dragonfly
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dragonfly [2025/01/14 15:35] bioimaging |
dragonfly [2025/07/19 21:39] (current) bioimaging |
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| This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 40um). | This system was built to be versatile, and allow imaging of different sample sizes. It includes both high and low magnification objectives (which require different pinhole sizes, 25 or 40um). | ||
| The scanhead allows 1x or 2x magnification (for extra magnification and to allow Nyquist sampling and deconvolution - see table below). | The scanhead allows 1x or 2x magnification (for extra magnification and to allow Nyquist sampling and deconvolution - see table below). | ||
| + | |||
| + | Camera: [[https://andor.oxinst.com/assets/uploads/products/andor/documents/andor-sona-scmos-specifications.pdf|Andor Sona-4BV11]] | ||
| ===== System components ===== | ===== System components ===== | ||
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| - | | Filter “Name” ^ Transmission (nm) ^ Description (use for): ^ CSU dichroic ^ Link ^ | + | | Filter “Name” ^ Transmission (nm) ^ Description (use for): ^ CSU dichroic ^ |
| - | | Quad (pos0) | 445/521/594/698 | (faster switching) | 405/488/561/640 | link | | + | | Quad (pos0) | 445/521/594/698 | (faster switching) | 405/488/561/640 | |
| - | | 405 (pos1) | 445/46 nm | DAPI, Hoechst | 405/488/561/640 | link | | + | | 405 (pos1) | 445/46 nm | DAPI, Hoechst | 405/488/561/640 | |
| - | | 488 (pos2) | 521/38 nm | GFP, FITC, Alexa488 | 405/488/561/640 | link | | + | | 488 (pos2) | 521/38 nm | GFP, FITC, Alexa488 | 405/488/561/640 | |
| - | | 561 (pos3) | 594/43 nm | A568 | 405/488/561/640 | link | | + | | 561 (pos3) | 594/43 nm | A568 | 405/488/561/640 | |
| - | | 637 (pos4) | 698/77 nm | Cy5, A633, A647 | 405/488/561/640 | link | | + | | 637 (pos4) | 698/77 nm | Cy5, A633, A647 | 405/488/561/640 | |
| - | | 561 extra (pos8) | 606/64 nm | mCherry | 405/488/561/640 | | | + | | 561 extra (pos8) | 606/64 nm | mCherry | 405/488/561/640 | |
| - | | Pol (pos5) | All (50%) | TR-POLR-DIC-FWL | N/A- | N/A | | + | | Pol (pos5) | All (50%) | TR-POLR-DIC-FWL | N/A- | |
| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um | Pixel size in μm (1x / 2x zoom) ^ PFS Compatible ^ | + | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Measured pixel size in μm (1x zoom) ^ PFS Compatible ^ |
| - | | 10x | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.020 / 0.510 | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.0561 | Yes | |
| - | | 20x | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.510 / 0.255 | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.5097 | Yes | |
| - | | 40x | 0.95 | Dry | 0.25-0.17 | 0.34mm | 40um | 0.34/0.13 | 0.255 / 0.108* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179806|40x]] | 0.95 | Dry | 0.25-0.17 | 0.34mm | 40um | 0.34/0.13 | 0.2580 | Yes | |
| - | | 60x | 1.2 | Water or Sil | 0.31-0.28 | 0.23mm | 40um | 0.27/0.11 | 0.170 / 0.085* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1855|60x]] | 1.2 | Water or Sil | 0.31-0.28 | 0.23mm | 40um | 0.27/0.11 | 0.1719 | Yes | |
| - | | 20x | 0.75 | Multi | 0.51-0.33 | 0.68mm | 25um | 0.43/0.17 | 0.510 / 0.255 | No | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1827|20x]]* | 0.75 | Multi | 0.51-0.33 | 0.68mm | 25um | 0.43/0.17 | 0.5097 | No | |
| - | | 40x | 1.15 | Water/Sil | 0.59-0.61 | 0.34mm | 40um | 0.28/0.11 | 0.255 / 0.108* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-5994|40x]]* | 1.15 | Water/Sil | 0.59-0.61 | 0.34mm | 40um | 0.28/0.11 | 0.2580 | Yes | |
| - | | 100x | 1.45 | Oil | 0.13 | 0.13mm | 40um | 0.22/0.09 | 0.102 / 0.050* | Yes | | + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179810|100x]]* | 1.45 | Oil | 0.13 | 0.13mm | 40um | 0.22/0.09 | 0.1038 | Yes | |
| + | |||
| + | *Optional | ||
| ===== Turn On Procedure ===== | ===== Turn On Procedure ===== | ||
| - | 1. Turn on the Computer | + | 1. Turn on the Computer (if needed) |
| 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | ||
dragonfly.1736865309.txt.gz · Last modified: 2025/01/14 15:35 by bioimaging
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