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dragonfly [2026/01/20 16:09] bioimaging |
dragonfly [2026/06/05 16:47] (current) bioimaging |
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| <b>Manufacturer</b>: <a href="https://andor.oxinst.com/" target="_blank">Oxford Institutes - Andor</a> <br> | <b>Manufacturer</b>: <a href="https://andor.oxinst.com/" target="_blank">Oxford Institutes - Andor</a> <br> | ||
| <b>Model</b>: <a href="https://andor.oxinst.com/products/dragonfly-confocal-microscope-system" target="_blank">Andor Dragonfly 200</a> <br> | <b>Model</b>: <a href="https://andor.oxinst.com/products/dragonfly-confocal-microscope-system" target="_blank">Andor Dragonfly 200</a> <br> | ||
| - | <b>Nickname</b>: Dragonfly <br> | + | <b>Nickname</b>: Dragonfly<br> |
| - | <b>Software</b>: Fusion <br> | + | <b>Software</b>: Fusion<br> |
| - | <b>Year</b>: 2021 <br> | + | <b>Year</b>: 2021<br> |
| - | <b>SN</b>: <br> | + | <b>SN</b>: CS-02130<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
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| ===== Turn On Procedure ===== | ===== Turn On Procedure ===== | ||
| - | 1. Turn on the Computer (if needed) | + | * Turn on power bar #1. |
| + | * Turn on power bar #2. | ||
| + | * Turn on the LASER key | ||
| + | * Turn on the incubation power bar (if you are planning on using the incubator and/or CO2). | ||
| + | * Turn on the computer. | ||
| + | * Log in Windows with your Agendo credentials. | ||
| + | * Start the **Fusion** software | ||
| - | 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | + | If the system is already on: |
| - | + | * Log in Windows with your Agendo credentials. | |
| - | 3. Turn on laser key | + | * Start the **Fusion** software |
| - | + | ||
| - | 4. (Optional) Turn on incubator powerbar if you are planning to use the incubator and/or the CO2 | + | |
| - | + | ||
| - | 5. (Optional) Turn on the valves for CO2 and Nitrogen mixture, if you planning to use CO2 on your experiment | + | |
| - | + | ||
| - | 6. Login in Agendo and open the Fusion software. | + | |
| - | + | ||
| - | 7. Please ask assistance in advance, if you are going to use either the micropump system or/and the incubation setup. | + | |
| ===== Turn Off Procedure ===== | ===== Turn Off Procedure ===== | ||
| - | 1.Close the software and save all your files to the “files1” server (shortcut in desktop). DO NOT CONNECT external disks/USB flash drives to the workstation! Check if you are the last user in Agendo and if you’re not, proceed to step 5. | + | If there is another user in the next hour: |
| - | + | * Close Fusion | |
| - | 2. Turn the laser key to the Off position | + | * Log off the computer |
| - | + | * Clean up immersion objectives | |
| - | 3. Turn off both the power bars in the back of the microscope table | + | |
| - | + | ||
| - | 4. Clean any immersion objectives used. Use ONLY lens paper provided by staff, NEVER use other types of paper (eg lab towels) or cotton swabs. Apply a few drops of cleaning solution (EtOH) to the lens paper and gently swipe the tip of the objective in a linear motion without pressure (no circular motion). Repeat with a different part of the paper to remove all oil residue. | + | |
| - | 5. Take your belongings (slides, pipettes, etc...); there's a glass disposal bin in the lab! | + | Else: |
| + | * Close Fusion | ||
| + | * Turn off the computer | ||
| + | * Turn off the incubation controller (if used) | ||
| + | * Switch the LASER key off | ||
| + | * Turn off power bars #1 and #2 | ||
| + | * Clean up immersion objectives | ||
| - | 6. Log off your computer session. | ||
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| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||