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zeiss_apotome [2025/01/14 16:35] bioimaging [Microscope Turn On Procedure] |
zeiss_apotome [2025/07/19 21:29] (current) bioimaging |
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<b>Software</b>: Zen 3.1<br> | <b>Software</b>: Zen 3.1<br> | ||
<b>Year</b>: 2019<br> | <b>Year</b>: 2019<br> | ||
- | <b>SN</b>: <br> | + | <b>SN</b>: 3523001603<br> |
  <br> |   <br> | ||
Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
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magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS | magnifications from 2.5x to 160x and equipped with two cameras: On the left port a 5MPx CMOS | ||
color camera ([[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|Axiocam 105 RGB]], for brightfield/DIC) and on the top port a high sensitivity 4Mpx | color camera ([[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|Axiocam 105 RGB]], for brightfield/DIC) and on the top port a high sensitivity 4Mpx | ||
- | monochrome sCMOS camera (Hamamatsu Flash 4.0v2, for fluorescence). The system has a | + | monochrome sCMOS camera ([[https://www.biomedical-instruments-trading.com/products/video-devices/568-hamamatsu-orca-flash-4-0-v2|Hamamatsu Flash 4.0v2]], for fluorescence). The system has a |
Colibri7 excitation light source with 5 LEDs (see table below). The system’s stage, which can | Colibri7 excitation light source with 5 LEDs (see table below). The system’s stage, which can | ||
accommodate 8 slides simultaneously, is motorized in XYZ, capable of z-stacks, multiple | accommodate 8 slides simultaneously, is motorized in XYZ, capable of z-stacks, multiple | ||
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==== Camera ==== | ==== Camera ==== | ||
+ | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
+ | | [[https://www.biomedical-instruments-trading.com/products/video-devices/568-hamamatsu-orca-flash-4-0-v2|Hamamatsu ORCA-Flash4.0 V2]] | 2048 x 2048 | 13.3 x 13.3 | 70 % | | ||
+ | | [[https://www.zeiss.com/microscopy/en/products/cameras/axiocam-105-color.html|ZEISS Axiocam 105 color]] | 2048 x 2048 | 2.2 x 2.2 | - | | ||
===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
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* Turn on the ''Microscope Body'' | * Turn on the ''Microscope Body'' | ||
{{::oeiras_apotome_turningon2.jpg?300|}} | {{::oeiras_apotome_turningon2.jpg?300|}} | ||
- | * Turn on the computer | + | * Turn on the computer (if needed) |
* Log In into Windows with your Agendo credentials | * Log In into Windows with your Agendo credentials | ||
* Start the **Zen** software | * Start the **Zen** software | ||
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* Exit the **Zen** software | * Exit the **Zen** software | ||
- | * Log off from the computer | + | * **Log off** the computer |
* Turn off the ''Microscope Body'' | * Turn off the ''Microscope Body'' | ||
* Turn off the ''Power Supply'' | * Turn off the ''Power Supply'' |