oeiras_3i_marianas_sdc_bsl2
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oeiras_3i_marianas_sdc_bsl2 [2025/01/08 15:51] bioimaging [Microscope overview] |
oeiras_3i_marianas_sdc_bsl2 [2025/01/08 16:08] (current) bioimaging [Objectives] |
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| ===== System components ===== | ===== System components ===== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ==== Lasers ==== |
| - | | | | | | | | | + | ^ Laser line ^ Wavelength ^ Maximum Power ^ |
| + | | 405 | 405 nm | 100 mW | | ||
| + | | 445 | 445 nm | 150 mW | | ||
| + | | 488 | 488 nm | 150 mW | | ||
| + | | 561 | 561 nm | 100 mW | | ||
| + | | 594 | 594 nm | 100 mW | | ||
| + | | 637 | 637 nm | 140 mW | | ||
| + | |||
| + | ==== Emission Filters CSU W1 unit ==== | ||
| + | |||
| + | ^ Position ^ Filter name ^ Transmission (nm) ^ Dichroic ^ Reference ^ | ||
| + | | 1 | Quad (c###q) | 440/40 521/21 607/34 700/45 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=1201&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-440/521/607/700]] | | ||
| + | | 2 | c405s | 445/45 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=814&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-445/45]] | | ||
| + | | 3 | c488s | 525/30 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=902&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-525/30]] | | ||
| + | | 4 | c561s | 617/73 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=2874&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF02-617/73]] | | ||
| + | | 5 | c594s | 642/80 | 445/515/594 | [[https://www.fpbase.org/spectra/?s=716&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Chroma ET642/80]] | | ||
| + | | 6 | c637s | 692/40 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=1053&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-692/40]] | | ||
| + | | 7 | Dual (c###d) | 525 642 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=5890&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock EM01-R488/568]] | | ||
| + | | 8 | c445s | 482/25 | 445/515/594 | [[https://www.fpbase.org/spectra/?s=861&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-482/25]] | | ||
| + | | 9 | not config | 542/27 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=716&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-542/27]] | | ||
| + | | 10 | cBF-DIC | - | any | - | | ||
| + | |||
| + | ==== Nikon Ti2 filterset cubes ==== | ||
| + | |||
| + | ^ Turret Position ^ Filter cube ^ Excitation ^ Dichroic ^ Emission ^ | ||
| + | | 4 (lower deck) | DAPI | 378/52 | 409 | BP 447/60 | | ||
| + | | 2 (lower deck) | GFP | 472/30 | 495 | BP 520/35 | | ||
| + | | 3 (lower deck) | Texas Red | 562/40 | 593 | BP 624/40 | | ||
| + | | 6 (lower deck) | CSU (Confocal) | - | empty | - | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | | | | | | | + | | 10x | Plan Apochromat | Air | 0.45 | 4 |[[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179798 |MRD70170]] | |
| + | | 20x | Plan Fluor | Oil, W, G, Sil | 0.75 | 0.51–0.33 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1827 |MRH07241]] | | ||
| + | | 40x | Plan Apochromat | Air | 0.95 | 0.21 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806 |MRD70470]] | | ||
| + | | 60x | Plan-Apochromat | Water | 1.20 | 0.31–0.28 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1855|MRD07602]] | | ||
| + | | 60x | Plan Apochromat | Oil | 1.42 | 0.15 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179808|MRD71670]] | | ||
| + | | 100x | Apochromat TIRF | Oil | 1.49 | 0.16–0.09 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1932|MRD01991]] | | ||
| ==== Camera ==== | ==== Camera ==== | ||
| + | ^ Model ^ Frame Size ^ Pixel Size (µm) ^ Quantum Efficiency ^ | ||
| + | | [[https://www.teledynevisionsolutions.com/products/prime-95b/?segment=tvs&vertical=tvs%20-%20photometrics|Photometrics Prime 95B]] | 1200 x 1200 | 11 x 11 | > 95% | | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
oeiras_3i_marianas_sdc_bsl2.1736347885.txt.gz · Last modified: 2025/01/08 15:51 by bioimaging
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