dragonfly
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dragonfly [2025/01/14 16:07] bioimaging |
dragonfly [2025/07/19 21:39] (current) bioimaging |
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| This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 40um). | This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 40um). | ||
| The scanhead allows 1x or 2x magnification (for extra magnification and to allow Nyquist sampling and deconvolution - see table below). | The scanhead allows 1x or 2x magnification (for extra magnification and to allow Nyquist sampling and deconvolution - see table below). | ||
| + | |||
| + | Camera: [[https://andor.oxinst.com/assets/uploads/products/andor/documents/andor-sona-scmos-specifications.pdf|Andor Sona-4BV11]] | ||
| ===== System components ===== | ===== System components ===== | ||
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| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Pixel size in μm (1x / 2x zoom) ^ PFS Compatible ^ | + | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Measured pixel size in μm (1x zoom) ^ PFS Compatible ^ |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.020 / 0.510 | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.0561 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.510 / 0.255 | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.5097 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179806|40x]] | 0.95 | Dry | 0.25-0.17 | 0.34mm | 40um | 0.34/0.13 | 0.255 / 0.108* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179806|40x]] | 0.95 | Dry | 0.25-0.17 | 0.34mm | 40um | 0.34/0.13 | 0.2580 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1855|60x]] | 1.2 | Water or Sil | 0.31-0.28 | 0.23mm | 40um | 0.27/0.11 | 0.170 / 0.085* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1855|60x]] | 1.2 | Water or Sil | 0.31-0.28 | 0.23mm | 40um | 0.27/0.11 | 0.1719 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1827|20x]]* | 0.75 | Multi | 0.51-0.33 | 0.68mm | 25um | 0.43/0.17 | 0.510 / 0.255 | No | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1827|20x]]* | 0.75 | Multi | 0.51-0.33 | 0.68mm | 25um | 0.43/0.17 | 0.5097 | No | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-5994|40x]]* | 1.15 | Water/Sil | 0.59-0.61 | 0.34mm | 40um | 0.28/0.11 | 0.255 / 0.108* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-5994|40x]]* | 1.15 | Water/Sil | 0.59-0.61 | 0.34mm | 40um | 0.28/0.11 | 0.2580 | Yes | |
| - | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179810|100x]]* | 1.45 | Oil | 0.13 | 0.13mm | 40um | 0.22/0.09 | 0.102 / 0.050* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179810|100x]]* | 1.45 | Oil | 0.13 | 0.13mm | 40um | 0.22/0.09 | 0.1038 | Yes | |
| *Optional | *Optional | ||
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| ===== Turn On Procedure ===== | ===== Turn On Procedure ===== | ||
| - | 1. Turn on the Computer | + | 1. Turn on the Computer (if needed) |
| 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | ||
dragonfly.1736867260.txt.gz · Last modified: 2025/01/14 16:07 by bioimaging
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