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ivis_lumina_lt
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ivis_lumina_lt [2026/02/05 10:59] bioimaging [System Specifications] |
ivis_lumina_lt [2026/09/17 14:11] (current) bioimaging [System overview] |
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| {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | {{:warning.png?25|}} Data files older than **3 months** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | ||
| + | Additionally, IVIS Lumina LT is equipped with a [[https://www.kentscientific.com/products/somnoflo/?srsltid=AfmBOoo0Ou5Ldx5Qcyl9O-LhECWrG5Y_qDed5DOdSe9kc8OGR6y0p3su|SomnoFlo® anaesthesia system]] for experiments with live animals using isoflurane. This system allows the use of smaller amounts of anaesthesia when carrying out live-imaging experiments, keeps mice stable during image acquisition, and reduces their recovery time, preventing the loss of animals. The setup is currently able to keep **up to three mice receiving vaporized isoflurane inside the IVIS Lumina LT**, while being imaged at the same time. | ||
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| + | Users that are planning on using the anaesthesia system should contact the **Rodent Platform's designated veterinarian, Daniel Costa** (daniel.costa@gimm.pt), to schedule a training session. | ||
| ===== System booking rules ===== | ===== System booking rules ===== | ||
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| * **Camera sensor**: Back-thinned, back-illuminated CCD | * **Camera sensor**: Back-thinned, back-illuminated CCD | ||
| * **CCD cooling temperature**: -90 °C | * **CCD cooling temperature**: -90 °C | ||
| - | * **Frame size**: 1024 x 1024 (default binning mode is 4x4 so acquired image is 256x256) - TO BE CONFIRMED! | + | * **Frame size**: 1024 x 1024 (default binning mode is 4x4 so acquired image is 256x256) <= CONFIRM WITH STAFF! |
| * **Pixel size**: 13 μm (52 in small binning mode) | * **Pixel size**: 13 μm (52 in small binning mode) | ||
| * **Quantum Efficiency**: >85% Efficiency 500-700 nm, >55% Efficiency 400-500 nm, >35% Efficiency 700-900 nm | * **Quantum Efficiency**: >85% Efficiency 500-700 nm, >55% Efficiency 400-500 nm, >35% Efficiency 700-900 nm | ||
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| * **Lens**: f/.95 – f/16, 50 mm | * **Lens**: f/.95 – f/16, 50 mm | ||
| * **Sensitivity**: 100 photons/s/cm²/sr | * **Sensitivity**: 100 photons/s/cm²/sr | ||
| - | * **Read noise**: < 3 electrons for bin = 1, 2, 4; < 5 electrons for bin = 8, 16 <= CONFIRM THESE MODES! | + | * **Read noise**: < 3 electrons for bin = 1, 2, 4; < 5 electrons for bin = 8, 16 <= CONFIRM WITH STAFF! |
| * **Dark Current**: < 120 electrons/s/cm2; or 2 x 10-4 electrons/s/pixel (typical) | * **Dark Current**: < 120 electrons/s/cm2; or 2 x 10-4 electrons/s/pixel (typical) | ||
| * **Excitation fluorescence filters**: 10 | * **Excitation fluorescence filters**: 10 | ||
| * **Emmission fluorescence filters**: 4 | * **Emmission fluorescence filters**: 4 | ||
| + | * **Linear regime of camera is between 600 - 60 000 counts** (avoid operating above 60k!) | ||
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| ==== Excitation and Emission Filtersets ==== | ==== Excitation and Emission Filtersets ==== | ||
ivis_lumina_lt.1770285563.txt.gz · Last modified: 2026/02/05 10:59 by bioimaging
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