zeiss_apotome
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zeiss_apotome [2025/12/12 10:45] bioimaging |
zeiss_apotome [2025/12/12 11:36] (current) bioimaging |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=imager.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=imager.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=imager.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=imager.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B02<br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_apotome.png">[Oeiras] Room 0B02</a><br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html">Zeiss Axio Imager 2 with Apotome 2</a><br> | <b>Model</b>: <a href="https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-imager-2-for-life-science-research.html">Zeiss Axio Imager 2 with Apotome 2</a><br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The ZEISS Axio Imager 2 with Apotome 2 is a fully automated upright microscope capable of magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS color camera ([[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|Axiocam 105 RGB]], for brightfield/DIC) and on the top port a high sensitivity 4 Mpx monochrome sCMOS camera ([[https://www.biomedical-instruments-trading.com/products/video-devices/568-hamamatsu-orca-flash-4-0-v2|Hamamatsu Flash 4.0v2]], for fluorescence). The system has a Colibri 7 excitation light source with 5 LEDs (see table below). The system’s stage, which can accommodate 8 slides simultaneously, is motorized in XYZ, capable of z-stacks, multiple positions or tile and stitching acquisition (thereby allowing acquisition of large fields of view with several hundred megapixels). | + | The ZEISS Axio Imager with Apotome 2 is a fully automated upright microscope capable of magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS color camera ([[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|Axiocam 105 RGB]], for brightfield/DIC) and on the top port a high sensitivity 4 Mpx monochrome sCMOS camera ([[https://www.biomedical-instruments-trading.com/products/video-devices/568-hamamatsu-orca-flash-4-0-v2|Hamamatsu Flash 4.0v2]], for fluorescence). The system has a Colibri 7 excitation light source with 5 LEDs (see table below). The system’s stage, which can accommodate 8 slides simultaneously, is motorized in XYZ, capable of z-stacks, multiple positions or tile and stitching acquisition (thereby allowing acquisition of large fields of view with several hundred megapixels). |
| The Imager is also equipped with ApoTome.2, which allows optical sectioning of fluorescent samples using structured illumination. A grid pattern is placed on the illumination path and is projected and shifted sideways; 3 (or more) images are acquired and between each image the grid is moved 1/3 of its cycle. This movement generates images where only the in-focus light is modulated by the grid pattern while out-of-focus light is largely unaffected by the grid. By subtracting pairs of these images, the system removes out-of-focus light and the grid pattern, thus creating an optical section of the sample. | The Imager is also equipped with ApoTome.2, which allows optical sectioning of fluorescent samples using structured illumination. A grid pattern is placed on the illumination path and is projected and shifted sideways; 3 (or more) images are acquired and between each image the grid is moved 1/3 of its cycle. This movement generates images where only the in-focus light is modulated by the grid pattern while out-of-focus light is largely unaffected by the grid. By subtracting pairs of these images, the system removes out-of-focus light and the grid pattern, thus creating an optical section of the sample. | ||
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| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| - | * Turn on the ''Power Supply'' | + | * Turn on the Power Supply |
| - | {{::oeiras_apotome_turningon1.jpg?300|}} | + | {{::oeiras_apotome_turningon1.jpg?200|}} |
| - | * Turn on the ''Microscope Body'' | + | * Turn on the Microscope Body |
| - | {{::oeiras_apotome_turningon2.jpg?300|}} | + | {{::oeiras_apotome_turningon2.jpg?200|}} |
| * Turn on the computer (if needed) | * Turn on the computer (if needed) | ||
| * Log In into Windows with your Agendo credentials | * Log In into Windows with your Agendo credentials | ||
| - | * Start the **Zen** software | + | * Start the **ZEN** software |
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| - | * Exit the **Zen** software | + | * Exit the **ZEN** software |
| * **Log off** the computer | * **Log off** the computer | ||
| - | * Turn off the ''Microscope Body'' | + | * Turn off the Microscope Body |
| - | * Turn off the ''Power Supply'' | + | * Turn off the Power Supply |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
zeiss_apotome.1765532736.txt.gz · Last modified: 2025/12/12 10:45 by bioimaging
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