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oeiras_3i_marianas_sdc_bsl2 [2025/01/08 15:55]
bioimaging [Lasers]
oeiras_3i_marianas_sdc_bsl2 [2025/01/08 16:08] (current)
bioimaging [Objectives]
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 |  10  |  cBF-DIC ​ | - | any | - | |  10  |  cBF-DIC ​ | - | any | - |
  
-^  Position ​ ^  ​Filterset ​ ^  Reference ​ ​^ ​ Excitation ​ ^  Dichroic ​ ^  Emission ​ ^ +==== Nikon Ti2 filterset cubes ==== 
-      |  |  |  |  |+ 
 +^  ​Turret ​Position ​ ^  ​Filter cube  ​^ ​ Excitation ​ ^  Dichroic ​ ^  Emission ​ ^ 
 + 4 (lower deck)  ​ ​DAPI  ​378/52 | 409 | BP 447/60 |  
 +|  2 (lower deck)  ​| ​ ​GFP ​ | 472/30 | 495 | BP 520/35 |  
 + 3 (lower deck)  ​|  ​Texas Red  | 562/40 | 593 | BP 624/40 |  
 +|  6 (lower deck)  |  CSU (Confocal) ​ | - | empty | - 
  
  
 ==== Objectives ==== ==== Objectives ====
 ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^ ^  Magnification ​ ^  Model  ^  Immersion ​ ^  NA  ^  WD (mm)  ^  Reference ​ ^
-|   ​| ​            |   +|  10x  |  Plan Apochromat ​ |  Air  |  0.45  |  4  |[[https://​www.microscope.healthcare.nikon.com/​products/​optics/​selector/​comparison/​-179798 |MRD70170]] ​ | 
 +|  20x  |  Plan Fluor  |  Oil, W, G, Sil  |  0.75  |  0.51–0.33 ​ |  [[https://​www.microscope.healthcare.nikon.com/​products/​optics/​selector/​comparison/​-1827 |MRH07241]] ​ | 
 +|  40x  |  Plan Apochromat ​ |  Air  |  0.95  |  0.21  | [[https://​www.microscope.healthcare.nikon.com/​products/​optics/​selector/​comparison/​-179806 |MRD70470]] | 
 +|  60x  |  Plan-Apochromat ​ |  Water  |  1.20  |  0.31–0.28  ​|   [[https://​www.microscope.healthcare.nikon.com/​products/​optics/​selector/​comparison/​-1855|MRD07602]]  ​| 
 + ​60x  ​ Plan Apochromat ​ |  Oil  |  1.42  |  0.15  ​|   [[https://​www.microscope.healthcare.nikon.com/​products/​optics/​selector/​comparison/​-179808|MRD71670]] ​ | 
 +|  100x  |  Apochromat TIRF |  Oil  |  1.49  |  0.16–0.09 ​ |  [[https://​www.microscope.healthcare.nikon.com/​products/​optics/​selector/​comparison/​-1932|MRD01991]] ​ | 
  
 ==== Camera ==== ==== Camera ====
  
 +^  Model  ^  Frame Size  ^  Pixel Size (µm)  ^  Quantum Efficiency ​ ^
 +|  [[https://​www.teledynevisionsolutions.com/​products/​prime-95b/?​segment=tvs&​vertical=tvs%20-%20photometrics|Photometrics Prime 95B]]  |  1200 x 1200  |  11 x 11  |  > 95%  |
  
 ---- ----
 [[resources|Back to the Equipment page]] [[resources|Back to the Equipment page]]
oeiras_3i_marianas_sdc_bsl2.1736348132.txt.gz · Last modified: 2025/01/08 15:55 by bioimaging