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oeiras_zeiss_lightsheet_z1
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oeiras_zeiss_lightsheet_z1 [2025/01/06 00:54] bioimaging |
oeiras_zeiss_lightsheet_z1 [2026/02/17 17:46] (current) bioimaging [Filtersets] |
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| <a href="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg" width=300></a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg" width=300></a> | ||
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: [Oeiras] Room 0B03<br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=bartolomeu_dias_wing_2025_-_z1.png">[Oeiras] Room 0B03</a><br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | ||
| <b>Nickname</b>: "Lightsheet"<br> | <b>Nickname</b>: "Lightsheet"<br> | ||
| - | <b>Software</b>: ZEN 2014 SP1 v9.0.0.0 <br> | + | <b>Software</b>: ZEN 3.1 LS (HF 16)<br> |
| <b>Year</b>: 2020 <br> | <b>Year</b>: 2020 <br> | ||
| <b>SN</b>: 2583000325 <br> | <b>SN</b>: 2583000325 <br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lightsheet Z.1 Usage Statistics</a> | + | → <a href="/facility/bioimaging/doku.php?id=oeiras_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> ZEISS Lightsheet Z.1 Usage Statistics</a> |
| </p> | </p> | ||
| </td> | </td> | ||
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| ==== LASERs ==== | ==== LASERs ==== | ||
| - | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | + | |
| - | | Solid State 445| 445 nm | 20 mW | | + | ^ LASER ^ Wavelength ^ Maximum Power ^ |
| - | | Solid State 488 | 488 nm | 50 mW | | + | | Blue-Violet | <fc #0028ff>445 nm</fc> | 20 mW | |
| - | | Solid State 561 | 561 nm | 50 mW | | + | | Blue | <fc #00f7ff>488 nm</fc> | 50 mW | |
| - | | Solid State 638 | 638 nm | 75 mW | | + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 50 mW | |
| + | | Red | <fc #ff2600>638 nm</fc> | 75 mW | | ||
| ==== Objectives (Illumination) ==== | ==== Objectives (Illumination) ==== | ||
| ^ Magnification ^ Model ^ NA ^ | ^ Magnification ^ Model ^ NA ^ | ||
| - | | 10x | Lightsheet Z.1 10x | 0.20 | | + | | <fc #fef042>10x</fc> | Lightsheet Z.1 10x | 0.20 | |
| ==== Objectives (Detection) ==== | ==== Objectives (Detection) ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 20x | W Plan-Apochromat DIC 75mm | Water | 1.00 | 2.4 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421452-9700-000/Objective-W-Plan-Apochromat-20x-1.0-DIC-M27-75mm#|421452-9700-000]] | | + | | <fc #0aa05c>20x</fc> | W Plan-Apochromat DIC 75mm | Water | 1.00 | 2.4 | [[https://www.micro-shop.zeiss.com/en/de/shop/objectives/421452-9700-000/Objective-W-Plan-Apochromat-20x-1.0-DIC-M27-75mm#|421452-9700-000]] | |
| - | | 40x | W Plan-Apochromat DIC M27 | Water | 1.00 | 2.5 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/421462-9900-799/Objective-W-Plan-Apochromat-40x-1.0-DIC-M27| 421462-9900-799]] | | + | | <fc #01b3f5>40x</fc> | W Plan-Apochromat DIC M27 | Water | 1.00 | 2.5 | [[https://www.micro-shop.zeiss.com/en/us/shop/objectives/421462-9900-799/Objective-W-Plan-Apochromat-40x-1.0-DIC-M27| 421462-9900-799]] | |
| ==== Filtersets ==== | ==== Filtersets ==== | ||
| - | In each experiment you can only use Filter Turret A (installed by default) or B. Please let the facility staff know that you want to use filter turret B so that they can install and configure it in advance. | + | In each experiment you can only use Filter Turret A (installed by default) or B. Please let us know that you want to use filter turret B so that we can install and configure it in advance. |
| + | |||
| + | === Filter Turret A (default) === | ||
| - | The list of fluorophores presented in both tables is meant to be representative. Use [[https://www.thermofisher.com/order/fluorescence-spectraviewer/|Spectra Viewer]] or [[https://www.fpbase.org|FPBase]] to determine which set of lasers and emission filters you want to optimize the system to the specific fluorophores you have in your sample. | + | ^ Select Label ^ Camera #1 Filter ^ Camera #2 Filter ^ Fluorophores (Cam #1 : Cam #2) ^ |
| + | | LP 510 | <fc #00d5ff>460-500 nm</fc> | <fc #ffef00>> 585 nm</fc> | CFP, mCherry : RFP | | ||
| + | | LP 560 | <fc #4aff00>505-545 nm</fc> | <fc #ffef00>565-615 nm</fc> | GFP, mCherry : RFP | | ||
| + | | LP 560 (2) | <fc #4aff00>505-545 nm</fc> | <fc #ffef00>> 585 nm</fc> | GFP, mCherry : RFP | | ||
| + | | LP 560 (3) | <fc #4aff00>505-545 nm</fc> | <fc #ff0000>> 660 nm</fc> | GFP, mPlum : mStrawberry, tdTomato | | ||
| - | ^ Select Label ^ Camera #1 Filter ^ Camera #2 Filter ^ Fluorophores (Cam #1 : Cam #2) ^ Filter Turret ^ | + | === Filter Turret B === |
| - | | LP 510 | BP 460-500 | LP 585 | CFP, mCherry : RFP | A | | + | |
| - | | LP 560 | BP 505-545 | BP 565-615 | GFP, mCherry : RFP | A | | + | |
| - | | LP 560 (2) | BP 505-545 | LP 585 | GFP, mCherry : RFP | A | | + | |
| - | | LP 560 (3) | BP 505-545 | LP 660 | GFP, mPlum : mStrawberry, tdTomato | A | | + | |
| - | ^ Select Label ^ Camera #1 Filter ^ Camera #2 Filter ^ Fluorophores (Cam #1 : Cam #2) ^ Filter Turret ^ | + | ^ Select Label ^ Camera #1 Filter ^ Camera #2 Filter ^ Fluorophores (Cam #1 : Cam #2) ^ |
| - | | LP 510 | BP 460-500 | LP 660 | CFP, YFP : Cy5 | B | | + | | LP 510 | <fc #00d5ff>460-500 nm</fc> | <fc #ff0000>> 660 nm</fc> | CFP, YFP : Cy5 | |
| - | | LP 580 | BP 525-565 | LP 585 | YFP, mCherry : mPlum | B | | + | | LP 580 | <fc #92ff00>525-565 nm</fc> | <fc #ffef00>> 585 nm</fc> | YFP, mCherry : mPlum | |
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| * If the computer is already turned on, restart it. Start the system and incubator if you are using either CO2, humidity or/and Peltier modules. Check the humidifier bottle, and replace water if necessary. | * If the computer is already turned on, restart it. Start the system and incubator if you are using either CO2, humidity or/and Peltier modules. Check the humidifier bottle, and replace water if necessary. | ||
| - | * Start the Zen Black software (icon in Windows desktop), select the “start system” with the “boot status” option on, and confirm that no errors show up. | + | * Start the **ZEN** software (icon in Windows desktop), select the “start system” with the “boot status” option on, and confirm that no errors show up. |
| If you do not start the incubator system, you may receive errors relating to the absence of these systems in ZEN software; however the system should be working as expected. If this is not the case, please contact the facility staff. | If you do not start the incubator system, you may receive errors relating to the absence of these systems in ZEN software; however the system should be working as expected. If this is not the case, please contact the facility staff. | ||
oeiras_zeiss_lightsheet_z1.1736121264.txt.gz · Last modified: 2025/01/06 00:54 by bioimaging
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