dragonfly
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dragonfly [2025/01/14 11:52] bioimaging |
dragonfly [2025/01/14 16:07] (current) bioimaging |
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| ===== System components ===== | ===== System components ===== | ||
| - | ^ Position ^ Filterset ^ Reference ^ Excitation ^ Dichroic ^ Emission ^ | + | ==== Lasers ==== |
| - | | | | | | | | | + | |
| + | |||
| + | ^ Laser lines ^ Maximum power ^ Measured at objective @100% (mW) ^ | ||
| + | | 405 nm | 100 mW | 7 | | ||
| + | | 488 nm | 100 mW | 17 | | ||
| + | | 561 nm | 100 mW | 20 | | ||
| + | | 640 nm | 100 mW | 17 | | ||
| + | |||
| + | |||
| + | ==== Emission Filterwheel Spinning Disk ==== | ||
| + | |||
| + | |||
| + | | Filter “Name” ^ Transmission (nm) ^ Description (use for): ^ CSU dichroic ^ | ||
| + | | Quad (pos0) | 445/521/594/698 | (faster switching) | 405/488/561/640 | | ||
| + | | 405 (pos1) | 445/46 nm | DAPI, Hoechst | 405/488/561/640 | | ||
| + | | 488 (pos2) | 521/38 nm | GFP, FITC, Alexa488 | 405/488/561/640 | | ||
| + | | 561 (pos3) | 594/43 nm | A568 | 405/488/561/640 | | ||
| + | | 637 (pos4) | 698/77 nm | Cy5, A633, A647 | 405/488/561/640 | | ||
| + | | 561 extra (pos8) | 606/64 nm | mCherry | 405/488/561/640 | | ||
| + | | Pol (pos5) | All (50%) | TR-POLR-DIC-FWL | N/A- | | ||
| ==== Objectives ==== | ==== Objectives ==== | ||
| - | ^ Magnification ^ Model ^ Immersion ^ NA ^ WD (mm) ^ Reference ^ | ||
| - | | | | | | | | ||
| - | ==== Camera ==== | + | ^ Objectives ^ NA ^ Imm. ^ WD (mm) ^ Max FoV (1600x1350px) ^ ideal CSU pinhole ^ Theor. Resol. um ^ Pixel size in μm (1x / 2x zoom) ^ PFS Compatible ^ |
| + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1825|10x]] | 0.3 | Dry | 16 | 1.3mm | 25um | 1.07/0.43 | 1.020 / 0.510 | Yes | | ||
| + | | [[https://www.microscope.healthcare.nikon.com/products/optics/selector/comparison/-179802|20x]] | 0.8 | Dry | 0.8 | 0.68mm | 40um | 0.40/0.16 | 0.510 / 0.255 | Yes | | ||
| + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179806|40x]] | 0.95 | Dry | 0.25-0.17 | 0.34mm | 40um | 0.34/0.13 | 0.255 / 0.108* | Yes | | ||
| + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1855|60x]] | 1.2 | Water or Sil | 0.31-0.28 | 0.23mm | 40um | 0.27/0.11 | 0.170 / 0.085* | Yes | | ||
| + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-1827|20x]]* | 0.75 | Multi | 0.51-0.33 | 0.68mm | 25um | 0.43/0.17 | 0.510 / 0.255 | No | | ||
| + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-5994|40x]]* | 1.15 | Water/Sil | 0.59-0.61 | 0.34mm | 40um | 0.28/0.11 | 0.255 / 0.108* | Yes | | ||
| + | | [[https://www.microscope.healthcare.nikon.com/pt_AMS/products/optics/selector/comparison/-179810|100x]]* | 1.45 | Oil | 0.13 | 0.13mm | 40um | 0.22/0.09 | 0.102 / 0.050* | Yes | | ||
| + | |||
| + | *Optional | ||
| + | |||
| + | ===== Turn On Procedure ===== | ||
| + | |||
| + | 1. Turn on the Computer | ||
| + | |||
| + | 2. Turn on power bar #1 and then power bar #2. These power bars are behind the microscope table, on a rail just above the floor. | ||
| + | |||
| + | 3. Turn on laser key | ||
| + | |||
| + | 4. (Optional) Turn on incubator powerbar if you are planning to use the incubator and/or the CO2 | ||
| + | |||
| + | 5. (Optional) Turn on the valves for CO2 and Nitrogen mixture, if you planning to use CO2 on your experiment | ||
| + | |||
| + | 6. Login in Agendo and open the Fusion software. | ||
| + | |||
| + | 7. Please ask assistance in advance, if you are going to use either the micropump system or/and the incubation setup. | ||
| + | |||
| + | |||
| + | ===== Turn Off Procedure ===== | ||
| + | |||
| + | 1.Close the software and save all your files to the “files1” server (shortcut in desktop). DO NOT CONNECT external disks/USB flash drives to the workstation! Check if you are the last user in Agendo and if you’re not, proceed to step 5. | ||
| + | |||
| + | 2. Turn the laser key to the Off position | ||
| + | |||
| + | 3. Turn off both the power bars in the back of the microscope table | ||
| + | |||
| + | 4. Clean any immersion objectives used. Use ONLY lens paper provided by staff, NEVER use other types of paper (eg lab towels) or cotton swabs. Apply a few drops of cleaning solution (EtOH) to the lens paper and gently swipe the tip of the objective in a linear motion without pressure (no circular motion). Repeat with a different part of the paper to remove all oil residue. | ||
| + | |||
| + | 5. Take your belongings (slides, pipettes, etc...); there's a glass disposal bin in the lab! | ||
| + | 6. Log off your computer session. | ||
| ---- | ---- | ||
| [[resources|Back to the Equipment page]] | [[resources|Back to the Equipment page]] | ||
dragonfly.1736851940.txt.gz · Last modified: 2025/01/14 11:52 by bioimaging
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