Bioimaging Wiki

The IVIS Lumina LT fluorescence/bioluminescence imaging system is equipped with 2D bioluminescence, fluorescence, and radioisoptic (Cerenkov) imaging capabilities, providing high sensitivity, ease of use, and flexibility to image fluorescent and/or bioluminescent reporters both in vivo and in vitro. The system includes a highly sensitive CCD camera, a light-tight imaging chamber with complete computer automation, and the Living Image software package for image acquisition and analysis.

Features:

• 2D Bioluminescence
• 2D Fluorescence
• Radioisotopic Cerenkov Imaging
• Imaging of up to 5 mice
• Compute Pure Spectrum Spectral Unmixing
• Extended NIR Range
• Absolute Calibration to NIST® Standards

Data files older than 3 months will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link.

You can book the system with 30 days in advance.

• Camera sensor: Back-thinned, back-illuminated CCD
• CCD cooling temperature: -90 °C
• Frame size: 1024 x 1024
• Pixel size: 13 μm
• Quantum Efficiency: >85% Efficiency 500-700 nm, >55% Efficiency 400-500 nm, >35% Efficiency 700-900 nm
• Minimum FOV: 5 x 5 cm
• Lens: f/.95 – f/16, 50 mm
• Sensitivity: 100 photons/s/cm²/sr
• Read noise: < 3 electrons for bin = 1, 2, 4; < 5 electrons for bin = 8, 16
• Dark Current: < 120 electrons/s/cm2; or 2 x 10-4 electrons/s/pixel (typical)
• Excitation fluorescence filters: 10
• Emmission fluorescence filters: 4

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