oeiras_zeiss_lightsheet_z1
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oeiras_zeiss_lightsheet_z1 [2024/12/10 13:54] bioimaging |
oeiras_zeiss_lightsheet_z1 [2025/07/19 21:53] (current) bioimaging |
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| <html> | <html> | ||
| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_zeiss_lightsheet_z.1_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_zeiss_lightsheet_z1_wiki_2024.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_bioimagingplatform.png">Bartolomeu Dias Wing</a> | <a href="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_zeiss_lightsheet_z1_map_new_0B03.png">Room 0B03</a> </a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 4533) <br> | + | <b>Location</b>: [Oeiras] Room 0B03<br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | ||
| <b>Nickname</b>: "Lightsheet"<br> | <b>Nickname</b>: "Lightsheet"<br> | ||
| - | <b>Software</b>: ZEN 2014 SP1 v9.0.0.0 <br> | + | <b>Software</b>: ZEN 3.1 LS (HF 16)<br> |
| <b>Year</b>: 2020 <br> | <b>Year</b>: 2020 <br> | ||
| <b>SN</b>: 2583000325 <br> | <b>SN</b>: 2583000325 <br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lightsheet Z.1 Usage Statistics</a> | + | → <a href="/facility/bioimaging/doku.php?id=oeiras_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> ZEISS Lightsheet Z.1 Usage Statistics</a> |
| </p> | </p> | ||
| </td> | </td> | ||
| Line 38: | Line 38: | ||
| * **Sample Size**: up to 1 x 1 x 2 cm | * **Sample Size**: up to 1 x 1 x 2 cm | ||
| + | <html> | ||
| + | <!-- | ||
| * [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=en_poster_overview-clearing-methods_rel-2.0.pdf|Clearing Methods in Microscopy (poster)]] | * [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=en_poster_overview-clearing-methods_rel-2.0.pdf|Clearing Methods in Microscopy (poster)]] | ||
| - | * [[https://hcbi.fas.harvard.edu/files/lightsheetz1_sample-preparation_zeiss.pdf| Sample Preparation for Light Sheet Microscopy (white paper)]] | ||
| * [[https://asset-downloads.zeiss.com/catalogs/download/mic/b91bc689-66ab-4d85-b3f6-c31ebea22ba7/EN_wp_Lightsheet-Z.1_Expanding-Clearing-Solutions.pdf| Improved Imaging of Cleared Samples (application note)]] | * [[https://asset-downloads.zeiss.com/catalogs/download/mic/b91bc689-66ab-4d85-b3f6-c31ebea22ba7/EN_wp_Lightsheet-Z.1_Expanding-Clearing-Solutions.pdf| Improved Imaging of Cleared Samples (application note)]] | ||
| - | * [[https://www.youtube.com/watch?v=Ki1wtoXIkis|Sample Preparation for Whole Organisms, Plants, and 3D Cell & Tissue Cultures (video)]] | ||
| * [[https://www.youtube.com/watch?v=Ql7T7LrvI_I|ZEISS Microscopy How-to: Mount cleared samples in Lightsheet Z.1 (video)]] | * [[https://www.youtube.com/watch?v=Ql7T7LrvI_I|ZEISS Microscopy How-to: Mount cleared samples in Lightsheet Z.1 (video)]] | ||
| * [[https://www.youtube.com/watch?v=vKHQudCKVGc|ZEISS Webinar: In Vivo and Cleared Tissue Imaging with Light Sheet Fluorescence Microscopy (video)]] | * [[https://www.youtube.com/watch?v=vKHQudCKVGc|ZEISS Webinar: In Vivo and Cleared Tissue Imaging with Light Sheet Fluorescence Microscopy (video)]] | ||
| + | --> | ||
| + | </html> | ||
| + | |||
| + | * [[https://hcbi.fas.harvard.edu/files/lightsheetz1_sample-preparation_zeiss.pdf| Sample Preparation for Light Sheet Microscopy (white paper)]] | ||
| + | * [[https://www.youtube.com/watch?v=Ki1wtoXIkis|Sample Preparation for Whole Organisms, Plants, and 3D Cell & Tissue Cultures (video)]] | ||
| + | |||
| ===== Tutorials for Image Analysis with Arivis ===== | ===== Tutorials for Image Analysis with Arivis ===== | ||
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| ==== LASERs ==== | ==== LASERs ==== | ||
| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | Solid State 445| 445 nm | 20 mW | | + | | Solid State 445 | 445 nm | 20 mW | |
| | Solid State 488 | 488 nm | 50 mW | | | Solid State 488 | 488 nm | 50 mW | | ||
| - | | Solid State 561 | 561 nm | 50 mW | | + | | Solid State 561 | 561 nm | 50 mW | |
| | Solid State 638 | 638 nm | 75 mW | | | Solid State 638 | 638 nm | 75 mW | | ||
| Line 91: | Line 97: | ||
| * If the computer is already turned on, restart it. Start the system and incubator if you are using either CO2, humidity or/and Peltier modules. Check the humidifier bottle, and replace water if necessary. | * If the computer is already turned on, restart it. Start the system and incubator if you are using either CO2, humidity or/and Peltier modules. Check the humidifier bottle, and replace water if necessary. | ||
| - | * Start the Zen Black software (icon in Windows desktop), select the “start system” with the “boot status” option on, and confirm that no errors show up. | + | * Start the **ZEN** software (icon in Windows desktop), select the “start system” with the “boot status” option on, and confirm that no errors show up. |
| If you do not start the incubator system, you may receive errors relating to the absence of these systems in ZEN software; however the system should be working as expected. If this is not the case, please contact the facility staff. | If you do not start the incubator system, you may receive errors relating to the absence of these systems in ZEN software; however the system should be working as expected. If this is not the case, please contact the facility staff. | ||
oeiras_zeiss_lightsheet_z1.1733835263.txt.gz · Last modified: 2024/12/10 13:54 by bioimaging
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