ||
| * Turn off the Power Supply | * Turn off the Power Supply | ||
| + | * Turn off the computer | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_apotome.1765532773.txt.gz · Last modified: 2025/12/12 10:46 by bioimaging
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