oeiras_zeiss_lightsheet_z1
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision | ||
|
oeiras_zeiss_lightsheet_z1 [2024/12/06 18:24] bioimaging |
oeiras_zeiss_lightsheet_z1 [2025/03/14 11:01] (current) bioimaging |
||
|---|---|---|---|
| Line 1: | Line 1: | ||
| <html> | <html> | ||
| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=oeiras_zeiss_lightsheet_z.1_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=zeiss_lightsheet_z1_wiki_2024.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg"><img src="/facility/bioimaging/lib/exe/fetch.php?media=lightsheet.jpg" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a> Room 0B03 </a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 4533) <br> | + | <b>Location</b>: [Oeiras] Room 0B03<br> |
| <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | <b>Manufacturer</b>: <a href="http://www.zeiss.de/micro" target="_blank">ZEISS Microscopy</a><br> | ||
| <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | <b>Model</b>: <a href="https://www.youtube.com/watch?v=vKHQudCKVGc" target="_blank">Lightsheet Z.1</a> (click for Zeiss webinar)<br> | ||
| Line 18: | Line 18: | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="/facility/bioimaging/doku.php?id=zeiss_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> UPDATE Zeiss Lightsheet Z.1 Usage Statistics</a> | + | → <a href="/facility/bioimaging/doku.php?id=oeiras_lightsheet_z1_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> ZEISS Lightsheet Z.1 Usage Statistics</a> |
| </p> | </p> | ||
| </td> | </td> | ||
| Line 28: | Line 28: | ||
| {{ :lightsheet_z.jpg?0x380|}} The Zeiss Lightsheet Z.1 is a //light sheet fluorescence microscope// able to image optical sections of large samples at subcellular resolution and very fast rates, with almost no phototoxicity or bleaching. [[https://www.zeiss.com/content/dam/Microscopy/Products/imaging-systems/lattice-lightsheet/lattice-lightsheet-7/zeiss-lattice-lightsheet-7-beam-splitting.svg|It splits fluorescence excitation and detection into two separate light paths]], with the axis of illumination being perpendicular to the detection axis. | {{ :lightsheet_z.jpg?0x380|}} The Zeiss Lightsheet Z.1 is a //light sheet fluorescence microscope// able to image optical sections of large samples at subcellular resolution and very fast rates, with almost no phototoxicity or bleaching. [[https://www.zeiss.com/content/dam/Microscopy/Products/imaging-systems/lattice-lightsheet/lattice-lightsheet-7/zeiss-lattice-lightsheet-7-beam-splitting.svg|It splits fluorescence excitation and detection into two separate light paths]], with the axis of illumination being perpendicular to the detection axis. | ||
| - | [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=lightsheet_illumination.jpg|Since only a single thin section of the sample is illuminated by the light sheet]], optical sectioning is achieved without a pinhole or image processing deconvolution. Light from the in-focus plane is collected by a sCMOS camera rather than pixel by pixel as in a point scanning laser confocal. This allows you to collect images faster and with less excitation light than you would with many other optical-sectioning microscopy techniques. UPDATEThis system features two objectives with fields of view around 1 mm or 500 µm and resolutions between 0.15 µm and 0.3 µm, equipped with lasers spanning blue to far-red wavelengths (445 nm, 488 nm, 561 nm, and 638 nm) and appropriate fluorescence emission filters. Designed for live imaging, samples are embedded in agarose and preferably loaded into glass or plastic capillaries that provide heating and cooling to maintain physiological conditions, making the system ideal for observing live cells, embryos, Drosophila, and zebrafish. It is important to note that this Z.1 is optimized for live samples and does not include a kit for cleared samples; for such applications, other microscopes are recommended. Users should consult with facility staff to determine the best technique for their specific research needs. | + | [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=lightsheet_illumination.jpg|Since only a single thin section of the sample is illuminated by the light sheet]], optical sectioning is achieved without a pinhole or image processing deconvolution. Light from the in-focus plane is collected by a sCMOS camera rather than pixel by pixel as in a point scanning laser confocal. This allows you to collect images faster and with less excitation light than you would with many other optical-sectioning microscopy techniques. This system features two objectives with fields of view around 1 mm or 500 µm and resolutions between 0.15 µm and 0.3 µm, equipped with lasers spanning blue to far-red wavelengths (445 nm, 488 nm, 561 nm, and 638 nm) and appropriate fluorescence emission filters. Designed for live imaging, samples are embedded in agarose and preferably loaded into glass or plastic capillaries that provide heating and cooling to maintain physiological conditions, making the system ideal for observing live cells, embryos, Drosophila, and zebrafish. It is important to note that this Z.1 is optimized for live samples and does not include a kit for cleared samples; for such applications, other microscopes are recommended. Users should consult with facility staff to determine the best technique for their specific research needs. |
| {{:info.png?25|}} Click on the image on the right to see the system beam path in higher detail | {{:info.png?25|}} Click on the image on the right to see the system beam path in higher detail | ||
| Line 37: | Line 37: | ||
| * **Sample Size**: up to 1 x 1 x 2 cm | * **Sample Size**: up to 1 x 1 x 2 cm | ||
| - | * **Penetration depth**: up to 5.6 mm | ||
| + | <html> | ||
| + | <!-- | ||
| * [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=en_poster_overview-clearing-methods_rel-2.0.pdf|Clearing Methods in Microscopy (poster)]] | * [[https://imm.medicina.ulisboa.pt/facility/bioimaging/lib/exe/fetch.php?media=en_poster_overview-clearing-methods_rel-2.0.pdf|Clearing Methods in Microscopy (poster)]] | ||
| - | * [[https://hcbi.fas.harvard.edu/files/lightsheetz1_sample-preparation_zeiss.pdf| Sample Preparation for Light Sheet Microscopy (white paper)]] | ||
| * [[https://asset-downloads.zeiss.com/catalogs/download/mic/b91bc689-66ab-4d85-b3f6-c31ebea22ba7/EN_wp_Lightsheet-Z.1_Expanding-Clearing-Solutions.pdf| Improved Imaging of Cleared Samples (application note)]] | * [[https://asset-downloads.zeiss.com/catalogs/download/mic/b91bc689-66ab-4d85-b3f6-c31ebea22ba7/EN_wp_Lightsheet-Z.1_Expanding-Clearing-Solutions.pdf| Improved Imaging of Cleared Samples (application note)]] | ||
| - | * [[https://www.youtube.com/watch?v=Ki1wtoXIkis|Sample Preparation for Whole Organisms, Plants, and 3D Cell & Tissue Cultures (video)]] | ||
| * [[https://www.youtube.com/watch?v=Ql7T7LrvI_I|ZEISS Microscopy How-to: Mount cleared samples in Lightsheet Z.1 (video)]] | * [[https://www.youtube.com/watch?v=Ql7T7LrvI_I|ZEISS Microscopy How-to: Mount cleared samples in Lightsheet Z.1 (video)]] | ||
| * [[https://www.youtube.com/watch?v=vKHQudCKVGc|ZEISS Webinar: In Vivo and Cleared Tissue Imaging with Light Sheet Fluorescence Microscopy (video)]] | * [[https://www.youtube.com/watch?v=vKHQudCKVGc|ZEISS Webinar: In Vivo and Cleared Tissue Imaging with Light Sheet Fluorescence Microscopy (video)]] | ||
| + | --> | ||
| + | </html> | ||
| + | |||
| + | * [[https://hcbi.fas.harvard.edu/files/lightsheetz1_sample-preparation_zeiss.pdf| Sample Preparation for Light Sheet Microscopy (white paper)]] | ||
| + | * [[https://www.youtube.com/watch?v=Ki1wtoXIkis|Sample Preparation for Whole Organisms, Plants, and 3D Cell & Tissue Cultures (video)]] | ||
| + | |||
| ===== Tutorials for Image Analysis with Arivis ===== | ===== Tutorials for Image Analysis with Arivis ===== | ||
| Line 53: | Line 58: | ||
| ==== LASERs ==== | ==== LASERs ==== | ||
| ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ^ Laser Unit ^ Wavelength ^ Maximum Power ^ | ||
| - | | Solid State 445| 445 nm | 20 mW | | + | | Solid State 445 | 445 nm | 20 mW | |
| | Solid State 488 | 488 nm | 50 mW | | | Solid State 488 | 488 nm | 50 mW | | ||
| - | | Solid State 561 | 561 nm | 50 mW | | + | | Solid State 561 | 561 nm | 50 mW | |
| | Solid State 638 | 638 nm | 75 mW | | | Solid State 638 | 638 nm | 75 mW | | ||
| Line 108: | Line 113: | ||
| * Turn off the system using the control units (PC, System and Incubation). | * Turn off the system using the control units (PC, System and Incubation). | ||
| - | * Remove the chamber, disassemble it, and rinse all the parts that compose it with distilled water. Leave them to dry in the appropriate box with the lid opened. __Warning__: If you have been trained and authorized by the staff to handle objectives, make sure to check and store the 2 illumination objectives, and to place the detection objective in the "washer" before cleaning it. You can ask the facility staff to inspect and clean them to prepare for the next user, if you're not comfortable with this procedure. | + | * Remove the chamber, disassemble it, and rinse all the parts that compose it with distilled water. Leave them to dry in the appropriate box with the lid opened. __Warning:__ If you have been trained and authorized by the staff to handle objectives, make sure to check and store the 2 illumination objectives, and to place the detection objective in the "washer" before cleaning it. You can ask the facility staff to inspect and clean them to prepare for the next user, if you're not comfortable with this procedure. |
| * Take your belongings (dishes, gloves, syringe, etc...), and leave the desk ready for the next user. | * Take your belongings (dishes, gloves, syringe, etc...), and leave the desk ready for the next user. | ||
oeiras_zeiss_lightsheet_z1.1733505872.txt.gz · Last modified: 2024/12/06 18:24 by bioimaging
Page Tools
