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oeiras_zeiss_lumar_v12
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oeiras_zeiss_lumar_v12 [2025/04/04 17:03] bioimaging |
oeiras_zeiss_lumar_v12 [2026/08/11 03:04] (current) bioimaging |
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| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=lumar.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lumar.jpg" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=lumar.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lumar.jpg" width=350></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B03<br> | + | <b>Location</b>: Training Lab<br> |
| <b>Manufacturer</b>: ZEISS <br> | <b>Manufacturer</b>: ZEISS <br> | ||
| <b>Model</b>: Stereo Lumar V.12 <br> | <b>Model</b>: Stereo Lumar V.12 <br> | ||
| <b>Nickname</b>: Lumar <br> | <b>Nickname</b>: Lumar <br> | ||
| - | <b>Software</b>: Micromanager (Hamamatsu camera) / uEye (uEye camera) <br> | ||
| <b>Year</b>: 2011 <br> | <b>Year</b>: 2011 <br> | ||
| - | <b>SN</b>: <br> | + | <br> |
| - |   <br> | + | <br> |
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| + | <br> | ||
| Data will be deleted after: <b>3 months</b> | Data will be deleted after: <b>3 months</b> | ||
|   <br> |   <br> | ||
| - |   <br> | + | → <a href="/facility/bioimaging/doku.php?id=oeiras_lumar_quality"><img src="/facility/bioimaging/lib/exe/fetch.php?media=list-icon.png" width=18> ZEISS Stereo Lumar.V12 Quality Control</a> |
| - |   <br> | + | <br> → <a href="/facility/bioimaging/doku.php?id=oeiras_lumar_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> ZEISS Stereo Lumar.V12 Usage Statistics</a> |
| - | → <a href="/facility/bioimaging/doku.php?id=oeiras_lumar_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> ZEISS Stereo Lumra.V12 Usage Statistics</a> | + | |
| </p> | </p> | ||
| </td> | </td> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The Stereo Lumar V.12 is a motorized stereoscope with fluorescence, capable of imaging in colour and grayscale (camera dependent), with a zoom between 9.6x and 120x. | + | The Stereo Lumar V.12 is a motorized stereoscope with fluorescence, with a zoom between 9.6x and 120x. |
| ===== System components ===== | ===== System components ===== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ==== Filtersets ==== |
| - | | 1 | CFP | Filterset 47 | 436/20 | 455 | 480/40 | | + | |
| - | | 2 | GFP | Filterset 13 | 470/20 | 495 | 505-530 | | + | |
| - | | 3 | TexasRed | Filterset 45 | 560/40 | 585 | 630/75 | | + | |
| - | | 4 | YFP | Filterset 46 | 500/20 | 515 | 535/30 | | + | |
| + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | 1 | Cyan | 426-446 nm | 455 nm | <fc #00d5ff>460-500 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488047-9100-400|FS47]] | | ||
| + | | 2 | Green | 460-490 nm | 495 nm | <fc #0fff00>505-530 nm</fc> | FS13 | | ||
| + | | 3 | Red | 540-580 nm | 585 nm | <fc #ff4f00>592-667 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488045-9100-400|FS45]] | | ||
| + | | 4 | Yellow | 490-510 nm | 515 nm | <fc #a3ff00>520-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/000000-1196-681|FS46]] | | ||
| - | ===== Camera ===== | + | ===== Microscope Turn On Procedure ===== |
| - | Camera can be changed on request, and training for this can be provided if needed. | ||
| - | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| - | | [[https://en.ids-imaging.com/store/ui-3370cp-rev-2.html|UI-3370CP Rev. 2 Colour]] | 2048 × 2048 | 5.5 x 5.5 | - | | ||
| - | | [[https://hep.hamamatsu.com/eu/en/products/C11440-42U30.html|ORCA-Flash4.0 LT+ Monochrome]] | 2048 × 2048 | 6.5 x 6.5 | 82% | | ||
| - | |||
| - | ===== Pixel-size vs magnification ===== | ||
| - | |||
| - | ^ Magnification ^ Hamamatsu (µm/pixel) ^ IDS (µm/pixel) ^ | ||
| - | | 9.6x | 6.10 | 9.40 | | ||
| - | | 10.0x | 5.86 | 9.02 | | ||
| - | | 20.0x | 2.92 | 4.52 | | ||
| - | | 30.0x | 1.94 | 3.02 | | ||
| - | | 40.0x | 1.45 | 2.26 | | ||
| - | | 50.0x | 1.16 | 1.81 | | ||
| - | | 60.0x | 0.97 | 1.51 | | ||
| - | | 70.0x | 0.83 | 1.30 | | ||
| - | | 80.0x | 0.72 | 1.13 | | ||
| - | | 90.0x | 0.64 | 1.01 | | ||
| - | | 100.0x | 0.58 | 0.91 | | ||
| - | | 110.0x | 0.53 | 0.83 | | ||
| - | | 120.0x | 0.48 | 0.76 | | ||
| - | |||
| - | |||
| - | ===== Microscope Turn On Procedure ===== | ||
| * Turn on the **powerbar below the stone table**. | * Turn on the **powerbar below the stone table**. | ||
| * **If fluorescence is necessary**, turn on the **fluorescent lamp controller** on top of the desk. | * **If fluorescence is necessary**, turn on the **fluorescent lamp controller** on top of the desk. | ||
| + | |||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| + | |||
| * Turn on the **powerbar below the stone table**. | * Turn on the **powerbar below the stone table**. | ||
| * **If fluorescence was used**, turn off the **fluorescent lamp controller** on top of the desk. | * **If fluorescence was used**, turn off the **fluorescent lamp controller** on top of the desk. | ||
oeiras_zeiss_lumar_v12.1743779010.txt.gz · Last modified: 2025/04/04 17:03 by bioimaging
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