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--- nikon_eclipse_ti [2023/02/22 02:04]
bioimaging [Filtersets Turret 2 (bottom)]
+++ nikon_eclipse_ti [2025/01/14 16:33] (current)
bioimaging [Microscope overview]
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-<img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_name_new.png">
+<img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_nikon_eclipse_ti_name_GIMM_2024.png">
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-<a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2022.png" width=300></a>
+<a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_wiki_2024.png" width=300></a>
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-<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new_p2a24.png">Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br>
+<b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=nikon_eclipse_ti_map_new_p2a24.png">[Lisbon] Room P2-A-24</a> (<img src="/facility/bioimaging/lib/exe/fetch.php?media=phone_neg.png" width=15> 47219) <br>
 <b>Manufacturer</b>: <a href="https://www.microscope.healthcare.nikon.com/en_EU/" target="_blank">Nikon Instruments</a><br>
 <b>Model</b>: Eclipse Ti<br>
 <b>Nickname</b>: "006"<br>
-<b>Software</b>: NIS-Elements AR 5.11.01<br>
+<b>Software</b>: NIS-Elements AR 5.42.05<br>
 <b>Year</b>: 2010 <br>
 <b>SN</b>: 602805 <br>
 &nbsp <br>
-&nbsp <br>
+Data will be deleted after: <b>3 months</b>
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 &nbsp <br>
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 ===== Microscope overview =====
-{{  :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using either Metamorph or Nikon Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with a Andor Neo 5.5 monochrome cooled sCMOS camera ideal for time-lapse and tile-and-stitch microscopy. You can perform Z-stack acquisition and use [[https://huygens.i3s.up.pt/login.php|PPBI Huygens RM]] [[http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconvolutionhome.html|deconvolution]] server to significantly improve image resolution even in thick samples. If don't want to use deconvolution, check out one of the confocal systems such as the point scanners [[zeiss_lsm_710|Zeiss LSM 710]] and [[zeiss_lsm_880|Zeiss LSM 880]] or spinning disks [[3i_marianas_sdc|3i Marianas SDC]] and [[zeiss_cell_observer_sd|Zeiss Cell Observer SD]]. If your personal computer cannot handle all the data you collected, check out the [[colossus|Colossus]].
+{{  :widefield_lightpath.png?0x300}}The Nikon Eclipse Ti is a fully motorized inverted //widefield fluorescence microscope// ideal for live-cell imaging applications. It is equipped with a small stage incubator for temperature control and CO2 supply, and with Perfect Focus System for hardware focus control during long time-lapse acquisitions. Using NIS-Elements software you can control the motorized filters, shutters and stage to simultaneously set up multi-color time-lapse imaging experiments in multiple stage positions. It is equipped with an Andor Neo 5.5 monochrome cooled sCMOS camera.
   * **Microscope**: [[https://www.microscope.healthcare.nikon.com/en_EU/products/inverted-microscopes/eclipse-ti-series|Nikon Eclipse Ti]]
   * **Camera**: [[https://andor.oxinst.com/products/scmos-camera-series/neo-5-5-scmos|Andor Neo 5.5 sCMOS]]
-{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link.
+This microscope is also equipped with a [[https://www.ionoptix.com/products/systems/calcium-and-contractility-system/|Ionoptix Calcium and Contractility system]] for high-speed simultaneous acquisition of Fura-2 fluorescence with video-based cell length measurement to study myocyte function, contracting spontaneously or with field stimulation.
+{{::ionoptix_realtime-acquisition-cell-length-600x500.jpg?200  |}}
+  * **High-speed contractility camera**: [[https://www.ionoptix.com/products/components/sensors/myocam-s3/|MyoCam-S3]]
+  * **Electrical Field Stimulator**: [[https://www.ionoptix.com/products/components/stimulators/myopacer-cell-stimulator/|MyoPacer]]
+  * **LED Light Source**: [[https://www.ionoptix.com/products/components/light-sources/led-light-source/|UV LEDs for Fura-2 excitation]]
+  * **High-speed PMT**: [[https://www.ionoptix.com/products/components/sensors/pmt-sub-system/|PMT400]] for photon counting at 1kHz
+  * **Software**: [[https://www.ionoptix.com/products/software/ionwizard-core-and-analysis/|IonWizard]] 7.7.3.180
+{{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.
 ===== System components =====

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