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oeiras_zeiss_lumar_v12
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oeiras_zeiss_lumar_v12 [2026/02/17 17:51] bioimaging |
oeiras_zeiss_lumar_v12 [2026/02/17 18:02] (current) bioimaging |
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| ===== System components ===== | ===== System components ===== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ==== Filtersets ==== |
| - | | 1 | CFP | Filterset 47 | 436/20 | 455 | 480/40 | | + | |
| - | | 2 | GFP | Filterset 13 | 470/20 | 495 | 505-530 | | + | |
| - | | 3 | TexasRed | Filterset 45 | 560/40 | 585 | 630/75 | | + | |
| - | | 4 | YFP | Filterset 46 | 500/20 | 515 | 535/30 | | + | |
| + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ | ||
| + | | 1 | Cyan | 426-446 nm | 455 nm | <fc #00d5ff>460-500 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488047-9100-400|FS47]] | | ||
| + | | 2 | Green | 460-490 nm | 495 nm | <fc #0fff00>505-530 nm</fc> | FS13 | | ||
| + | | 3 | Red | 540-580 nm | 585 nm | <fc #ff4f00>592-667 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488045-9100-400|FS45]] | | ||
| + | | 4 | Yellow | 490-510 nm | 515 nm | <fc #a3ff00>520-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/000000-1196-681|FS46]] | | ||
| - | ===== Camera ===== | + | ==== Camera ==== |
| Camera can be changed on request, and training for this can be provided if needed. | Camera can be changed on request, and training for this can be provided if needed. | ||
| Line 46: | Line 47: | ||
| | ORCA-Flash4.0 - C11440-22C | 2048 × 2048 | 6.5 x 6.5 | 82% | After May 5, 2025 | | | ORCA-Flash4.0 - C11440-22C | 2048 × 2048 | 6.5 x 6.5 | 82% | After May 5, 2025 | | ||
| - | ===== Pixel-size vs magnification ===== | + | ==== Pixel-size vs magnification ==== |
| ^ Magnification ^ Hamamatsu (µm/pixel) ^ IDS (µm/pixel) ^ | ^ Magnification ^ Hamamatsu (µm/pixel) ^ IDS (µm/pixel) ^ | ||
| Line 65: | Line 66: | ||
| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||
| + | |||
| * Turn on the **powerbar below the stone table**. | * Turn on the **powerbar below the stone table**. | ||
| * **If fluorescence is necessary**, turn on the **fluorescent lamp controller** on top of the desk. | * **If fluorescence is necessary**, turn on the **fluorescent lamp controller** on top of the desk. | ||
| + | |||
| ===== Microscope Turn Off Procedure ===== | ===== Microscope Turn Off Procedure ===== | ||
| + | |||
| * Turn on the **powerbar below the stone table**. | * Turn on the **powerbar below the stone table**. | ||
| * **If fluorescence was used**, turn off the **fluorescent lamp controller** on top of the desk. | * **If fluorescence was used**, turn off the **fluorescent lamp controller** on top of the desk. | ||
oeiras_zeiss_lumar_v12.txt · Last modified: 2026/02/17 18:02 by bioimaging
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