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oeiras_3i_marianas_sdc_bsl2
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oeiras_3i_marianas_sdc_bsl2 [2026/01/18 16:06] bioimaging [Lasers] |
oeiras_3i_marianas_sdc_bsl2 [2026/01/18 16:19] (current) bioimaging |
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| | Orange | <fc #ffd200>594 nm</fc> | 100 mW | | | Orange | <fc #ffd200>594 nm</fc> | 100 mW | | ||
| | Red | <fc #ff3000>637 nm</fc> | 140 mW | | | Red | <fc #ff3000>637 nm</fc> | 140 mW | | ||
| - | ==== Emission Filters CSU W1 unit ==== | ||
| - | ^ Position ^ Filter name ^ Transmission (nm) ^ Dichroic ^ Reference ^ | + | ==== Filtersets (eyepieces) ==== |
| - | | 1 | Quad (c###q) | 440/40 521/21 607/34 700/45 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=1201&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-440/521/607/700]] | | + | |
| - | | 2 | c405s | 445/45 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=814&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-445/45]] | | + | |
| - | | 3 | c488s | 525/30 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=902&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-525/30]] | | + | |
| - | | 4 | c561s | 617/73 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=2874&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF02-617/73]] | | + | |
| - | | 5 | c594s | 642/80 | 445/515/594 | [[https://www.fpbase.org/spectra/?s=716&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Chroma ET642/80]] | | + | |
| - | | 6 | c637s | 692/40 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=1053&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-692/40]] | | + | |
| - | | 7 | Dual (c###d) | 525 642 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=5890&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock EM01-R488/568]] | | + | |
| - | | 8 | c445s | 482/25 | 445/515/594 | [[https://www.fpbase.org/spectra/?s=861&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-482/25]] | | + | |
| - | | 9 | not config | 542/27 | 405/488/561/640 | [[https://www.fpbase.org/spectra/?s=716&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-542/27]] | | + | |
| - | | 10 | cBF-DIC | - | any | - | | + | |
| - | + | ||
| - | ==== Nikon Ti2 filterset cubes ==== | + | |
| - | + | ||
| - | ^ Turret Position ^ Filter cube ^ Excitation ^ Dichroic ^ Emission ^ | + | |
| - | | 4 (lower deck) | DAPI | 378/52 | 409 | BP 447/60 | | + | |
| - | | 2 (lower deck) | GFP | 472/30 | 495 | BP 520/35 | | + | |
| - | | 3 (lower deck) | Texas Red | 562/40 | 593 | BP 624/40 | | + | |
| - | | 6 (lower deck) | CSU (Confocal) | - | empty | - | | + | |
| + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ | ||
| + | | 4 (lower deck) | Blue | 352-404 nm | 409 | <fc #0035ff>417-477 nm</fc> | | ||
| + | | 2 (lower deck) | Green | 457-487 nm | 495 nm | <fc #36ff00>503-538 nm</fc> | | ||
| + | | 3 (lower deck) | Orange | 542-582 nm | 593 nm | <fc #ff6700>604-644 nm</fc> | | ||
| + | | 6 (lower deck) | CSU (Confocal) | - | - | - | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| + | |||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 10x | Plan Apochromat | Air | 0.45 | 4 |[[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179798 |MRD70170]] | | + | | <fc #fef042>10x</fc> | Plan Apochromat | Air | 0.45 | 4 |[[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179798 |MRD70170]] | |
| - | | 20x | Plan Fluor | Oil, W, G, Sil | 0.75 | 0.51–0.33 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1827 |MRH07241]] | | + | | <fc #0aa05c>20x</fc> | Plan Fluor | Oil, W, G, Sil | 0.75 | 0.51–0.33 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1827 |MRH07241]] | |
| - | | 40x | Plan Apochromat | Air | 0.95 | 0.21 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806 |MRD70470]] | | + | | <fc #01b3f5>40x</fc> | Plan Apochromat | Air | 0.95 | 0.21 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806 |MRD70470]] | |
| - | | 60x | Plan-Apochromat | Water | 1.20 | 0.31–0.28 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1855|MRD07602]] | | + | | <fc #2c80c0>60x</fc> | Plan-Apochromat | Water | 1.20 | 0.31–0.28 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1855|MRD07602]] | |
| - | | 60x | Plan Apochromat | Oil | 1.42 | 0.15 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179808|MRD71670]] | | + | | <fc #2c80c0>60x</fc> | Plan-Apochromat | Oil | 1.42 | 0.15 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179808|MRD71670]] | |
| | 100x | Apochromat TIRF | Oil | 1.49 | 0.16–0.09 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1932|MRD01991]] | | | 100x | Apochromat TIRF | Oil | 1.49 | 0.16–0.09 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-1932|MRD01991]] | | ||
| + | |||
| + | ==== Emission Filters CSU W1 ==== | ||
| + | |||
| + | ^ Position ^ Filter name ^ Dichroic ^ Transmission ^ Reference ^ | ||
| + | | 1 | QUAD (c###q) | 405/488/561/640 | <fc #0000ff>420-460 nm</fc> | [[https://www.fpbase.org/spectra/?s=1201&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-440/521/607/700]] | | ||
| + | | ::: | ::: | ::: | <fc #3aff00>511-532 nm</fc> | ::: | | ||
| + | | ::: | ::: | ::: | <fc #ffa600>590-624 nm</fc> | ::: | | ||
| + | | ::: | ::: | ::: | <fc #ff0000>678-723 nm</fc> | ::: | | ||
| + | | 2 | c405s | 405/488/561/640 | <fc #0028ff>423-468 nm</fc> | [[https://www.fpbase.org/spectra/?s=814&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-445/45]] | | ||
| + | | 3 | c488s | 405/488/561/640 | <fc #4aff00>510-540 nm</fc> | [[https://www.fpbase.org/spectra/?s=902&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-525/30]] | | ||
| + | | 4 | c561s | 405/488/561/640 | <fc #ff8200>581-654 nm</fc> | [[https://www.fpbase.org/spectra/?s=2874&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF02-617/73]] | | ||
| + | | 5 | c594s | 445/515/594 | <fc #ff2100>602-682 nm</fc> | [[https://www.fpbase.org/spectra/?s=716&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Chroma ET642/80]] | | ||
| + | | 6 | c637s | 405/488/561/640 | <fc #ff0000>672-712 nm</fc> | [[https://www.fpbase.org/spectra/?s=1053&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-692/40]] | | ||
| + | | 7 | DUAL (c###d) | 405/488/561/640 | <fc #4aff00>510-540 nm</fc> | [[https://www.fpbase.org/spectra/?s=5890&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock EM01-R488/568]] | | ||
| + | | ::: | ::: | ::: | <fc #ff2100>602-682 nm</fc> | ::: | | ||
| + | | 8 | c445s | 445/515/594 | <fc #00deff>470-495 nm</fc> | [[https://www.fpbase.org/spectra/?s=861&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-482/25]] | | ||
| + | | 9 | c488/YFP | 405/488/561/640 | <fc #88ff00>529-556 nm</fc> | [[https://www.fpbase.org/spectra/?s=716&showY=0&showX=1&showGrid=0&areaFill=1&logScale=0&scaleEC=0&scaleQY=0&shareTooltip=1&palette=wavelength|Semrock FF01-542/27]] | | ||
| + | | 10 | cBF-DIC | - | any | - | | ||
| ==== Camera ==== | ==== Camera ==== | ||
oeiras_3i_marianas_sdc_bsl2.txt · Last modified: 2026/01/18 16:19 by bioimaging
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