This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision | ||
|
leica_dm2500 [2022/08/31 23:55] bioimaging |
leica_dm2500 [2026/02/17 17:49] (current) bioimaging [Microscope overview] |
||
|---|---|---|---|
| Line 1: | Line 1: | ||
| <html> | <html> | ||
| - | <img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_name_new.png"> | + | <img src="/facility/bioimaging/lib/exe/fetch.php?media=lisbon_leica_dm2500_name_gimm_2024.png"> |
| <table style="border:0px solid white;"> | <table style="border:0px solid white;"> | ||
| <tr style="border:0px solid white;"> | <tr style="border:0px solid white;"> | ||
| <td style="border:0px solid white;"> | <td style="border:0px solid white;"> | ||
| - | <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_wiki_2022.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_wiki_2022.png" width=300></a> | + | <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_wiki_2024.png"><img src="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_wiki_2024.png" width=300></a> |
| <td style="border:0px solid white;"> <p style="line-height:1.8"> | <td style="border:0px solid white;"> <p style="line-height:1.8"> | ||
| - | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_map_new.png">Room P2-A-15</a><br> | + | <b>Location</b>: <a href="/facility/bioimaging/lib/exe/fetch.php?media=leica_dm2500_map_new.png">[Lisbon] Room P3-A-16b</a><br> |
| <b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br> | <b>Manufacturer</b>: <a href="http://www.leica-microsystems.com/home/" target="_blank">Leica Microsystems</a><br> | ||
| <b>Model</b>: DM2500<br> | <b>Model</b>: DM2500<br> | ||
| Line 14: | Line 14: | ||
| <b>SN</b>: 285533-102006<br> | <b>SN</b>: 285533-102006<br> | ||
|   <br> |   <br> | ||
| + | Data will be deleted after: <b>3 months</b> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
|   <br> |   <br> | ||
| - | → <a href="https://my.agendo.science/calendar/?id=MzNjYWxlbmRhcnM="><img src="/facility/bioimaging/lib/exe/fetch.php?media=date.png"> Leica DM2500 Booking</a> | ||
| - | <br> | ||
| → <a href="/facility/bioimaging/doku.php?id=leica_dm2500_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM2500 Usage Statistics</a> | → <a href="/facility/bioimaging/doku.php?id=leica_dm2500_usage"><img src="/facility/bioimaging/lib/exe/fetch.php?media=chart_line.png"> Leica DM2500 Usage Statistics</a> | ||
| </p> | </p> | ||
| Line 27: | Line 26: | ||
| ===== Microscope overview ===== | ===== Microscope overview ===== | ||
| - | The Leica DM2500 is an upright //brightfield microscope// equipped with a color camera (Leica DFC450) for brightfield and differential interference contrast (DIC) imaging. If you need to perform fluorescence imaging try the [[zeiss_axiovert_200m|Zeiss Axiovert 200M]] instead. | + | The Leica DM2500 is an upright //brightfield microscope// equipped with a color camera (Leica DFC450) for brightfield and differential interference contrast (DIC) imaging. |
| - | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the iMM server using the desktop link. | + | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. |
| ===== System components ===== | ===== System components ===== | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size (μm) ^ 10 μm = ^ Reference ^ | ^ Magnification ^ Model ^ Type ^ NA ^ WD (mm) ^ pixel size (μm) ^ 10 μm = ^ Reference ^ | ||
| - | | 1.25x | HCX PL FLUOTAR | Dry | 0.04 | 3.70 | 4.90 | 2.0 pixels | 506215 | | + | | <fc #88888a>1.25x</fc> | HCX PL FLUOTAR | Dry | 0.04 | 3.70 | 4.90 | 2.0 pixels | 506215 | |
| - | | 5x | N PLAN | Dry | 0.12 | 14.00 | 1.23 | 8.1 pixels | 506302 | | + | | <fc #df2b37>5x</fc> | N PLAN | Dry | 0.12 | 14.00 | 1.23 | 8.1 pixels | 506302 | |
| - | | 10x | HC PL FLUOTAR | Dry | 0.30 | 11.00 | 0.61 | 16.2 pixels | 506507 | | + | | <fc #fef042>10x</fc> | HC PL FLUOTAR | Dry | 0.30 | 11.00 | 0.61 | 16.2 pixels | 506507 | |
| - | | 20x | HC PL FLUOTAR | Dry | 0.50 | 1.15 | 0.30 | 32.5 pixels | 506506 | | + | | <fc #0aa05c>20x</fc> | HC PL FLUOTAR | Dry | 0.50 | 1.15 | 0.30 | 32.5 pixels | 506506 | |
| - | | 40x | HCX PL FLUOTAR | Dry | 0.75 | 0.40 | 0.15 | 65 pixels | 506144 | | + | | <fc #01b3f5>40x</fc> | HCX PL FLUOTAR | Dry | 0.75 | 0.40 | 0.15 | 65 pixels | 506144 | |
| | 100x | HCX PL FLUOTAR | Oil | 1.30 | 0.22 | 0.06 | 162.5 pixels | 506195 | | | 100x | HCX PL FLUOTAR | Oil | 1.30 | 0.22 | 0.06 | 162.5 pixels | 506195 | | ||
| Line 44: | Line 43: | ||
| ==== Camera ==== | ==== Camera ==== | ||
| ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ^ Model ^ Type ^ Frame Size ^ Pixel Size (µm) ^ | ||
| - | | [[https://www.leica-microsystems.com/products/microscope-cameras/p/leica-dfc450-c/|Leica DFC450]] | color CCD | 2560 × 1920 | 3.4 x 3.4 | | + | | Leica DFC450 | color CCD | 2560 × 1920 | 3.4 x 3.4 | |
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||