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oeiras_zeiss_lumar_v12 [2026/08/11 03:02] bioimaging [Microscope overview] |
oeiras_zeiss_lumar_v12 [2026/08/11 03:04] (current) bioimaging |
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| | 3 | Red | 540-580 nm | 585 nm | <fc #ff4f00>592-667 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488045-9100-400|FS45]] | | | 3 | Red | 540-580 nm | 585 nm | <fc #ff4f00>592-667 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/488045-9100-400|FS45]] | | ||
| | 4 | Yellow | 490-510 nm | 515 nm | <fc #a3ff00>520-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/000000-1196-681|FS46]] | | | 4 | Yellow | 490-510 nm | 515 nm | <fc #a3ff00>520-550 nm</fc> | [[https://www.micro-shop.zeiss.com/en/us/fluorescenceAssistant/filtersets/000000-1196-681|FS46]] | | ||
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| - | ==== Camera ==== | ||
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| - | Camera can be changed on request, and training for this can be provided if needed. | ||
| - | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ Installed date ^ | ||
| - | | [[https://en.ids-imaging.com/store/ui-3370cp-rev-2.html|UI-3370CP Rev. 2]] Color Camera | 2048 × 2048 | 5.5 x 5.5 | - | Since Oct 2024 | | ||
| - | | [[https://web.archive.org/web/20241014073627/https://hep.hamamatsu.com/eu/en/products/C11440-42U30.html|ORCA-Flash4.0 LT+]] | 2048 × 2048 | 6.5 x 6.5 | 82% | Before May 5, 2025 | | ||
| - | | ORCA-Flash4.0 - C11440-22C | 2048 × 2048 | 6.5 x 6.5 | 82% | After May 5, 2025 | | ||
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| - | ==== Pixel-size vs magnification ==== | ||
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| - | ^ Magnification ^ Hamamatsu (µm/pixel) ^ IDS (µm/pixel) ^ | ||
| - | | 9.6x | 10.90 | 9.40 | | ||
| - | | 10.0x | 10.49 | 9.02 | | ||
| - | | 20.0x | 5.07 | 4.52 | | ||
| - | | 30.0x | 3.41 | 3.02 | | ||
| - | | 40.0x | 2.59 | 2.26 | | ||
| - | | 50.0x | 2.05 | 1.81 | | ||
| - | | 60.0x | 1.73 | 1.51 | | ||
| - | | 70.0x | 1.49 | 1.30 | | ||
| - | | 80.0x | 1.29 | 1.13 | | ||
| - | | 90.0x | 1.16 | 1.01 | | ||
| - | | 100.0x | 1.04 | 0.91 | | ||
| - | | 110.0x | 0.95 | 0.83 | | ||
| - | | 120.0x | 0.87 | 0.76 | | ||
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| ===== Microscope Turn On Procedure ===== | ===== Microscope Turn On Procedure ===== | ||