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oeiras_zeiss_lightsheet_z1
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oeiras_zeiss_lightsheet_z1 [2026/02/17 17:35] bioimaging |
oeiras_zeiss_lightsheet_z1 [2026/02/17 17:46] (current) bioimaging [Filtersets] |
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| ==== Filtersets ==== | ==== Filtersets ==== | ||
| - | In each experiment you can only use Filter Turret A (installed by default) or B. Please let the facility staff know that you want to use filter turret B so that they can install and configure it in advance. | + | In each experiment you can only use Filter Turret A (installed by default) or B. Please let us know that you want to use filter turret B so that we can install and configure it in advance. |
| - | The list of fluorophores presented in both tables is meant to be representative. Use [[https://www.thermofisher.com/order/fluorescence-spectraviewer/|Spectra Viewer]] or [[https://www.fpbase.org|FPBase]] to determine which set of lasers and emission filters you want to optimize the system to the specific fluorophores you have in your sample. | + | === Filter Turret A (default) === |
| - | ^ Select Label ^ Camera #1 Filter ^ Camera #2 Filter ^ Fluorophores (Cam #1 : Cam #2) ^ Filter Turret ^ | + | ^ Select Label ^ Camera #1 Filter ^ Camera #2 Filter ^ Fluorophores (Cam #1 : Cam #2) ^ |
| - | | LP 510 | BP 460-500 | LP 585 | CFP, mCherry : RFP | A | | + | | LP 510 | <fc #00d5ff>460-500 nm</fc> | <fc #ffef00>> 585 nm</fc> | CFP, mCherry : RFP | |
| - | | LP 560 | BP 505-545 | BP 565-615 | GFP, mCherry : RFP | A | | + | | LP 560 | <fc #4aff00>505-545 nm</fc> | <fc #ffef00>565-615 nm</fc> | GFP, mCherry : RFP | |
| - | | LP 560 (2) | BP 505-545 | LP 585 | GFP, mCherry : RFP | A | | + | | LP 560 (2) | <fc #4aff00>505-545 nm</fc> | <fc #ffef00>> 585 nm</fc> | GFP, mCherry : RFP | |
| - | | LP 560 (3) | BP 505-545 | LP 660 | GFP, mPlum : mStrawberry, tdTomato | A | | + | | LP 560 (3) | <fc #4aff00>505-545 nm</fc> | <fc #ff0000>> 660 nm</fc> | GFP, mPlum : mStrawberry, tdTomato | |
| - | ^ Select Label ^ Camera #1 Filter ^ Camera #2 Filter ^ Fluorophores (Cam #1 : Cam #2) ^ Filter Turret ^ | + | === Filter Turret B === |
| - | | LP 510 | BP 460-500 | LP 660 | CFP, YFP : Cy5 | B | | + | |
| - | | LP 580 | BP 525-565 | LP 585 | YFP, mCherry : mPlum | B | | + | ^ Select Label ^ Camera #1 Filter ^ Camera #2 Filter ^ Fluorophores (Cam #1 : Cam #2) ^ |
| + | | LP 510 | <fc #00d5ff>460-500 nm</fc> | <fc #ff0000>> 660 nm</fc> | CFP, YFP : Cy5 | | ||
| + | | LP 580 | <fc #92ff00>525-565 nm</fc> | <fc #ffef00>> 585 nm</fc> | YFP, mCherry : mPlum | | ||
oeiras_zeiss_lightsheet_z1.txt · Last modified: 2026/02/17 17:46 by bioimaging
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