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dragonfly [2026/01/18 19:27] bioimaging |
dragonfly [2026/06/05 16:47] (current) bioimaging |
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| <b>Manufacturer</b>: <a href="https://andor.oxinst.com/" target="_blank">Oxford Institutes - Andor</a> <br> | <b>Manufacturer</b>: <a href="https://andor.oxinst.com/" target="_blank">Oxford Institutes - Andor</a> <br> | ||
| <b>Model</b>: <a href="https://andor.oxinst.com/products/dragonfly-confocal-microscope-system" target="_blank">Andor Dragonfly 200</a> <br> | <b>Model</b>: <a href="https://andor.oxinst.com/products/dragonfly-confocal-microscope-system" target="_blank">Andor Dragonfly 200</a> <br> | ||
| - | <b>Nickname</b>: Dragonfly <br> | + | <b>Nickname</b>: Dragonfly<br> |
| - | <b>Software</b>: Fusion <br> | + | <b>Software</b>: Fusion<br> |
| - | <b>Year</b>: 2021 <br> | + | <b>Year</b>: 2021<br> |
| - | <b>SN</b>: <br> | + | <b>SN</b>: CS-02130<br> |
|   <br> |   <br> | ||
| Data will be deleted after: <b>1 month</b> | Data will be deleted after: <b>1 month</b> | ||
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| * **Microscope**: [[https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti2-series/|Nikon Ti2 Eclipse]] | * **Microscope**: [[https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti2-series/|Nikon Ti2 Eclipse]] | ||
| - | * **Confocal scanner**: [[https://www.yokogawa.com/eu/solutions/products-and-services/life-science/spinning-disk-confocal/csu-w1-confocal-scanner-unit/|Yokogawa CSU-W1]] + [[https://www.yokogawa.com/solutions/products-and-services/life-science/spinning-disk-confocal/uniformizer/#Overview|Uniformizer]] | + | * **Confocal scanner**: [[https://andor.oxinst.com/products/dragonfly-confocal-microscope-system|Andor Dragonfly 200]] |
| * **Camera**: [[https://andor.oxinst.com/assets/uploads/products/andor/documents/andor-sona-scmos-specifications.pdf|Andor Sona-4BV11]] | * **Camera**: [[https://andor.oxinst.com/assets/uploads/products/andor/documents/andor-sona-scmos-specifications.pdf|Andor Sona-4BV11]] | ||
| + | |||
| + | {{:warning.png?25|}} Data files older than **1 month** will be automatically deleted on this system, please copy your data to the GIMM server using the desktop link. | ||
| ===== System components ===== | ===== System components ===== | ||
| + | ==== LASERs ==== | ||
| - | ==== Lasers ==== | + | ^ LASER ^ Wavelength ^ Maximum Power ^ Measured after objective @ 100% ^ |
| + | | Violet | <fc #a600ff>405 nm</fc> | 100 mW | 7 mW | | ||
| + | | Blue | <fc #00f7ff>488 nm</fc> | 100 mW | 17 mW | | ||
| + | | Yellow-Green | <fc #c6ff00>561 nm</fc> | 100 mW | 20 mW | | ||
| + | | Red | <fc #ff2100>640 nm</fc> | 100 mW | 17 mW | | ||
| - | ^ Laser ^ lines ^ Maximum power ^ Measured at objective @100% (mW) ^ | + | ==== Objectives ==== |
| - | | 405 nm | 100 mW | 7 | | + | |
| - | | 488 nm | 100 mW | 17 | | + | |
| - | | 561 nm | 100 mW | 20 | | + | |
| - | | 640 nm | 100 mW | 17 | | + | |
| + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Pixel Size (zoom 1x) ^ PFS Compatible ^ Reference ^ | ||
| + | | <fc #fef042>10x</fc> | CFI Plan Fluor | Air | 0.30 | 16 | 1.3 mm | 25 μm | 1.0561 μm | Yes | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|MRH00105]] | | ||
| + | | <fc #0aa05c>20x</fc> | CFI Plan Apochromat | Air | 0.8 | 0.8 | 0.68 mm | 40 μm | 0.5097 μm | Yes | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|MRD70270]] | | ||
| + | | <fc #01b3f5>40x</fc> | CFI Plan Apochromat λD | Air | 0.95 | 0.21 | 0.34 mm | 40 μm | 0.2580 μm | Yes | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179806|MRD70470]] | | ||
| + | | <fc #2c80c0>60x</fc> | CFI Plan Apochromat VC | Water | 1.2 | 0.28-0.31 | 0.23 mm | 40 μm | 0.1719 μm | Yes | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1855|MRD07602]] | | ||
| - | ==== Emission Filterwheel Spinning Disk ==== | + | **Upon request:** |
| - | + | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Pixel Size (zoom 1x) ^ PFS Compatible ^ Reference ^ | |
| + | | <fc #0aa05c>20x</fc> | CFI Plan Fluor | 0.75 | multi | 0.33-0.51 | 0.68 mm | 25 μm | 0.5097 μm | No | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1827|MRH07241]] | | ||
| + | | <fc #01b3f5>40x</fc> | CFI Apochromat LWD λS | 1.15 | Water | 0.59-0.61 | 0.34 mm | 40 μm | 0.2580 μm | Yes | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-5994|MRD77410]] | | ||
| + | | 100x | CFI Plan Apochromat λD | 1.45 | Oil | 0.13 | 0.13 mm | 40 μm | 0.1038 μm | Yes | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179810|MRD71970]] | | ||
| - | | Label ^ Transmission (nm) ^ Description (use for): ^ CSU dichroic ^ | + | ==== Emission Filters Spinning Disk ==== |
| - | | Quad (pos0) | 445/521/594/698 | (faster switching) | 405/488/561/640 | | + | |
| - | | 405 (pos1) | 445/46 nm | DAPI, Hoechst | 405/488/561/640 | | + | |
| - | | 488 (pos2) | 521/38 nm | GFP, FITC, Alexa488 | 405/488/561/640 | | + | |
| - | | 561 (pos3) | 594/43 nm | A568 | 405/488/561/640 | | + | |
| - | | 637 (pos4) | 698/77 nm | Cy5, A633, A647 | 405/488/561/640 | | + | |
| - | | 561 extra (pos8) | 606/64 nm | mCherry | 405/488/561/640 | | + | |
| - | | Pol (pos5) | All (50%) | TR-POLR-DIC-FWL | N/A- | | + | |
| - | + | ^ Position ^ Filter name ^ Dichroic ^ Transmission ^ | |
| - | ==== Objectives ==== | + | | 0 | QUAD | 405/488/561/640 | <fc #0000ff>445 nm</fc> | |
| - | + | | ::: | ::: | ::: | <fc #3aff00>521 nm</fc> | | |
| - | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Measured pixel size in μm (1x zoom) ^ PFS Compatible ^ | + | | ::: | ::: | ::: | <fc #ffd200>594 nm</fc> | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.0561 | Yes | | + | | ::: | ::: | ::: | <fc #ff0000>698 nm</fc> | |
| - | | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.5097 | Yes | | + | | 1 | 405 | 405/488/561/640 | <fc #0028ff>422-468 nm</fc> | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179806|40x]] | 0.95 | Dry | 0.25-0.17 | 0.34mm | 40um | 0.34/0.13 | 0.2580 | Yes | | + | | 2 | 488 | 405/488/561/640 | <fc #3aff00>502-540 nm</fc> | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1855|60x]] | 1.2 | Water or Sil | 0.31-0.28 | 0.23mm | 40um | 0.27/0.11 | 0.1719 | Yes | | + | | 3 | 561 | 405/488/561/640 | <fc #ffd200>573-616 nm</fc> | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1827|20x]]* | 0.75 | Multi | 0.51-0.33 | 0.68mm | 25um | 0.43/0.17 | 0.5097 | No | | + | | 4 | 637 | 405/488/561/640 | <fc #ff0000>660-724 nm</fc> | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-5994|40x]]* | 1.15 | Water/Sil | 0.59-0.61 | 0.34mm | 40um | 0.28/0.11 | 0.2580 | Yes | | + | | 5 | Pol | -- | -- | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179810|100x]]* | 1.45 | Oil | 0.13 | 0.13mm | 40um | 0.22/0.09 | 0.1038 | Yes | | + | | 8 | 561 extra | 405/488/561/640 | <fc #4aff00>574-638 nm</fc> | |
| - | + | ||
| - | *Optional | + | |
| ===== Turn On Procedure ===== | ===== Turn On Procedure ===== | ||
| - | 1. Turn on the Computer (if needed) | + | * Turn on power bar #1. |
| + | * Turn on power bar #2. | ||
| + | * Turn on the LASER key | ||
| + | * Turn on the incubation power bar (if you are planning on using the incubator and/or CO2). | ||
| + | * Turn on the computer. | ||
| + | * Log in Windows with your Agendo credentials. | ||
| + | * Start the **Fusion** software | ||
| - | 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | + | If the system is already on: |
| - | + | * Log in Windows with your Agendo credentials. | |
| - | 3. Turn on laser key | + | * Start the **Fusion** software |
| - | + | ||
| - | 4. (Optional) Turn on incubator powerbar if you are planning to use the incubator and/or the CO2 | + | |
| - | + | ||
| - | 5. (Optional) Turn on the valves for CO2 and Nitrogen mixture, if you planning to use CO2 on your experiment | + | |
| - | + | ||
| - | 6. Login in Agendo and open the Fusion software. | + | |
| - | + | ||
| - | 7. Please ask assistance in advance, if you are going to use either the micropump system or/and the incubation setup. | + | |
| ===== Turn Off Procedure ===== | ===== Turn Off Procedure ===== | ||
| - | 1.Close the software and save all your files to the “files1” server (shortcut in desktop). DO NOT CONNECT external disks/USB flash drives to the workstation! Check if you are the last user in Agendo and if you’re not, proceed to step 5. | + | If there is another user in the next hour: |
| - | + | * Close Fusion | |
| - | 2. Turn the laser key to the Off position | + | * Log off the computer |
| - | + | * Clean up immersion objectives | |
| - | 3. Turn off both the power bars in the back of the microscope table | + | |
| - | + | ||
| - | 4. Clean any immersion objectives used. Use ONLY lens paper provided by staff, NEVER use other types of paper (eg lab towels) or cotton swabs. Apply a few drops of cleaning solution (EtOH) to the lens paper and gently swipe the tip of the objective in a linear motion without pressure (no circular motion). Repeat with a different part of the paper to remove all oil residue. | + | |
| - | 5. Take your belongings (slides, pipettes, etc...); there's a glass disposal bin in the lab! | + | Else: |
| + | * Close Fusion | ||
| + | * Turn off the computer | ||
| + | * Turn off the incubation controller (if used) | ||
| + | * Switch the LASER key off | ||
| + | * Turn off power bars #1 and #2 | ||
| + | * Clean up immersion objectives | ||
| - | 6. Log off your computer session. | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
dragonfly.1768760874.txt.gz · Last modified: 2026/01/18 19:27 by bioimaging
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