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microscopytools [2020/01/02 00:16] bioimaging [Image analysis and visualization software] |
microscopytools [2025/01/14 16:41] (current) bioimaging |
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- | <html><img src="/facility/bioimaging/lib/exe/fetch.php?media=software_neg.png"><BR></html> | + | <html><img src="/facility/bioimaging/lib/exe/fetch.php?media=image_analysis_section.png"><BR></html> |
- | ===== Software developed by the Bioimaging Facility ===== | + | ===== Software developed by the Bioimaging Platform ===== |
- | * {{:icon_ubi.png?30|}} **[[software_cclab|Cell Counter L*a*b*]]** | + | * {{:icon_staqtool.png?30|}} **[[software_staqtool|STaQTool - Spot Tracking and Quantification Tool]]** |
+ | * Track diffraction-limited spots in 2D or 3D time-lapse sequences | ||
+ | * Quantify total fluorescence intensity with Gaussian fitting | ||
+ | * Detect single events of fluorescence gain and loss | ||
+ | |||
+ | * {{:logo.png?30|}} **[[software_cclab|Cell Counter L*a*b*]]** | ||
* Count cells with specific nuclear and cytoplasmic staining in color images (immunohistochemical staining) | * Count cells with specific nuclear and cytoplasmic staining in color images (immunohistochemical staining) | ||
- | * {{:icon_ubi.png?30|}} **[[software_mcclsm|Multichannel Cell Counter LSM]]** | + | * {{:logo.png?30|}} **[[software_cclab2|Cell Counter L*a*b* 2]]** |
+ | * Count 2 different cell populations with blue and brown staining in color images (immunohistochemical staining) | ||
+ | |||
+ | * {{:logo.png?30|}} **[[software_mccczi|Multichannel Cell Counter CZI]]** | ||
+ | * Count cells with specific fluorescent labeling combinations - CZI files | ||
+ | |||
+ | * {{:logo.png?30|}} **[[software_mcclsm|Multichannel Cell Counter LSM]]** | ||
* Count cells with specific fluorescent labeling combinations - LSM files | * Count cells with specific fluorescent labeling combinations - LSM files | ||
- | * {{:icon_ubi.png?30|}} **[[software_mccrgb|Multichannel Cell Counter RGB]]** | + | * {{:logo.png?30|}} **[[software_mccrgb|Multichannel Cell Counter RGB]]** |
* Count cells with specific fluorescent labeling combinations - RGB TIF files | * Count cells with specific fluorescent labeling combinations - RGB TIF files | ||
- | * {{:icon_ubi.png?30|}} **[[software_mcc4tiff|Multichannel Cell Counter 4TIFF]]** | + | * {{:logo.png?30|}} **[[software_mcc4tiff|Multichannel Cell Counter 4TIFF]]** |
* Count cells with specific fluorescent labeling combinations - 4 TIF files | * Count cells with specific fluorescent labeling combinations - 4 TIF files | ||
- | |||
- | * {{:icon_staqtool.png?30|}} **[[software_staqtool|STaQTool - Spot Tracking and Quantification Tool]]** | ||
- | * Track diffraction-limited spots in 2D or 3D time-lapse sequences | ||
- | * Quantify total fluorescence intensity with Gaussian fitting | ||
- | * Detect single events of fluorescence gain and loss | ||
- | |||
===== Image analysis and visualization software ===== | ===== Image analysis and visualization software ===== | ||
- | * {{:imagej2-icon.png?40|}} **[[http://rsbweb.nih.gov/ij/|ImageJ]]** | + | * {{:imagej_icon.png?35|}} **[[http://rsbweb.nih.gov/ij/|ImageJ]]** |
* The Swiss army knife of microscopy image processing | * The Swiss army knife of microscopy image processing | ||
* {{:fiji-icon.png?35|}} **[[http://fiji.sc/Fiji|FIJI]]** | * {{:fiji-icon.png?35|}} **[[http://fiji.sc/Fiji|FIJI]]** | ||
- | * Fiji Is Just ImageJ (and a lot of plugins organized into a coherent menu structure) | + | * Fiji Is Just ImageJ focused on the visualization and analysis of microscope images in 2D, 3D, 4D and 5D |
* {{icy-icon.png?35|}} **[[http://icy.bioimageanalysis.org/|ICY]]** | * {{icy-icon.png?35|}} **[[http://icy.bioimageanalysis.org/|ICY]]** | ||
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===== Image viewers from microscopy companies ===== | ===== Image viewers from microscopy companies ===== | ||
- | * {{zeiss_zen.jpg?35|}} **[[https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html|Zeiss ZEN lite for Windows]]** | + | * {{zen_icon.png?35|}} **[[https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html|Zeiss ZEN lite for Windows]]** |
* Supports CZI file format | * Supports CZI file format | ||
- | * {{slidebook-icon.png?35|}} **[[https://www.intelligent-imaging.com/slidebook#reader|3i Slidebook Reader]]** | + | * {{slidebook_icon.png?35|}} **[[https://www.intelligent-imaging.com/slidebook#reader|3i Slidebook Reader]]** |
* Supports SLD file format | * Supports SLD file format | ||
+ | |||
+ | * {{::las_x.png?35|}} **[[https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/|LAS X Core]]** | ||
+ | * Supports LIF file format | ||
+ | |||
+ | * {{::imaris_logo.png?35|}} **[[https://imaris.oxinst.com/imaris-viewer|Imaris Viewer]]** | ||
+ | * For viewing raw images as well as those analyzed within Imaris | ||
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[[start|Back to the Bioimaging Wiki]] | [[start|Back to the Bioimaging Wiki]] |