dragonfly
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dragonfly [2025/05/08 16:36] bioimaging |
dragonfly [2025/08/01 13:45] (current) bioimaging |
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| - | ^ Laser lines ^ Maximum power ^ Measured at objective @100% (mW) ^ | + | ^ Laser ^ lines ^ Maximum power ^ Measured at objective @100% (mW) ^ |
| | 405 nm | 100 mW | 7 | | | 405 nm | 100 mW | 7 | | ||
| | 488 nm | 100 mW | 17 | | | 488 nm | 100 mW | 17 | | ||
| Line 50: | Line 50: | ||
| - | | Filter “Name” ^ Transmission (nm) ^ Description (use for): ^ CSU dichroic ^ | + | | Label ^ Transmission (nm) ^ Description (use for): ^ CSU dichroic ^ |
| | Quad (pos0) | 445/521/594/698 | (faster switching) | 405/488/561/640 | | | Quad (pos0) | 445/521/594/698 | (faster switching) | 405/488/561/640 | | ||
| | 405 (pos1) | 445/46 nm | DAPI, Hoechst | 405/488/561/640 | | | 405 (pos1) | 445/46 nm | DAPI, Hoechst | 405/488/561/640 | | ||
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| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Pixel size in μm (1x / 2x zoom) ^ PFS Compatible ^ | + | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Measured pixel size in μm (1x zoom) ^ PFS Compatible ^ |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.0195 / 0.5097 | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.0561 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.5097 / 0.2549 | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.5097 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179806|40x]] | 0.95 | Dry | 0.25-0.17 | 0.34mm | 40um | 0.34/0.13 | 0.2549 / 0.1274* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179806|40x]] | 0.95 | Dry | 0.25-0.17 | 0.34mm | 40um | 0.34/0.13 | 0.2580 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1855|60x]] | 1.2 | Water or Sil | 0.31-0.28 | 0.23mm | 40um | 0.27/0.11 | 0.1699 / 0.085* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1855|60x]] | 1.2 | Water or Sil | 0.31-0.28 | 0.23mm | 40um | 0.27/0.11 | 0.1719 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1827|20x]]* | 0.75 | Multi | 0.51-0.33 | 0.68mm | 25um | 0.43/0.17 | 0.5097 / 0.2549 | No | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1827|20x]]* | 0.75 | Multi | 0.51-0.33 | 0.68mm | 25um | 0.43/0.17 | 0.5097 | No | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-5994|40x]]* | 1.15 | Water/Sil | 0.59-0.61 | 0.34mm | 40um | 0.28/0.11 | 0.2549 / 0.1274* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-5994|40x]]* | 1.15 | Water/Sil | 0.59-0.61 | 0.34mm | 40um | 0.28/0.11 | 0.2580 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179810|100x]]* | 1.45 | Oil | 0.13 | 0.13mm | 40um | 0.22/0.09 | 0.1019 / 0.051* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179810|100x]]* | 1.45 | Oil | 0.13 | 0.13mm | 40um | 0.22/0.09 | 0.1038 | Yes | |
| *Optional | *Optional | ||
| Line 75: | Line 75: | ||
| ===== Turn On Procedure ===== | ===== Turn On Procedure ===== | ||
| - | 1. Turn on the Computer | + | 1. Turn on the Computer (if needed) |
| 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | ||
dragonfly.1746714963.txt.gz · Last modified: 2025/05/08 16:36 by bioimaging
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