oeiras_zeiss_lumar_v12
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oeiras_zeiss_lumar_v12 [2025/01/06 01:14] bioimaging |
oeiras_zeiss_lumar_v12 [2025/04/04 19:10] (current) bioimaging [Pixel-size vs magnification] |
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| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| <b>Location</b>: [Oeiras] Room 0B03<br> | <b>Location</b>: [Oeiras] Room 0B03<br> | ||
| - | <b>Manufacturer</b>: <br> | + | <b>Manufacturer</b>: ZEISS <br> |
| - | <b>Model</b>: <br> | + | <b>Model</b>: Stereo Lumar V.12 <br> |
| - | <b>Nickname</b>: <br> | + | <b>Nickname</b>: Lumar <br> |
| - | <b>Software</b>: <br> | + | <b>Software</b>: Micromanager (Hamamatsu camera) / uEye (uEye camera) <br> |
| - | <b>Year</b>: <br> | + | <b>Year</b>: 2011 <br> |
| <b>SN</b>: <br> | <b>SN</b>: <br> | ||
|   <br> |   <br> | ||
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| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| + | |||
| + | The Stereo Lumar V.12 is a motorized stereoscope with fluorescence, capable of imaging in colour and grayscale (camera dependent), with a zoom between 9.6x and 120x. | ||
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| ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | ||
| - | | | | | | | | | + | | 1 | CFP | Filterset 47 | 436/20 | 455 | 480/40 | |
| + | | 2 | GFP | Filterset 13 | 470/20 | 495 | 505-530 | | ||
| + | | 3 | TexasRed | Filterset 45 | 560/40 | 585 | 630/75 | | ||
| + | | 4 | YFP | Filterset 46 | 500/20 | 515 | 535/30 | | ||
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| + | |||
| + | ===== Camera ===== | ||
| + | |||
| + | Camera can be changed on request, and training for this can be provided if needed. | ||
| + | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| + | | [[https://en.ids-imaging.com/store/ui-3370cp-rev-2.html|UI-3370CP Rev. 2 Colour]] | 2048 × 2048 | 5.5 x 5.5 | - | | ||
| + | | [[https://hep.hamamatsu.com/eu/en/products/C11440-42U30.html|ORCA-Flash4.0 LT+ Monochrome]] | 2048 × 2048 | 6.5 x 6.5 | 82% | | ||
| + | |||
| + | ===== Pixel-size vs magnification ===== | ||
| + | |||
| + | ^ Magnification ^ Hamamatsu (µm/pixel) ^ IDS (µm/pixel) ^ | ||
| + | | 9.6x | 10.90 | 9.40 | | ||
| + | | 10.0x | 10.49 | 9.02 | | ||
| + | | 20.0x | 5.07 | 4.52 | | ||
| + | | 30.0x | 3.41 | 3.02 | | ||
| + | | 40.0x | 2.59 | 2.26 | | ||
| + | | 50.0x | 2.05 | 1.81 | | ||
| + | | 60.0x | 1.73 | 1.51 | | ||
| + | | 70.0x | 1.49 | 1.30 | | ||
| + | | 80.0x | 1.29 | 1.13 | | ||
| + | | 90.0x | 1.16 | 1.01 | | ||
| + | | 100.0x | 1.04 | 0.91 | | ||
| + | | 110.0x | 0.95 | 0.83 | | ||
| + | | 120.0x | 0.87 | 0.76 | | ||
| - | ==== Objectives ==== | + | ===== Microscope Turn On Procedure ===== |
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | + | * Turn on the **powerbar below the stone table**. |
| - | | | | | | | | + | * **If fluorescence is necessary**, turn on the **fluorescent lamp controller** on top of the desk. |
| + | ===== Microscope Turn Off Procedure ===== | ||
| + | * Turn on the **powerbar below the stone table**. | ||
| + | * **If fluorescence was used**, turn off the **fluorescent lamp controller** on top of the desk. | ||
| + | * This lamp **has to be on for 30 minutes**. If you use the system less than 30 minutes, **wait** until that time to turn the lamp off, or **warn the Bioimaging Team**. | ||
| - | ==== Camera ==== | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
oeiras_zeiss_lumar_v12.1736122455.txt.gz · Last modified: 2025/01/06 01:14 by bioimaging
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