nikon_eclipse_ti
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nikon_eclipse_ti [2023/02/22 02:04] bioimaging [Filtersets Turret 2 (bottom)] |
nikon_eclipse_ti [2025/12/12 01:27] (current) bioimaging [Objectives] |
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| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_nikon_eclipse_ti_name_GIMM_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2022.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2024.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new_p2a24.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new_p2a24.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br> |
| <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br> | <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br> | ||
| <b>Model</b>: Eclipse Ti<br> | <b>Model</b>: Eclipse Ti<br> | ||
| <b>Nickname</b>: "006"<br> | <b>Nickname</b>: "006"<br> | ||
| - | <b>Software</b>: NIS-Elements AR 5.11.01<br> | + | <b>Software</b>: NIS-Elements AR 5.42.05<br> |
| <b>Year</b>: 2010 <br> | <b>Year</b>: 2010 <br> | ||
| <b>SN</b>: 602805 <br> | <b>SN</b>: 602805 <br> | ||
|   <br> |   <br> | ||
| - |   <br> | + | Data will be deleted after: <b>3 months</b> |
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| Line 27: | Line 27: | ||
| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | {{ :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Andor Neo 5.5 monochrome cooled sCMOS camera ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]]. | + | |
| + | The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome cooled sCMOS camera. | ||
| * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]] | ||
| * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] | * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]] | ||
| - | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== System components ===== | ===== System components ===== | ||
| - | ==== Filtersets Turret 1 (top) ==== | + | ==== Filtersets ==== |
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ^ Position ^ Filterset ^ Excitation ^ Dichroic ^ Emission ^ Reference ^ |
| | 1 | Empty ||||| | | 1 | Empty ||||| | ||
| - | | 2 | DAPI | MBE41300 | 340-380 nm | 400 nm | 435-485 nm | | + | | 2 | Blue | 340-380 nm | 400 nm | <fc #007bff>435-485 nm</fc> | MBE41300 | |
| - | | 3 | FITC | MBE44720 | 465-495 nm | 505 nm | 515-555 nm | | + | | 3 | Green | 465-495 nm | 505 nm | <fc #70ff00>515-555 nm</fc> | MBE44720 | |
| - | | 4 | TRITC | MBE45600 | 528-553 nm | 565 nm | 590-650 nm | | + | | 4 | Orange | 528-553 nm | 565 nm | <fc #ff7700>590-650 nm</fc> | MBE45600 | |
| - | | 5 | Cy5 | 2SK4CY50 | 608-648 nm | 660 nm | 672-712 nm | | + | | 5 | Red | 608-648 nm | 660 nm | <fc #ff0000>672-712 nm</fc> | 2SK4CY50 | |
| | 6 | Empty ||||| | | 6 | Empty ||||| | ||
| - | |||
| - | ==== Filtersets Turret 2 (bottom) ==== | ||
| - | |||
| - | ^ Position ^ Filterset ^ | ||
| - | | 1 | Empty | | ||
| - | | 2 | Empty | | ||
| - | | 3 | Empty | | ||
| - | | 4 | Empty | | ||
| - | | 5 | Empty | | ||
| - | | 6 | Fura-2 | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | 10x | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | | + | | <fc #fef042>10x</fc> | CFI Plan Fluor DLL Ph1 | Air | 0.30 | 16.00 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1835|MRH10101]] | |
| - | | 20x | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]] | | + | | <fc #0aa05c>20x</fc> | CFI S Plan Fluor ELWD ADM Ph1 | Air | 0.45 | 6.90 - 8.20 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1813|MRH48230]] | |
| - | | 40x | CFI CFI Plan Apochromat Lambda D | Air | 0.95 | 0.21 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806|MRH70470]] | | + | | <fc #01b3f5>40x</fc> | CFI CFI Plan Apochromat Lambda D | Air | 0.95 | 0.21 | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179806|MRH70470]] | |
| - | | 40x | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | | + | | <fc #01b3f5>40x</fc> | CFI S Plan Fluor ELWD ADM Ph2 | Air | 0.60 | 2.80 - 3.60 | [[https://www.microscope.healthcare.nikon.com/en_EU/selectors/objective-comparison/-1814|MRH48430]] | |
| - | | 60x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | | + | | <fc #2c80c0>60x</fc> | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | |
| | 100x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | | | 100x | Plan Apo DM Ph3 | Oil | 1.40 | 0.13 | | | ||
nikon_eclipse_ti.1677027893.txt.gz · Last modified: 2023/02/22 02:04 by bioimaging
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