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--- oeiras_3i_marianas_sdc_bsl2 [2026/08/11 12:32]
bioimaging
+++ oeiras_3i_marianas_sdc_bsl2 [2026/09/08 01:19] (current)
bioimaging
@@ Line 28: / Line 28: @@
 ===== Microscope overview =====
-{{  :yokogawa_sd.png?350|}} The 3i Marianas SDC II super-resolution //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate point-like illumination covering the whole area “simultaneously”. Each small aperture serves as a detecting pinhole to remove out-of-focus light.
+{{  :yokogawa_sd.png?350|}} The 3i Marianas SDC (SoRa + TIRF) super-resolution //spinning disk confocal microscope// is a fast imaging system which provides a trade-off between confocality, resolution and speed, well suited for the observation of dynamics in living cells and small organisms. The Marianas SDC provides fast acquisition (up to ~100fps, with limited ROI, ~30FPS full frame), and with lower phototoxicity and bleaching. A spinning disk with multiple small pinholes is installed between the light source and specimen to generate point-like illumination covering the whole area “simultaneously”. Each small aperture serves as a detecting pinhole to remove out-of-focus light.
-This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 50um), DIC, TIRF, a removable heating stage with CO2 delivery, and a photomanipulation module (Phasor). The scanhead SoRa allows 1x, 2.8x and 4x magnification (for extra magnification and to allow Nyquist sampling and deconvolution).
+This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 50um), DIC, TIRF, a removable heating stage with CO2 delivery, and a photomanipulation module (Phasor). The Super-resolution by Optical Re-Assignment (SoRa) scanhead allows 1x, 2.8x and 4x magnification (for extra magnification and to allow Nyquist sampling and deconvolution). SoRa images have a 1.4x resolution improvement and deconvolved SoRa images have a 2x resolution improvement compared to standard spinning disk data.
+The system also allows for Total Internal Reflection Fluorescence (TIRF) imaging, which uses an evanescent wave with very low penetration depth, perfect for imaging live cell focal adhesions, membrane dynamics, and receptor function as well as in vitro experiments that require the thinnest optical section.
   * **Microscope**: [[https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti2-series/|Nikon Ti2 Eclipse]]
   * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-w1-confocal-scanner-unit/|Yokogawa CSU-W1]] + [[https://www.yokogawa.com/solutions/products-and-services/life-science/spinning-disk-confocal/uniformizer/#Overview|Uniformizer]]
   * **Camera**: [[https://www.teledynevisionsolutions.com/en-150/products/prime-95b/?segment=tvs&vertical=tvs%20-%20photometrics|Photometrics Prime 95B]]
+  * **Features**
+    * | Super-Resolution by Optical Re-Assignment (SoRa)
+    * | Phasor Holographic Photomanipulation
+    * | Vector2 TIRF
 {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

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