oeiras_zeiss_lightsheet_z1
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This shows you the differences between two versions of the page.
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oeiras_zeiss_lightsheet_z1 [2024/12/09 14:54] bioimaging |
oeiras_zeiss_lightsheet_z1 [2025/03/14 11:01] (current) bioimaging |
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| <html> | <html> | ||
| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_zeiss_lightsheet_z.1_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a> Room 0B03 </a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 4533) <br> | + | <b>Location</b>: [Oeiras] Room 0B03<br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | ||
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|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Zeiss Lightsheet Z.1 Usage Statistics</a> | + | → <a href="/facility/bioimaging/doku.php?id=oeiras_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> ZEISS Lightsheet Z.1 Usage Statistics</a> |
| </p> | </p> | ||
| </td> | </td> | ||
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| * **Sample Size**: up to 1 x 1 x 2 cm | * **Sample Size**: up to 1 x 1 x 2 cm | ||
| + | <html> | ||
| + | <!-- | ||
| * [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=en_poster_overview-clearing-methods_rel-2.0.pdf|Clearing Methods in Microscopy (poster)]] | * [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=en_poster_overview-clearing-methods_rel-2.0.pdf|Clearing Methods in Microscopy (poster)]] | ||
| - | * [[https://hcbi.fas.harvard.edu/files/lightsheetz1_sample-preparation_zeiss.pdf| Sample Preparation for Light Sheet Microscopy (white paper)]] | ||
| * [[https://asset-downloads.zeiss.com/catalogs/download/mic/b91bc689-66ab-4d85-b3f6-c31ebea22ba7/EN_wp_Lightsheet-Z.1_Expanding-Clearing-Solutions.pdf| Improved Imaging of Cleared Samples (application note)]] | * [[https://asset-downloads.zeiss.com/catalogs/download/mic/b91bc689-66ab-4d85-b3f6-c31ebea22ba7/EN_wp_Lightsheet-Z.1_Expanding-Clearing-Solutions.pdf| Improved Imaging of Cleared Samples (application note)]] | ||
| - | * [[https://www.youtube.com/watch?v=Ki1wtoXIkis|Sample Preparation for Whole Organisms, Plants, and 3D Cell & Tissue Cultures (video)]] | ||
| * [[https://www.youtube.com/watch?v=Ql7T7LrvI_I|ZEISS Microscopy How-to: Mount cleared samples in Lightsheet Z.1 (video)]] | * [[https://www.youtube.com/watch?v=Ql7T7LrvI_I|ZEISS Microscopy How-to: Mount cleared samples in Lightsheet Z.1 (video)]] | ||
| * [[https://www.youtube.com/watch?v=vKHQudCKVGc|ZEISS Webinar: In Vivo and Cleared Tissue Imaging with Light Sheet Fluorescence Microscopy (video)]] | * [[https://www.youtube.com/watch?v=vKHQudCKVGc|ZEISS Webinar: In Vivo and Cleared Tissue Imaging with Light Sheet Fluorescence Microscopy (video)]] | ||
| + | --> | ||
| + | </html> | ||
| + | |||
| + | * [[https://hcbi.fas.harvard.edu/files/lightsheetz1_sample-preparation_zeiss.pdf| Sample Preparation for Light Sheet Microscopy (white paper)]] | ||
| + | * [[https://www.youtube.com/watch?v=Ki1wtoXIkis|Sample Preparation for Whole Organisms, Plants, and 3D Cell & Tissue Cultures (video)]] | ||
| + | |||
| ===== Tutorials for Image Analysis with Arivis ===== | ===== Tutorials for Image Analysis with Arivis ===== | ||
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| ==== LASERs ==== | ==== LASERs ==== | ||
| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | Solid State 445| 445 nm | 20 mW | | + | | Solid State 445 | 445 nm | 20 mW | |
| | Solid State 488 | 488 nm | 50 mW | | | Solid State 488 | 488 nm | 50 mW | | ||
| - | | Solid State 561 | 561 nm | 50 mW | | + | | Solid State 561 | 561 nm | 50 mW | |
| | Solid State 638 | 638 nm | 75 mW | | | Solid State 638 | 638 nm | 75 mW | | ||
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| * Turn off the system using the control units (PC, System and Incubation). | * Turn off the system using the control units (PC, System and Incubation). | ||
| - | * Remove the chamber, disassemble it, and rinse all the parts that compose it with distilled water. Leave them to dry in the appropriate box with the lid opened. __Warning__: If you have been trained and authorized by the staff to handle objectives, make sure to check and store the 2 illumination objectives, and to place the detection objective in the "washer" before cleaning it. You can ask the facility staff to inspect and clean them to prepare for the next user, if you're not comfortable with this procedure. | + | * Remove the chamber, disassemble it, and rinse all the parts that compose it with distilled water. Leave them to dry in the appropriate box with the lid opened. __Warning:__ If you have been trained and authorized by the staff to handle objectives, make sure to check and store the 2 illumination objectives, and to place the detection objective in the "washer" before cleaning it. You can ask the facility staff to inspect and clean them to prepare for the next user, if you're not comfortable with this procedure. |
| * Take your belongings (dishes, gloves, syringe, etc...), and leave the desk ready for the next user. | * Take your belongings (dishes, gloves, syringe, etc...), and leave the desk ready for the next user. | ||
oeiras_zeiss_lightsheet_z1.1733752493.txt.gz ยท Last modified: 2024/12/09 14:54 by bioimaging
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