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ivis_lumina_lt [2025/11/21 19:06] bioimaging |
ivis_lumina_lt [2026/02/05 11:29] (current) bioimaging [System Specifications] |
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| * **Camera sensor**: Back-thinned, back-illuminated CCD | * **Camera sensor**: Back-thinned, back-illuminated CCD | ||
| * **CCD cooling temperature**: -90 °C | * **CCD cooling temperature**: -90 °C | ||
| - | * **Frame size**: 1024 x 1024 | + | * **Frame size**: 1024 x 1024 (default binning mode is 4x4 so acquired image is 256x256) <= CONFIRM WITH STAFF! |
| - | * **Pixel size**: 13 μm | + | * **Pixel size**: 13 μm (52 in small binning mode) |
| * **Quantum Efficiency**: >85% Efficiency 500-700 nm, >55% Efficiency 400-500 nm, >35% Efficiency 700-900 nm | * **Quantum Efficiency**: >85% Efficiency 500-700 nm, >55% Efficiency 400-500 nm, >35% Efficiency 700-900 nm | ||
| * **Minimum FOV**: 5 x 5 cm | * **Minimum FOV**: 5 x 5 cm | ||
| * **Lens**: f/.95 – f/16, 50 mm | * **Lens**: f/.95 – f/16, 50 mm | ||
| * **Sensitivity**: 100 photons/s/cm²/sr | * **Sensitivity**: 100 photons/s/cm²/sr | ||
| - | * **Read noise**: < 3 electrons for bin = 1, 2, 4; < 5 electrons for bin = 8, 16 | + | * **Read noise**: < 3 electrons for bin = 1, 2, 4; < 5 electrons for bin = 8, 16 <= CONFIRM WITH STAFF! |
| * **Dark Current**: < 120 electrons/s/cm2; or 2 x 10-4 electrons/s/pixel (typical) | * **Dark Current**: < 120 electrons/s/cm2; or 2 x 10-4 electrons/s/pixel (typical) | ||
| * **Excitation fluorescence filters**: 10 | * **Excitation fluorescence filters**: 10 | ||
| * **Emmission fluorescence filters**: 4 | * **Emmission fluorescence filters**: 4 | ||
| + | * **Linear regime of camera is between 600 - 60 000 counts** (avoid operating above 60k!) | ||
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| ==== Excitation and Emission Filtersets ==== | ==== Excitation and Emission Filtersets ==== | ||