dragonfly
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dragonfly [2025/06/23 11:54] bioimaging |
dragonfly [2025/08/01 13:45] (current) bioimaging |
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| - | ^ Laser lines ^ Maximum power ^ Measured at objective @100% (mW) ^ | + | ^ Laser ^ lines ^ Maximum power ^ Measured at objective @100% (mW) ^ |
| | 405 nm | 100 mW | 7 | | | 405 nm | 100 mW | 7 | | ||
| | 488 nm | 100 mW | 17 | | | 488 nm | 100 mW | 17 | | ||
| Line 50: | Line 50: | ||
| - | | Filter “Name” ^ Transmission (nm) ^ Description (use for): ^ CSU dichroic ^ | + | | Label ^ Transmission (nm) ^ Description (use for): ^ CSU dichroic ^ |
| | Quad (pos0) | 445/521/594/698 | (faster switching) | 405/488/561/640 | | | Quad (pos0) | 445/521/594/698 | (faster switching) | 405/488/561/640 | | ||
| | 405 (pos1) | 445/46 nm | DAPI, Hoechst | 405/488/561/640 | | | 405 (pos1) | 445/46 nm | DAPI, Hoechst | 405/488/561/640 | | ||
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| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Measured pixel size in μm (1x zoom) ^ PFS Compatible ^ | + | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Measured pixel size in μm (1x zoom) ^ PFS Compatible ^ |
| | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.0561 | Yes | | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.0561 | Yes | | ||
| | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.5097 | Yes | | | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.5097 | Yes | | ||
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| ===== Turn On Procedure ===== | ===== Turn On Procedure ===== | ||
| - | 1. Turn on the Computer | + | 1. Turn on the Computer (if needed) |
| 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | ||
dragonfly.1750672453.txt.gz · Last modified: 2025/06/23 11:54 by bioimaging
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